| ZAPLETALOVÁ, Martina, Jitka KAŠPAROVSKÁ, Ludmila KŘÍŽOVÁ, Tomáš KAŠPAROVSKÝ, Omar ŠERÝ a Jan LOCHMAN. Bacterial community dynamics in a rumen fluid bioreactor during in-vitro cultivation. Journal of Biotechnology. Amsterdam: Elsevier, 2016, roč. 234, september, s. 43-49. ISSN 0168-1656. Dostupné z: https://dx.doi.org/10.1016/j.jbiotec.2016.07.013. |
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@article{1362002,
author = {Zapletalová, Martina and Kašparovská, Jitka and Křížová, Ludmila and Kašparovský, Tomáš and Šerý, Omar and Lochman, Jan},
article_location = {Amsterdam},
article_number = {september},
doi = {http://dx.doi.org/10.1016/j.jbiotec.2016.07.013},
keywords = {Rumen fluid; Bioreactor; Redox potential; Microbiome analysis; 16S rDNA},
language = {eng},
issn = {0168-1656},
journal = {Journal of Biotechnology},
title = {Bacterial community dynamics in a rumen fluid bioreactor during in-vitro cultivation},
volume = {234},
year = {2016}
}
TY - JOUR
ID - 1362002
AU - Zapletalová, Martina - Kašparovská, Jitka - Křížová, Ludmila - Kašparovský, Tomáš - Šerý, Omar - Lochman, Jan
PY - 2016
TI - Bacterial community dynamics in a rumen fluid bioreactor during in-vitro cultivation
JF - Journal of Biotechnology
VL - 234
IS - september
SP - 43-49
EP - 43-49
PB - Elsevier
SN - 01681656
KW - Rumen fluid
KW - Bioreactor
KW - Redox potential
KW - Microbiome analysis
KW - 16S rDNA
N2 - To study the various processes in the rumen the in vitro techniques are widely used to realize more controlled and reproducible conditions compared to in vivo experiments. Mostly, only the parameters like pH changes, volatile fatty acids content or metabolite production are monitored. In this study we examine the bacterial community dynamics of rumen fluid in course of ten day cultivation realize under standard conditions described in the literature. Whereas the pH values, total VFA content and A/P ratio in bioreactor were consistent with natural conditions in the rumen, the mean redox-potential values of 251 and 243 mV were much more negative. For culture-independent assessment of bacterial community composition, the Illumina MiSeq results indicated that the community contained 292 bacterial genera. In course of ten days cultivation a significant changes in the microbial community were measured when Bacteroidetes to Firmicutes ratio changed from 3.2 to 1.2 and phyla Proteo bacteria and Actino bacteria represented by genus Bifido bacterium and Olsenella significantly increased. The main responsible factor of these changes seems to be very low redox potential in bioreactor together with accumulation of simple carbohydrates in milieu as a result of limited excretion of fermented feed and absence of nutrient absorbing mechanisms. (C) 2016 Elsevier B.V. All rights reserved.
ER -
ZAPLETALOVÁ, Martina, Jitka KAŠPAROVSKÁ, Ludmila KŘÍŽOVÁ, Tomáš KAŠPAROVSKÝ, Omar ŠERÝ a Jan LOCHMAN. Bacterial community dynamics in a rumen fluid bioreactor during in-vitro cultivation. \textit{Journal of Biotechnology}. Amsterdam: Elsevier, 2016, roč.~234, september, s.~43-49. ISSN~0168-1656. Dostupné z: https://dx.doi.org/10.1016/j.jbiotec.2016.07.013.
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