KUMAR, Atul, Petra SÝKOROVÁ, Gabriel DEMO, Pavel DOBEŠ, Pavel HYRŠL a Michaela WIMMEROVÁ. A Novel Fucose-Binding Lectin from Photorhabdus luminescens (PLL) with an Unusual Heptabladed beta-Propeller Tetrameric Structure. The Journal of Biological Chemistry, Bethesda: American Society for Biochemistry and Molecular Biology, 2016, roč. 291, č. 48, s. 25032-25049. ISSN 0021-9258. doi:10.1074/jbc.M115.693473.
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Základní údaje
Originální název A Novel Fucose-Binding Lectin from Photorhabdus luminescens (PLL) with an Unusual Heptabladed beta-Propeller Tetrameric Structure
Autoři KUMAR, Atul (356 Indie, domácí), Petra SÝKOROVÁ (203 Česká republika, domácí), Gabriel DEMO (703 Slovensko, domácí), Pavel DOBEŠ (203 Česká republika, domácí), Pavel HYRŠL (203 Česká republika, domácí) a Michaela WIMMEROVÁ (203 Česká republika, garant, domácí).
Vydání The Journal of Biological Chemistry, Bethesda, American Society for Biochemistry and Molecular Biology, 2016, 0021-9258.
Další údaje
Originální jazyk angličtina
Typ výsledku Článek v odborném periodiku
Obor 1.6 Biological sciences
Stát vydavatele Spojené státy americké
Utajení není předmětem státního či obchodního tajemství
WWW URL
Impakt faktor Impact factor: 4.125
Kód RIV RIV/00216224:14740/16:00088341
Organizační jednotka Středoevropský technologický institut
Doi http://dx.doi.org/10.1074/jbc.M115.693473
UT WoS 000388880100020
Klíčová slova anglicky lectin; bacterial pathogen; host-pathogen interaction; crystal structure; structural biology; Photorhabdus luminescen; haemocytes; Galleria mellonella; nematodes; Heterorhabditis bacteriophora
Štítky rivok
Příznaky Mezinárodní význam, Recenzováno
Změnil Změnila: Mgr. Eva Špillingová, učo 110713. Změněno: 14. 3. 2017 13:18.
Anotace
Photorhabdus luminescens is known for its symbiosis with the entomopathogenic nematode Heterorhabditis bacteriophora and its pathogenicity toward insect larvae. Ahypothetical protein from P. luminescens was identified, purified from the native source, and characterized as an L-fucose-binding lectin, named P. luminescens lectin (PLL). Glycan array and biochemical characterization data revealed PLL to be specific toward L-fucose and the disaccharide glycan 3,6-O-Me-2-Glc beta 1-4(2,3-O-Me-2)Rha alpha-O-(p-C6H4)-OCH2CH2NH2. PLL was discovered to be a homotetramer with an intersubunit disulfide bridge. The crystal structures of native and recombinant PLL revealed a seven-bladed beta-propeller fold creating seven putative fucose-binding sites per monomer. The crystal structure of the recombinant PLL.L-fucose complex confirmed that at least three sites were fucose-binding. Moreover, the crystal structures indicated that some of the other sites are masked either by the tetrameric nature of the lectin or by incorporation of the C terminus of the lectin into one of these sites. PLL exhibited an ability to bind to insect hemocytes and the cuticular surface of a nematode, H. bacteriophora.
Anotace česky
Photorhabdus luminescens is known for its symbiosis with the entomopathogenic nematode Heterorhabditis bacteriophora and its pathogenicity toward insect larvae. Ahypothetical protein from P. luminescens was identified, purified from the native source, and characterized as an L-fucose-binding lectin, named P. luminescens lectin (PLL). Glycan array and biochemical characterization data revealed PLL to be specific toward L-fucose and the disaccharide glycan 3,6-O-Me-2-Glc beta 1-4(2,3-O-Me-2)Rha alpha-O-(p-C6H4)-OCH2CH2NH2. PLL was discovered to be a homotetramer with an intersubunit disulfide bridge. The crystal structures of native and recombinant PLL revealed a seven-bladed beta-propeller fold creating seven putative fucose-binding sites per monomer. The crystal structure of the recombinant PLL.L-fucose complex confirmed that at least three sites were fucose-binding. Moreover, the crystal structures indicated that some of the other sites are masked either by the tetrameric nature of the lectin or by incorporation of the C terminus of the lectin into one of these sites. PLL exhibited an ability to bind to insect hemocytes and the cuticular surface of a nematode, H. bacteriophora.
Návaznosti
EE2.3.30.0009, projekt VaVNázev: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci
GA13-25401S, projekt VaVNázev: Studium proteinů z patogenů zapojených do rozpoznávání hostitelského organismu
Investor: Grantová agentura ČR, Standardní projekty
LQ1601, projekt VaVNázev: CEITEC 2020 (Akronym: CEITEC2020)
Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Národní program udržitelnosti II (LQ)
VytisknoutZobrazeno: 18. 8. 2019 21:24