ERLIC or just HILIC? Investigation of chromatographic behavior
of trinucleotides

D 2016

ERLIC or just HILIC? Investigation of chromatographic behavior of trinucleotides

FIALA, Jan; Miroslava BITTOVÁ a Jan HAVLIŠ

Základní údaje

Originální název

ERLIC or just HILIC? Investigation of chromatographic behavior of trinucleotides

Autoři

FIALA, Jan; Miroslava BITTOVÁ a Jan HAVLIŠ

Vydání

Olomouc, Advances in chromatography and electrophoresis & Chiranal 2016, od s. 94-95, 2 s. 2016

Nakladatel

Palacký University, Olomouc

Další údaje

Jazyk

angličtina

Typ výsledku

Stať ve sborníku

Obor

10406 Analytical chemistry

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

tištěná verze "print"

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/16:00092198

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-244-4961-6

Klíčová slova anglicky

HILIC; ERLIC; HPLC; trinucleotides; retention behavior

Změněno: 7. 12. 2016 14:06, Mgr. Miroslava Bittová, Ph.D.

Anotace

V originále

Electrostatic repulsion hydrophilic interaction chromatography (ERLIC) is mixed-mode chromatographic technique which combines to use of electrostatic repulsion and hydrophilic interaction. This chromatographic technique was firstly introduced by J. Alpert in 2008 as an alternative method for separation of highly charged and hydrophilic analytes e.g. peptides, amino acids and nucleotides. Main idea behind ERLIC is based on selective decrease in retention due to repulsion between equally charged stationary phase and analyte. This repulsion effect can be mainly controlled by pH, adjusting presence or absence of charged molecules which result in the isocratic elution of highly retained analytes that would require a gradient in other chromatographic modes such as HILIC or RPLC.

Návaznosti

MUNI/A/1452/2014, interní kód MU

Název: Vývoj metod a instrumentace pro analýzu biologicky významných látek 2015

Investor: Masarykova univerzita, Vývoj metod a instrumentace pro analýzu biologicky významných látek 2015, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

FIALA, Jan; Miroslava BITTOVÁ a Jan HAVLIŠ. ERLIC or just HILIC? Investigation of chromatographic behavior of trinucleotides. In Vítězslav Maier. Advances in chromatography and electrophoresis & Chiranal 2016. Olomouc: Palacký University, Olomouc, 2016, s. 94-95. ISBN 978-80-244-4961-6.

@inproceedings{1363932,
   author = {Fiala, Jan and Bittová, Miroslava and Havliš, Jan},
   address = {Olomouc},
   booktitle = {Advances in chromatography and electrophoresis & Chiranal 2016},
   editor = {Vítězslav Maier},
   keywords = {HILIC; ERLIC; HPLC; trinucleotides; retention behavior},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {Olomouc},
   isbn = {978-80-244-4961-6},
   pages = {94-95},
   publisher = {Palacký University, Olomouc},
   title = {ERLIC or just HILIC? Investigation of chromatographic behavior of trinucleotides},
   year = {2016}
}

TY  - CONF
ID  - 1363932
AU  - Fiala, Jan - Bittová, Miroslava - Havliš, Jan
PY  - 2016
TI  - ERLIC or just HILIC? Investigation of chromatographic behavior of trinucleotides
PB  - Palacký University, Olomouc
CY  - Olomouc
SN  - 9788024449616
KW  - HILIC
KW  - ERLIC
KW  - HPLC
KW  - trinucleotides
KW  - retention behavior
N2  - Electrostatic repulsion hydrophilic interaction chromatography (ERLIC) is mixed-mode chromatographic technique which combines to use of electrostatic repulsion and hydrophilic interaction. This chromatographic technique was firstly introduced by J. Alpert in 2008 as an alternative method for separation of highly charged and hydrophilic analytes e.g. peptides, amino acids and nucleotides. Main idea behind ERLIC is based on selective decrease in retention due to repulsion between equally charged stationary phase and analyte. This repulsion effect can be mainly controlled by pH, adjusting presence or absence of charged molecules which result in the isocratic elution of highly retained analytes that would require a gradient in other chromatographic modes such as HILIC or RPLC.
ER  -

FIALA, Jan; Miroslava BITTOVÁ a Jan HAVLIŠ. ERLIC or just HILIC? Investigation of chromatographic behavior of trinucleotides. In Vítězslav Maier. \textit{Advances in chromatography and electrophoresis \&{} Chiranal 2016}. Olomouc: Palacký University, Olomouc, 2016, s.~94-95. ISBN~978-80-244-4961-6.

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