Další formáty:
BibTeX
LaTeX
RIS
@article{1366238, author = {Cmiel, Vratislav and Skopalik, Josef and Polakova, Katerina and Solar, Jan and Havrdova, Marketa and Milde, David and Justan, Ivan and Magro, Masimiliano and Starcuk, Zenon and Provaznik, Ivo}, article_location = {New York}, article_number = {5}, doi = {http://dx.doi.org/10.1007/s00249-016-1187-1}, keywords = {Confocal microscopy; Dual contrast agents; Intracellular fluorescent labels; Iron oxide nanoparticles; Mesenchymal stromal cells; Rhodamine; Stem cell tracking}, language = {eng}, issn = {0175-7571}, journal = {European Biophysics Journal}, title = {Rhodamine bound maghemite as a long-term dual imaging nanoprobe of adipose tissue-derived mesenchymal stromal cells}, url = {http://dx.doi.org/10.1007/s00249-016-1187-1}, volume = {46}, year = {2016} }
TY - JOUR ID - 1366238 AU - Cmiel, Vratislav - Skopalik, Josef - Polakova, Katerina - Solar, Jan - Havrdova, Marketa - Milde, David - Justan, Ivan - Magro, Masimiliano - Starcuk, Zenon - Provaznik, Ivo PY - 2016 TI - Rhodamine bound maghemite as a long-term dual imaging nanoprobe of adipose tissue-derived mesenchymal stromal cells JF - European Biophysics Journal VL - 46 IS - 5 SP - 433-444 EP - 433-444 PB - Springer SN - 01757571 KW - Confocal microscopy KW - Dual contrast agents KW - Intracellular fluorescent labels KW - Iron oxide nanoparticles KW - Mesenchymal stromal cells KW - Rhodamine KW - Stem cell tracking UR - http://dx.doi.org/10.1007/s00249-016-1187-1 L2 - http://dx.doi.org/10.1007/s00249-016-1187-1 N2 - In the last few years, magnetically labeled cells have been intensively explored, and non-invasive cell tracking and magnetic manipulation methods have been tested in preclinical studies focused on cell transplantation. For clinical applications, it is desirable to know the intracellular pathway of nanoparticles, which can predict their biocompatibility with cells and the long-term imaging properties of labeled cells. Here, we quantified labeling efficiency, localization, and fluorescence properties of Rhodamine derivatized superparamagnetic maghemite nanoparticles (SAMN-R) in mesenchymal stromal cells (MSC). We investigated the stability of SAMN-R in the intracellular space during a long culture (20 days). Analyses were based on advanced confocal microscopy accompanied by atomic absorption spectroscopy (AAS) and magnetic resonance imaging. SAMN-R displayed excellent cellular uptake (24 h of labeling), and no toxicity of SAMN-R labeling was found. 83% of SAMN-R nanoparticles were localized in lysosomes, only 4.8% were found in mitochondria, and no particles were localized in the nucleus. On the basis of the MSC fluorescence measurement every 6 days, we also quantified the continual decrease of SAMN-R fluorescence in the average single MSC during 18 days. An additional set of analyses showed that the intracellular SAMN-R signal decrease was minimally caused by fluorophore degradation or nanoparticles extraction from the cells, main reason is a cell division. The fluorescence of SAMN-R nanoparticles within the cells was detectable minimally for 20 days. These observations indicate that SAMN-R nanoparticles have a potential for application in transplantation medicine. ER -
CMIEL, Vratislav, Josef SKOPALIK, Katerina POLAKOVA, Jan SOLAR, Marketa HAVRDOVA, David MILDE, Ivan JUSTAN, Masimiliano MAGRO, Zenon STARCUK a Ivo PROVAZNIK. Rhodamine bound maghemite as a long-term dual imaging nanoprobe of adipose tissue-derived mesenchymal stromal cells. \textit{European Biophysics Journal}. New York: Springer, 2016, roč.~46, č.~5, s.~433-444. ISSN~0175-7571. Dostupné z: https://dx.doi.org/10.1007/s00249-016-1187-1.
|