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@article{1360870, author = {Kumar, Atul and Sýkorová, Petra and Demo, Gabriel and Dobeš, Pavel and Hyršl, Pavel and Wimmerová, Michaela}, article_location = {Bethesda}, article_number = {48}, doi = {http://dx.doi.org/10.1074/jbc.M115.693473}, keywords = {lectin; bacterial pathogen; host-pathogen interaction; crystal structure; structural biology; Photorhabdus luminescen; haemocytes; Galleria mellonella; nematodes; Heterorhabditis bacteriophora}, language = {eng}, issn = {0021-9258}, journal = {The Journal of Biological Chemistry}, title = {A Novel Fucose-Binding Lectin from Photorhabdus luminescens (PLL) with an Unusual Heptabladed beta-Propeller Tetrameric Structure}, url = {http://www.jbc.org/content/291/48/25032}, volume = {291}, year = {2016} }
TY - JOUR ID - 1360870 AU - Kumar, Atul - Sýkorová, Petra - Demo, Gabriel - Dobeš, Pavel - Hyršl, Pavel - Wimmerová, Michaela PY - 2016 TI - A Novel Fucose-Binding Lectin from Photorhabdus luminescens (PLL) with an Unusual Heptabladed beta-Propeller Tetrameric Structure JF - The Journal of Biological Chemistry VL - 291 IS - 48 SP - 25032-25049 EP - 25032-25049 PB - American Society for Biochemistry and Molecular Biology SN - 00219258 KW - lectin KW - bacterial pathogen KW - host-pathogen interaction KW - crystal structure KW - structural biology KW - Photorhabdus luminescen KW - haemocytes KW - Galleria mellonella KW - nematodes KW - Heterorhabditis bacteriophora UR - http://www.jbc.org/content/291/48/25032 L2 - http://www.jbc.org/content/291/48/25032 N2 - Photorhabdus luminescens is known for its symbiosis with the entomopathogenic nematode Heterorhabditis bacteriophora and its pathogenicity toward insect larvae. Ahypothetical protein from P. luminescens was identified, purified from the native source, and characterized as an L-fucose-binding lectin, named P. luminescens lectin (PLL). Glycan array and biochemical characterization data revealed PLL to be specific toward L-fucose and the disaccharide glycan 3,6-O-Me-2-Glc beta 1-4(2,3-O-Me-2)Rha alpha-O-(p-C6H4)-OCH2CH2NH2. PLL was discovered to be a homotetramer with an intersubunit disulfide bridge. The crystal structures of native and recombinant PLL revealed a seven-bladed beta-propeller fold creating seven putative fucose-binding sites per monomer. The crystal structure of the recombinant PLL.L-fucose complex confirmed that at least three sites were fucose-binding. Moreover, the crystal structures indicated that some of the other sites are masked either by the tetrameric nature of the lectin or by incorporation of the C terminus of the lectin into one of these sites. PLL exhibited an ability to bind to insect hemocytes and the cuticular surface of a nematode, H. bacteriophora. ER -
KUMAR, Atul, Petra SÝKOROVÁ, Gabriel DEMO, Pavel DOBEŠ, Pavel HYRŠL a Michaela WIMMEROVÁ. A Novel Fucose-Binding Lectin from Photorhabdus luminescens (PLL) with an Unusual Heptabladed beta-Propeller Tetrameric Structure. \textit{The Journal of Biological Chemistry}. Bethesda: American Society for Biochemistry and Molecular Biology, 2016, roč.~291, č.~48, s.~25032-25049. ISSN~0021-9258. Dostupné z: https://dx.doi.org/10.1074/jbc.M115.693473.
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