Capillary electrophoresis in a fused-silica capillary with
surface roughness gradient

J 2016

Capillary electrophoresis in a fused-silica capillary with surface roughness gradient

HORKÁ, Marie; Karel ŠLAIS; Pavel KARÁSEK; Filip RŮŽIČKA; Jiří ŠALPLACHTA et al.

Základní údaje

Originální název

Capillary electrophoresis in a fused-silica capillary with surface roughness gradient

Autoři

HORKÁ, Marie; Karel ŠLAIS; Pavel KARÁSEK; Filip RŮŽIČKA ; Jiří ŠALPLACHTA; Jozef ŠESTÁK; Vladislav KAHLE a Michal ROTH

Vydání

Journal of Separation Science, Weinheim, Wiley-VCH Verlag, 2016, 1615-9306

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Německo

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.557

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/16:00088930

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

Capillary electrophoresis; Fused-silica capillaries; Supercritical water; Surface roughness gradient

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 26. 4. 2017 12:27, Soňa Böhmová

Anotace

V originále

The electro-osmotic flow, a significant factor in capillary electrophoretic separations, is very sensitive to small changes in structure and surface roughness of the inner surface of fused silica capillary. Besides a number of negative effects, the electro-osmotic flow can also have a positive effect on the separation. An example could be fused silica capillaries with homogenous surface roughness along their entire separation length as produced by etching with supercritical water. Different strains of methicillin-resistant and methicillin-susceptible Staphylococcus aureus were separated on that type of capillaries. In the present study, fused-silica capillaries with a gradient of surface roughness were prepared and their basic behavior was studied in capillary zone electrophoresis with UV-visible detection. First the influence of the electro-osmotic flow on the peak shape of a marker of electro-osmotic flow, thiourea, has been discussed. An antifungal agent, hydrophobic amphotericin B, and a protein marker, albumin, have been used as model analytes. A significant narrowing of the detected zones of the examined analytes was achieved in supercritical-water-treated capillaries as compared to the electrophoretic separation in smooth capillaries. Minimum detectable amounts of 5 ng/mL amphotericin B and 5 microg/mL albumin were reached with this method.

Návaznosti

NV16-29916A, projekt VaV

Název: Využití bakteriofágů v léčbě nozokomiálních infekcí spojených s multirezistencí či tvorbou biofilmu

Citovat

HORKÁ, Marie; Karel ŠLAIS; Pavel KARÁSEK; Filip RŮŽIČKA; Jiří ŠALPLACHTA; Jozef ŠESTÁK; Vladislav KAHLE a Michal ROTH. Capillary electrophoresis in a fused-silica capillary with surface roughness gradient. Journal of Separation Science. Weinheim: Wiley-VCH Verlag, 2016, roč. 39, č. 19, s. 3827-3834. ISSN 1615-9306. Dostupné z: https://doi.org/10.1002/jssc.201600660.

@article{1365071,
   author = {Horká, Marie and Šlais, Karel and Karásek, Pavel and Růžička, Filip and Šalplachta, Jiří and Šesták, Jozef and Kahle, Vladislav and Roth, Michal},
   article_location = {Weinheim},
   article_number = {19},
   doi = {https://doi.org/10.1002/jssc.201600660},
   keywords = {Capillary electrophoresis; Fused-silica capillaries; Supercritical water; Surface roughness gradient},
   language = {eng},
   issn = {1615-9306},
   journal = {Journal of Separation Science},
   title = {Capillary electrophoresis in a fused-silica capillary with surface roughness gradient},
   volume = {39},
   year = {2016}
}

TY  - JOUR
ID  - 1365071
AU  - Horká, Marie - Šlais, Karel - Karásek, Pavel - Růžička, Filip - Šalplachta, Jiří - Šesták, Jozef - Kahle, Vladislav - Roth, Michal
PY  - 2016
TI  - Capillary electrophoresis in a fused-silica capillary with surface roughness gradient
JF  - Journal of Separation Science
VL  - 39
IS  - 19
SP  - 3827-3834
EP  - 3827-3834
PB  - Wiley-VCH Verlag
SN  - 16159306
KW  - Capillary electrophoresis
KW  - Fused-silica capillaries
KW  - Supercritical water
KW  - Surface roughness gradient
N2  - The electro-osmotic flow, a significant factor in capillary electrophoretic separations, is very sensitive to small changes in structure and surface roughness of the inner surface of fused silica capillary. Besides a number of negative effects, the electro-osmotic flow can also have a positive effect on the separation. An example could be fused silica capillaries with homogenous surface roughness along their entire separation length as produced by etching with supercritical water. Different strains of methicillin-resistant and methicillin-susceptible Staphylococcus aureus were separated on that type of capillaries. In the present study, fused-silica capillaries with a gradient of surface roughness were prepared and their basic behavior was studied in capillary zone electrophoresis with UV-visible detection. First the influence of the electro-osmotic flow on the peak shape of a marker of electro-osmotic flow, thiourea, has been discussed. An antifungal agent, hydrophobic amphotericin B, and a protein marker, albumin, have been used as model analytes. A significant narrowing of the detected zones of the examined analytes was achieved in supercritical-water-treated capillaries as compared to the electrophoretic separation in smooth capillaries. Minimum detectable amounts of 5 ng/mL amphotericin B and 5 microg/mL albumin were reached with this method.
ER  -

HORKÁ, Marie; Karel ŠLAIS; Pavel KARÁSEK; Filip RŮŽIČKA; Jiří ŠALPLACHTA; Jozef ŠESTÁK; Vladislav KAHLE a Michal ROTH. Capillary electrophoresis in a fused-silica capillary with surface roughness gradient. \textit{Journal of Separation Science}. Weinheim: Wiley-VCH Verlag, 2016, roč.~39, č.~19, s.~3827-3834. ISSN~1615-9306. Dostupné z: https://doi.org/10.1002/jssc.201600660.

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