Co-delivery of VP-16 and Bcl-2-targeted antisense on
PEG-grafted oMWCNTs for synergistic in vitro anti-cancer
effects in non-small and small cell lung cancer

J 2017

Co-delivery of VP-16 and Bcl-2-targeted antisense on PEG-grafted oMWCNTs for synergistic in vitro anti-cancer effects in non-small and small cell lung cancer

HEGER, Zbynek; Hana POLANSKÁ; Sona KRIZKOVA; Jan BALVAN; Martina RAUDENSKÁ et. al.

Základní údaje

Originální název

Co-delivery of VP-16 and Bcl-2-targeted antisense on PEG-grafted oMWCNTs for synergistic in vitro anti-cancer effects in non-small and small cell lung cancer

Autoři

HEGER, Zbynek; Hana POLANSKÁ; Sona KRIZKOVA; Jan BALVAN; Martina RAUDENSKÁ ; Simona DOSTALOVA; Amitava MOULICK; Michal MASAŘÍK a Vojtech ADAM

Vydání

Colloids and Surfaces B: Biointerfaces, Amsterdam, Elsevier, 2017, 0927-7765

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30105 Physiology

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.997

Kód RIV

RIV/00216224:14110/17:00095967

Organizační jednotka

Lékařská fakulta

DOI

https://doi.org/10.1016/j.colsurfb.2016.11.023

UT WoS

000393726900016

EID Scopus

2-s2.0-84998881020

Klíčová slova anglicky

Drug delivery; Etoposide; In vitro release; Multi-walled carbon nanotubes; Nanomedicine

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 3. 2018 16:24, Soňa Böhmová

Anotace

V originále

Present study describes the preparation of a polyethylene glycol-grafted oxidized multi-walled carbon nanotubes (oMWCNTs-PEG) hybrid nanosystem as a carrier of etoposide (VP-16) and Bcl-2 phosphorothioate antisense deoxyoligonucleotides (Aso) to achieve a superior cytostastic efficacy in non-small and small cell lung cancer in vitro. We have demonstrated that the adsorption of hydrophobic VP-16 and Bcl-2 Aso results in a stable nanotransporter exhibiting good dispersion with excellent release profiles (both, in pH 7.4 and 4.8) and negligible hemolytic activity (up to 6.5%). The evaluation of cytotoxicity was carried out in in vitro using small cell (SCLC; DMS53) and non-small cell lung cancer (NSCLC; NCIH2135) cell lines. It was found that Bcl-2 interference significantly increased the anti-cancer efficiency of VP-16 in the chemoresistant NSCLC cells. This was further supported using a flow-cytometry (Annexin V/propidium iodide assay), which revealed a significant increase in apoptotic cells in both the cell lines after the co-administration of VP-16 and Bcl-2 Aso using oMWCNTs-PEG hybrid, and fluorescence microscopy, which showed an increase in reactive oxygen species identified after Bcl-2 knock-down. Overall, oMWCNTs-PEG provided an exceptional biocompatible vehicle enabling the internalization of negatively charged nucleic acids and pH-sensitive release of cargoes in a hypoxic environment of the most of solid tumors. Moreover, Aso specifically binding to the first six codons of the Bcl-2 mRNA gave a satisfactorily decrease in Bcl-2 translation and an increase in NCIH2135 chemosensitivity towards VP-16.

Citovat

HEGER, Zbynek; Hana POLANSKÁ; Sona KRIZKOVA; Jan BALVAN; Martina RAUDENSKÁ; Simona DOSTALOVA; Amitava MOULICK; Michal MASAŘÍK a Vojtech ADAM. Co-delivery of VP-16 and Bcl-2-targeted antisense on PEG-grafted oMWCNTs for synergistic in vitro anti-cancer effects in non-small and small cell lung cancer. Colloids and Surfaces B: Biointerfaces. Amsterdam: Elsevier, 2017, roč. 150, 1 February 2017, s. 131-140. ISSN 0927-7765. Dostupné z: https://doi.org/10.1016/j.colsurfb.2016.11.023.

@article{1364900,
   author = {Heger, Zbynek and Polanská, Hana and Krizkova, Sona and Balvan, Jan and Raudenská, Martina and Dostalova, Simona and Moulick, Amitava and Masařík, Michal and Adam, Vojtech},
   article_location = {Amsterdam},
   article_number = {1 February 2017},
   doi = {https://doi.org/10.1016/j.colsurfb.2016.11.023},
   keywords = {Drug delivery; Etoposide; In vitro release; Multi-walled carbon nanotubes; Nanomedicine},
   language = {eng},
   issn = {0927-7765},
   journal = {Colloids and Surfaces B: Biointerfaces},
   title = {Co-delivery of VP-16 and Bcl-2-targeted antisense on PEG-grafted oMWCNTs for synergistic in vitro anti-cancer effects in non-small and small cell lung cancer},
   volume = {150},
   year = {2017}
}

TY  - JOUR
ID  - 1364900
AU  - Heger, Zbynek - Polanská, Hana - Krizkova, Sona - Balvan, Jan - Raudenská, Martina - Dostalova, Simona - Moulick, Amitava - Masařík, Michal - Adam, Vojtech
PY  - 2017
TI  - Co-delivery of VP-16 and Bcl-2-targeted antisense on PEG-grafted oMWCNTs for synergistic in vitro anti-cancer effects in non-small and small cell lung cancer
JF  - Colloids and Surfaces B: Biointerfaces
VL  - 150
IS  - 1 February 2017
SP  - 131-140
EP  - 131-140
PB  - Elsevier
SN  - 09277765
KW  - Drug delivery
KW  - Etoposide
KW  - In vitro release
KW  - Multi-walled carbon nanotubes
KW  - Nanomedicine
N2  - Present study describes the preparation of a polyethylene glycol-grafted oxidized multi-walled carbon nanotubes (oMWCNTs-PEG) hybrid nanosystem as a carrier of etoposide (VP-16) and Bcl-2 phosphorothioate antisense deoxyoligonucleotides (Aso) to achieve a superior cytostastic efficacy in non-small and small cell lung cancer in vitro. We have demonstrated that the adsorption of hydrophobic VP-16 and Bcl-2 Aso results in a stable nanotransporter exhibiting good dispersion with excellent release profiles (both, in pH 7.4 and 4.8) and negligible hemolytic activity (up to 6.5%). The evaluation of cytotoxicity was carried out in in vitro using small cell (SCLC; DMS53) and non-small cell lung cancer (NSCLC; NCIH2135) cell lines. It was found that Bcl-2 interference significantly increased the anti-cancer efficiency of VP-16 in the chemoresistant NSCLC cells. This was further supported using a flow-cytometry (Annexin V/propidium iodide assay), which revealed a significant increase in apoptotic cells in both the cell lines after the co-administration of VP-16 and Bcl-2 Aso using oMWCNTs-PEG hybrid, and fluorescence microscopy, which showed an increase in reactive oxygen species identified after Bcl-2 knock-down. Overall, oMWCNTs-PEG provided an exceptional biocompatible vehicle enabling the internalization of negatively charged nucleic acids and pH-sensitive release of cargoes in a hypoxic environment of the most of solid tumors. Moreover, Aso specifically binding to the first six codons of the Bcl-2 mRNA gave a satisfactorily decrease in Bcl-2 translation and an increase in NCIH2135 chemosensitivity towards VP-16.
ER  -

HEGER, Zbynek; Hana POLANSKÁ; Sona KRIZKOVA; Jan BALVAN; Martina RAUDENSKÁ; Simona DOSTALOVA; Amitava MOULICK; Michal MASAŘÍK a Vojtech ADAM. Co-delivery of VP-16 and Bcl-2-targeted antisense on PEG-grafted oMWCNTs for synergistic in vitro anti-cancer effects in non-small and small cell lung cancer. \textit{Colloids and Surfaces B: Biointerfaces}. Amsterdam: Elsevier, 2017, roč.~150, 1 February 2017, s.~131-140. ISSN~0927-7765. Dostupné z: https://doi.org/10.1016/j.colsurfb.2016.11.023.

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