Capillary Isoelectric Focusing-Useful Tool for Detection and
Quantification of Lactic Acid Bacteria in Milk

J 2016

Capillary Isoelectric Focusing-Useful Tool for Detection and Quantification of Lactic Acid Bacteria in Milk

RŮŽIČKA, Filip; M. HORKA; Veronika HOLÁ; Katarína REBROŠOVÁ; V. DRAB et al.

Základní údaje

Originální název

Capillary Isoelectric Focusing-Useful Tool for Detection and Quantification of Lactic Acid Bacteria in Milk

Autoři

RŮŽIČKA, Filip ; M. HORKA; Veronika HOLÁ ; Katarína REBROŠOVÁ a V. DRAB

Vydání

Food Analytical Methods, New York, Springer, 2016, 1936-9751

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.038

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/16:00088929

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

Lactic acid bacteria; Lactobacillus; Enterococcus; Capillary isoelectric focusing; Milk; Isoelectric point; pI

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 12. 2016 14:19, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

The lactic acid bacteria (LAB), including lactobacilli and enterococci, represent an important part of normal microflora in humans. Simultaneously, they are frequently used as probiotics and in food industry for production of fermented milk products. The rapid and sensitive detection of these microorganisms is crucial for the quality control. In this study, the capillary isoelectric focusing (CIEF) was successfully used for the separation and characterization of probiotic LAB, L. paracasei sp. paracasei; L. acidifarinae; L. fermentum; L. gasseri; L. helveticus; L. plantarum; L. delbrueckii; L. salivarius; E. durans; E. faecalis; and E. faecium, according to their isoelectric points (pI). All pIs of lactobacilli and enterococci were found in the acidic part of the pH range. Subsequently, the milk samples spiked with L. rhamnosus, and E. faecium were analyzed by CIEF. The optimal adjustment of the separation conditions allowed detection and quantification of the bacteria in the sample with sufficient sensitivity. Therefore, CIEF is an efficient approach to rapid detection and separation of LAB even directly in milk products in future.

Návaznosti

NV16-29916A, projekt VaV

Název: Využití bakteriofágů v léčbě nozokomiálních infekcí spojených s multirezistencí či tvorbou biofilmu

NV16-31593A, projekt VaV

Název: Polymikrobiální biofilm jako komplexní mikrobiom v rámci léčby nozokomiálních infekcí

Citovat

RŮŽIČKA, Filip; M. HORKA; Veronika HOLÁ; Katarína REBROŠOVÁ a V. DRAB. Capillary Isoelectric Focusing-Useful Tool for Detection and Quantification of Lactic Acid Bacteria in Milk. Food Analytical Methods. New York: Springer, 2016, roč. 9, č. 12, s. 3251-3257. ISSN 1936-9751. Dostupné z: https://doi.org/10.1007/s12161-016-0522-6.

@article{1365011,
   author = {Růžička, Filip and Horka, M. and Holá, Veronika and Rebrošová, Katarína and Drab, V.},
   article_location = {New York},
   article_number = {12},
   doi = {https://doi.org/10.1007/s12161-016-0522-6},
   keywords = {Lactic acid bacteria; Lactobacillus; Enterococcus; Capillary isoelectric focusing; Milk; Isoelectric point; pI},
   language = {eng},
   issn = {1936-9751},
   journal = {Food Analytical Methods},
   title = {Capillary Isoelectric Focusing-Useful Tool for Detection and Quantification of Lactic Acid Bacteria in Milk},
   volume = {9},
   year = {2016}
}

TY  - JOUR
ID  - 1365011
AU  - Růžička, Filip - Horka, M. - Holá, Veronika - Rebrošová, Katarína - Drab, V.
PY  - 2016
TI  - Capillary Isoelectric Focusing-Useful Tool for Detection and Quantification of Lactic Acid Bacteria in Milk
JF  - Food Analytical Methods
VL  - 9
IS  - 12
SP  - 3251-3257
EP  - 3251-3257
PB  - Springer
SN  - 19369751
KW  - Lactic acid bacteria
KW  - Lactobacillus
KW  - Enterococcus
KW  - Capillary isoelectric focusing
KW  - Milk
KW  - Isoelectric point
KW  - pI
N2  - The lactic acid bacteria (LAB), including lactobacilli and enterococci, represent an important part of normal microflora in humans. Simultaneously, they are frequently used as probiotics and in food industry for production of fermented milk products. The rapid and sensitive detection of these microorganisms is crucial for the quality control. In this study, the capillary isoelectric focusing (CIEF) was successfully used for the separation and characterization of probiotic LAB, L. paracasei sp. paracasei; L. acidifarinae; L. fermentum; L. gasseri; L. helveticus; L. plantarum; L. delbrueckii; L. salivarius; E. durans; E. faecalis; and E. faecium, according to their isoelectric points (pI). All pIs of lactobacilli and enterococci were found in the acidic part of the pH range. Subsequently, the milk samples spiked with L. rhamnosus, and E. faecium were analyzed by CIEF. The optimal adjustment of the separation conditions allowed detection and quantification of the bacteria in the sample with sufficient sensitivity. Therefore, CIEF is an efficient approach to rapid detection and separation of LAB even directly in milk products in future.
ER  -

RŮŽIČKA, Filip; M. HORKA; Veronika HOLÁ; Katarína REBROŠOVÁ a V. DRAB. Capillary Isoelectric Focusing-Useful Tool for Detection and Quantification of Lactic Acid Bacteria in Milk. \textit{Food Analytical Methods}. New York: Springer, 2016, roč.~9, č.~12, s.~3251-3257. ISSN~1936-9751. Dostupné z: https://doi.org/10.1007/s12161-016-0522-6.

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