Expression of integrins and GDF9 mRNAs is associated with
ovarian follicle size and donor puberty status in pigs

J 2016

Expression of integrins and GDF9 mRNAs is associated with ovarian follicle size and donor puberty status in pigs

ANTOSIK, Paveł; Michal JEŠETA; Wiesława KRANC; Adrian CHACHUŁA; Artur BRYJA et al.

Základní údaje

Originální název

Expression of integrins and GDF9 mRNAs is associated with ovarian follicle size and donor puberty status in pigs

Autoři

Vydání

Medycyna Weterynaryjna, Lublin, Polskie Towarzystwo Nauk Weterynaryjnych, 2016, 0025-8628

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

40301 Veterinary science

Stát vydavatele

Polsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 0.161

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/16:00092281

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

GDF9; IGFB; oocyte; pig

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 5. 1. 2017 16:46, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

Ovarian folliculogenesis and oogenesis has a significant impact on embryo growth and development in preimplantation stages. Although both processes are widely understood in several species of mammals, including pigs, the factors influencing the proper maturation capability of oocytes, as well as the developmental competence of the surrounding somatic granulosa cells (GCs) and cumulus cells (CCs), are still not entirely known. This study aimed to investigate the expression of growth differentiation factor 9 (GDF9) and integrins (ITGB1,ITGB2, ITGB3 and ITGB4) in porcine oocytes isolated from follicles of various size and donors characterized by different puberty status. The relative abundance of GDF9, ITGB1, ITGB2, ITGB3, and ITGB4 mRNAs in porcine oocytes isolated from medium follicles of cycling sows (MFCS), small follicles of juvenile gilts (SFJG), and small follicles of cycling sows (SFCS) was assessed by an RT-qPCR assay. It was found an increased expression of GDF9 in oocytes isolated from the small follicles of juvenile gilts as compared to the other two groups (P < 0.001). A significant down-regulation of ITGB1 and ITGB2 oocyte mRNAs collected from medium follicles of cycling sows was observed (P < 0.05 and P < 0.001, respectively). The ITGB3 mRNA expression was significantly decreased in oocytes isolated from small follicles of juvenile gilts (P < 0.001), whereas a lower expression of ITGB4 in oocytes from both medium follicles (cycling sows) and small follicles (juvenile gilts) was observed. In conclusion, GDF9 may be recognized as the main factor regulating follicle growth at early stages of folliculogenesis. The expression of ITGBs is significantly regulated by the puberty status of donor pigs, and different follicular sizes may play a subordinate role in integrin expression during in vivo follicle development in pigs.

Návaznosti

ROZV/24/LF/2016, interní kód MU

Název: LF - Příspěvek IP 2016

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, LF - Příspěvek IP 2016

Citovat

ANTOSIK, Paveł; Michal JEŠETA; Wiesława KRANC; Adrian CHACHUŁA; Artur BRYJA; Joanna BUDNA; Sylwia CIESIÓŁKA; Katarzyna WOJTANOWICZ-MARKIEWICZ; Dorota BUKOWSKA; Klaus-Peter BRÜSSOW; Małgorzata BRUSKA; Michał NOWICKI; Pavlina HULINSKA; Marie MACHATKOVA a Bartocz KEMPISTY. Expression of integrins and GDF9 mRNAs is associated with ovarian follicle size and donor puberty status in pigs. Medycyna Weterynaryjna. Lublin: Polskie Towarzystwo Nauk Weterynaryjnych, 2016, roč. 72, č. 12, s. 750-754. ISSN 0025-8628. Dostupné z: https://doi.org/10.21521/mw.5601.

@article{1364723,
   author = {Antosik, Paveł and Ješeta, Michal and Kranc, Wiesława and Chachuła, Adrian and Bryja, Artur and Budna, Joanna and Ciesiółka, Sylwia and WojtanowiczandMarkiewicz, Katarzyna and Bukowska, Dorota and Brüssow, KlausandPeter and Bruska, Małgorzata and Nowicki, Michał and Hulinska, Pavlina and Machatkova, Marie and Kempisty, Bartocz},
   article_location = {Lublin},
   article_number = {12},
   doi = {https://doi.org/10.21521/mw.5601},
   keywords = {GDF9; IGFB; oocyte; pig},
   language = {eng},
   issn = {0025-8628},
   journal = {Medycyna Weterynaryjna},
   title = {Expression of integrins and GDF9 mRNAs is associated with ovarian follicle size and donor puberty status in pigs},
   volume = {72},
   year = {2016}
}

TY  - JOUR
ID  - 1364723
AU  - Antosik, Paveł - Ješeta, Michal - Kranc, Wiesława - Chachuła, Adrian - Bryja, Artur - Budna, Joanna - Ciesiółka, Sylwia - Wojtanowicz-Markiewicz, Katarzyna - Bukowska, Dorota - Brüssow, Klaus-Peter - Bruska, Małgorzata - Nowicki, Michał - Hulinska, Pavlina - Machatkova, Marie - Kempisty, Bartocz
PY  - 2016
TI  - Expression of integrins and GDF9 mRNAs is associated with ovarian follicle size and donor puberty status in pigs
JF  - Medycyna Weterynaryjna
VL  - 72
IS  - 12
SP  - 750-754
EP  - 750-754
PB  - Polskie Towarzystwo Nauk Weterynaryjnych
SN  - 00258628
KW  - GDF9
KW  - IGFB
KW  - oocyte
KW  - pig
N2  - Ovarian folliculogenesis and oogenesis has a significant impact on embryo growth and development in preimplantation stages. Although both processes are widely understood in several species of mammals, including pigs, the factors influencing the proper maturation capability of oocytes, as well as the developmental competence of the surrounding somatic granulosa cells (GCs) and cumulus cells (CCs), are still not entirely known. This study aimed to investigate the expression of growth differentiation factor 9 (GDF9) and integrins (ITGB1,ITGB2, ITGB3 and ITGB4) in porcine oocytes isolated from follicles of various size and donors characterized by different puberty status. The relative abundance of GDF9, ITGB1, ITGB2, ITGB3, and ITGB4 mRNAs in porcine oocytes isolated from medium follicles of cycling sows (MFCS), small follicles of juvenile gilts (SFJG), and small follicles of cycling sows (SFCS) was assessed by an RT-qPCR assay. It was found an increased expression of GDF9 in oocytes isolated from the small follicles of juvenile gilts as compared to the other two groups (P < 0.001). A significant down-regulation of ITGB1 and ITGB2 oocyte mRNAs collected from medium follicles of cycling sows was observed (P < 0.05 and P < 0.001, respectively). The ITGB3 mRNA expression was significantly decreased in oocytes isolated from small follicles of juvenile gilts (P < 0.001), whereas a lower expression of ITGB4 in oocytes from both medium follicles (cycling sows) and small follicles (juvenile gilts) was observed. In conclusion, GDF9 may be recognized as the main factor regulating follicle growth at early stages of folliculogenesis. The expression of ITGBs is significantly regulated by the puberty status of donor pigs, and different follicular sizes may play a subordinate role in integrin expression during in vivo follicle development in pigs.
ER  -

ANTOSIK, Paveł; Michal JEŠETA; Wiesława KRANC; Adrian CHACHUŁA; Artur BRYJA; Joanna BUDNA; Sylwia CIESIÓŁKA; Katarzyna WOJTANOWICZ-MARKIEWICZ; Dorota BUKOWSKA; Klaus-Peter BRÜSSOW; Małgorzata BRUSKA; Michał NOWICKI; Pavlina HULINSKA; Marie MACHATKOVA a Bartocz KEMPISTY. Expression of integrins and GDF9 mRNAs is associated with ovarian follicle size and donor puberty status in pigs. \textit{Medycyna Weterynaryjna}. Lublin: Polskie Towarzystwo Nauk Weterynaryjnych, 2016, roč.~72, č.~12, s.~750-754. ISSN~0025-8628. Dostupné z: https://doi.org/10.21521/mw.5601.

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