| MILOSAVLJEVIC, Vedran, Yazan HADDAD, Miguel Angel Merlos RODRIGO, Amitava MOULICK, Hana POLANSKÁ, David HYNEK, Zbynek HEGER, Pavel KOPEL a Vojtech ADAM. The Zinc-Schiff Base-Novicidin Complex as a Potential Prostate Cancer Therapy. Plos one. San Francisco: Public Library of Science, 2016, roč. 11, č. 10, s. 1-19. ISSN 1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0163983. |
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@article{1364186,
author = {Milosavljevic, Vedran and Haddad, Yazan and Rodrigo, Miguel Angel Merlos and Moulick, Amitava and Polanská, Hana and Hynek, David and Heger, Zbynek and Kopel, Pavel and Adam, Vojtech},
article_location = {San Francisco},
article_number = {10},
doi = {http://dx.doi.org/10.1371/journal.pone.0163983},
keywords = {TRANSCRIPTION FACTOR SP1; STEADY-STATE; CELL-LINES; EXPRESSION; APOPTOSIS; ACTIVATION; MICROARRAY; PROTEIN; MODEL; P53},
language = {eng},
issn = {1932-6203},
journal = {Plos one},
title = {The Zinc-Schiff Base-Novicidin Complex as a Potential Prostate Cancer Therapy},
volume = {11},
year = {2016}
}
TY - JOUR
ID - 1364186
AU - Milosavljevic, Vedran - Haddad, Yazan - Rodrigo, Miguel Angel Merlos - Moulick, Amitava - Polanská, Hana - Hynek, David - Heger, Zbynek - Kopel, Pavel - Adam, Vojtech
PY - 2016
TI - The Zinc-Schiff Base-Novicidin Complex as a Potential Prostate Cancer Therapy
JF - Plos one
VL - 11
IS - 10
SP - 1-19
EP - 1-19
PB - Public Library of Science
SN - 19326203
KW - TRANSCRIPTION FACTOR SP1
KW - STEADY-STATE
KW - CELL-LINES
KW - EXPRESSION
KW - APOPTOSIS
KW - ACTIVATION
KW - MICROARRAY
KW - PROTEIN
KW - MODEL
KW - P53
N2 - Prostate cancer cells control energy metabolism by chelating intracellular zinc. Thus, zinc delivery has been a popular therapeutic approach for prostate cancer. Here, we propose the use of the membrane-penetrating peptide Novicidin connected to zinc-Schiff base as a carrier vehicle for the delivery of zinc to prostate cells. Mass spectrometry, electrochemistry and spectrophotometry confirmed the formation/stability of this complex and provided insight regarding the availability of zinc for complex interactions. This delivery system showed minor toxicity in normal PNT1A cells and high potency towards PC3 tumor cells. The complex preferentially penetrated PC3 tumor cells in contrast to confinement to the membranes of PNT1A. Furthermore, zinc uptake was confirmed in both cell lines. Molecular analysis was used to confirm the activation of zinc stress (e.g., ZnT-1) and apoptosis (e.g., CASP-1). Our results strongly suggest that the zinc-Schiff base-Novicidin complex has great potential as a novel anticancer drug.
ER -
MILOSAVLJEVIC, Vedran, Yazan HADDAD, Miguel Angel Merlos RODRIGO, Amitava MOULICK, Hana POLANSKÁ, David HYNEK, Zbynek HEGER, Pavel KOPEL a Vojtech ADAM. The Zinc-Schiff Base-Novicidin Complex as a Potential Prostate Cancer Therapy. \textit{Plos one}. San Francisco: Public Library of Science, 2016, roč.~11, č.~10, s.~1-19. ISSN~1932-6203. Dostupné z: https://dx.doi.org/10.1371/journal.pone.0163983.
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