The crucial role of emilin 1 gene expression during progression
of tumor growth

J 2016

The crucial role of emilin 1 gene expression during progression of tumor growth

RABAJDOVA, Miroslava; Peter URBAN; Ivana SPAKOVA; Ladislav SAKSUN; Rastislav DUDIC et al.

Základní údaje

Originální název

The crucial role of emilin 1 gene expression during progression of tumor growth

Autoři

RABAJDOVA, Miroslava; Peter URBAN; Ivana SPAKOVA; Ladislav SAKSUN; Rastislav DUDIC; Alexander OSTRO; Martin CAPRNDA; Peter KRUŽLIAK; Mariusz ADAMEK a Maria MAREKOVA

Vydání

Journal of cancer research and clinical oncology, Germany, Springer-Verlag, 2016, 0171-5216

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.503

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/16:00092422

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

Breast cancer; Tumor microenvironment; Gene expression; Hypoxia; Metastases

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 2. 2. 2017 12:16, Ing. Mgr. Věra Pospíšilíková

Anotace

V originále

This study describes the effect of rapid tumor growth of patients suffering from various grades of malignant ductal breast carcinoma associated with the gene expression of ECM protein emilin 1, in correlation with the number of gene copies of emilin 1 and degradation of tumor tissue proteins. A total of 40 examined patients participated in the experiment (controls, n = 10, grades GI-GIII, each n = 10). After isolation of total mRNA, transcription of mRNA into the cDNA was performed. Quantification of gene expression changes was detected by the real-time PCR method. Analysis at the protein level was performed via Western blot method. During the detection of changes at the mRNA level, a significantly decreased level of emilin 1 in tumor tissues with grade II (about 54 +/- A 8 % lower than control) was identified. Protein-level analysis indicated an increased level of emilin 1 in tumors with grade I in comparison with control samples (about 10 +/- A 3 %). Obtained results demonstrated that the suppressive role of emilin 1 is related to the grade of growing breast tumors, and associated with increased hypoxia in the tumor microenvironment followed by elevated unfolding and degradation of tissue proteins.

Citovat

RABAJDOVA, Miroslava; Peter URBAN; Ivana SPAKOVA; Ladislav SAKSUN; Rastislav DUDIC; Alexander OSTRO; Martin CAPRNDA; Peter KRUŽLIAK; Mariusz ADAMEK a Maria MAREKOVA. The crucial role of emilin 1 gene expression during progression of tumor growth. Journal of cancer research and clinical oncology. Germany: Springer-Verlag, 2016, roč. 142, č. 11, s. 2397-2402. ISSN 0171-5216. Dostupné z: https://doi.org/10.1007/s00432-016-2226-0.

@article{1365457,
   author = {Rabajdova, Miroslava and Urban, Peter and Spakova, Ivana and Saksun, Ladislav and Dudic, Rastislav and Ostro, Alexander and Caprnda, Martin and Kružliak, Peter and Adamek, Mariusz and Marekova, Maria},
   article_location = {Germany},
   article_number = {11},
   doi = {https://doi.org/10.1007/s00432-016-2226-0},
   keywords = {Breast cancer; Tumor microenvironment; Gene expression; Hypoxia; Metastases},
   language = {eng},
   issn = {0171-5216},
   journal = {Journal of cancer research and clinical oncology},
   title = {The crucial role of emilin 1 gene expression during progression of tumor growth},
   volume = {142},
   year = {2016}
}

TY  - JOUR
ID  - 1365457
AU  - Rabajdova, Miroslava - Urban, Peter - Spakova, Ivana - Saksun, Ladislav - Dudic, Rastislav - Ostro, Alexander - Caprnda, Martin - Kružliak, Peter - Adamek, Mariusz - Marekova, Maria
PY  - 2016
TI  - The crucial role of emilin 1 gene expression during progression of tumor growth
JF  - Journal of cancer research and clinical oncology
VL  - 142
IS  - 11
SP  - 2397-2402
EP  - 2397-2402
PB  - Springer-Verlag
SN  - 01715216
KW  - Breast cancer
KW  - Tumor microenvironment
KW  - Gene expression
KW  - Hypoxia
KW  - Metastases
N2  - This study describes the effect of rapid tumor growth of patients suffering from various grades of malignant ductal breast carcinoma associated with the gene expression of ECM protein emilin 1, in correlation with the number of gene copies of emilin 1 and degradation of tumor tissue proteins. A total of 40 examined patients participated in the experiment (controls, n = 10, grades GI-GIII, each n = 10). After isolation of total mRNA, transcription of mRNA into the cDNA was performed. Quantification of gene expression changes was detected by the real-time PCR method. Analysis at the protein level was performed via Western blot method. During the detection of changes at the mRNA level, a significantly decreased level of emilin 1 in tumor tissues with grade II (about 54 +/- A 8 % lower than control) was identified. Protein-level analysis indicated an increased level of emilin 1 in tumors with grade I in comparison with control samples (about 10 +/- A 3 %). Obtained results demonstrated that the suppressive role of emilin 1 is related to the grade of growing breast tumors, and associated with increased hypoxia in the tumor microenvironment followed by elevated unfolding and degradation of tissue proteins.
ER  -

RABAJDOVA, Miroslava; Peter URBAN; Ivana SPAKOVA; Ladislav SAKSUN; Rastislav DUDIC; Alexander OSTRO; Martin CAPRNDA; Peter KRUŽLIAK; Mariusz ADAMEK a Maria MAREKOVA. The crucial role of emilin 1 gene expression during progression of tumor growth. \textit{Journal of cancer research and clinical oncology}. Germany: Springer-Verlag, 2016, roč.~142, č.~11, s.~2397-2402. ISSN~0171-5216. Dostupné z: https://doi.org/10.1007/s00432-016-2226-0.

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