PRDM9 interactions with other proteins provide a link between
recombination hotspots and the chromosomal axis in meiosis

J 2017

PRDM9 interactions with other proteins provide a link between recombination hotspots and the chromosomal axis in meiosis

PARVANOV, Emil Damyanov; Hui TIAN; Timothy BILLINGS; Ruth L. SAXL; Catrina SPRUCE et. al.

Základní údaje

Originální název

PRDM9 interactions with other proteins provide a link between recombination hotspots and the chromosomal axis in meiosis

Autoři

PARVANOV, Emil Damyanov; Hui TIAN; Timothy BILLINGS; Ruth L. SAXL; Catrina SPRUCE; Rakesh AITHAL; Lumír KREJČÍ; Kenneth PAIGEN a Petko M. PETKOV

Vydání

Molecular Biology of the Cell, Bethesda, American Society for Cell Biology, 2017, 1059-1524

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10601 Cell biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.512

Kód RIV

RIV/00216224:14110/17:00094599

Organizační jednotka

Lékařská fakulta

DOI

https://doi.org/10.1091/mbc.E16-09-0686

UT WoS

000394268700014

EID Scopus

2-s2.0-85011660720

Klíčová slova anglicky

CXXC FINGER PROTEIN-1; MEIOTIC RECOMBINATION; HISTONE METHYLTRANSFERASES; CHROMATIN MODIFICATIONS; BIOLOGICAL FUNCTIONS; GENE REARRANGEMENTS; ANALYSIS REVEALS; EWING SARCOMA; HOT-SPOTS; METHYLATION

Štítky

EL OK, podil

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 18. 3. 2018 15:40, Soňa Böhmová

Anotace

V originále

In mammals, meiotic recombination occurs at 1-2 kb genomic regions termed hotspots, whose positions and activities are determined by PRDM9, a DNA-binding histone methyltransferase. We now show that the KRAB domain of PRDM9 forms complexes with additional proteins to allow hotspots to proceed into the next phase of recombination. By a combination of yeast-two hybrid assay, in vitro binding, and co-immunoprecipitation from mouse spermatocytes, we identified four proteins that directly interact with PRDM9's KRAB domain, namely CXXC1, EWSR1, EHMT2, and CDYL. These proteins are co-expressed in spermatocytes at the early stages of meiotic prophase I, the limited period when PRDM9 is expressed. We also detected association of PRDM9-bound complexes with the meiotic cohesin REC8 and the synaptonemal complex proteins SYCP3 and SYCP1. Our results suggest a model in which PRDM9-bound hotspot DNA is brought to the chromosomal axis by the action of these proteins, ensuring the proper chromatin and spatial environment for subsequent recombination events.

Návaznosti

GAP207/12/2323, projekt VaV

Název: Endonuleazová a translokázová aktivita v restričních-modifikáčních komplexéch typu I

Investor: Grantová agentura ČR, Endonuleázová a translokázová aktivita v restrikčních-modifikačních komplexech typu I

GA13-26629S, projekt VaV

Název: SUMO a stability genomu

Investor: Grantová agentura ČR, SUMO a stability genomu

2SGA2773, interní kód MU

Název: Prdm9 - linking the histone modifications and recombination (Akronym: PLHMR)

Investor: Jihomoravský kraj, Prdm9 - linking the histone modifications and recombination, Granty pro zahraniční vědce

Citovat

PARVANOV, Emil Damyanov; Hui TIAN; Timothy BILLINGS; Ruth L. SAXL; Catrina SPRUCE; Rakesh AITHAL; Lumír KREJČÍ; Kenneth PAIGEN a Petko M. PETKOV. PRDM9 interactions with other proteins provide a link between recombination hotspots and the chromosomal axis in meiosis. Molecular Biology of the Cell. Bethesda: American Society for Cell Biology, 2017, roč. 28, č. 3, s. 488-499. ISSN 1059-1524. Dostupné z: https://doi.org/10.1091/mbc.E16-09-0686.

@article{1367105,
   author = {Parvanov, Emil Damyanov and Tian, Hui and Billings, Timothy and Saxl, Ruth L. and Spruce, Catrina and Aithal, Rakesh and Krejčí, Lumír and Paigen, Kenneth and Petkov, Petko M.},
   article_location = {Bethesda},
   article_number = {3},
   doi = {https://doi.org/10.1091/mbc.E16-09-0686},
   keywords = {CXXC FINGER PROTEIN-1; MEIOTIC RECOMBINATION; HISTONE METHYLTRANSFERASES; CHROMATIN MODIFICATIONS; BIOLOGICAL FUNCTIONS; GENE REARRANGEMENTS; ANALYSIS REVEALS; EWING SARCOMA; HOT-SPOTS; METHYLATION},
   language = {eng},
   issn = {1059-1524},
   journal = {Molecular Biology of the Cell},
   title = {PRDM9 interactions with other proteins provide a link between recombination hotspots and the chromosomal axis in meiosis},
   volume = {28},
   year = {2017}
}

TY  - JOUR
ID  - 1367105
AU  - Parvanov, Emil Damyanov - Tian, Hui - Billings, Timothy - Saxl, Ruth L. - Spruce, Catrina - Aithal, Rakesh - Krejčí, Lumír - Paigen, Kenneth - Petkov, Petko M.
PY  - 2017
TI  - PRDM9 interactions with other proteins provide a link between recombination hotspots and the chromosomal axis in meiosis
JF  - Molecular Biology of the Cell
VL  - 28
IS  - 3
SP  - 488-499
EP  - 488-499
PB  - American Society for Cell Biology
SN  - 10591524
KW  - CXXC FINGER PROTEIN-1
KW  - MEIOTIC RECOMBINATION
KW  - HISTONE METHYLTRANSFERASES
KW  - CHROMATIN MODIFICATIONS
KW  - BIOLOGICAL FUNCTIONS
KW  - GENE REARRANGEMENTS
KW  - ANALYSIS REVEALS
KW  - EWING SARCOMA
KW  - HOT-SPOTS
KW  - METHYLATION
N2  - In mammals, meiotic recombination occurs at 1-2 kb genomic regions termed hotspots, whose positions and activities are determined by PRDM9, a DNA-binding histone methyltransferase. We now show that the KRAB domain of PRDM9 forms complexes with additional proteins to allow hotspots to proceed into the next phase of recombination. By a combination of yeast-two hybrid assay, in vitro binding, and co-immunoprecipitation from mouse spermatocytes, we identified four proteins that directly interact with PRDM9's KRAB domain, namely CXXC1, EWSR1, EHMT2, and CDYL. These proteins are co-expressed in spermatocytes at the early stages of meiotic prophase I, the limited period when PRDM9 is expressed. We also detected association of PRDM9-bound complexes with the meiotic cohesin REC8 and the synaptonemal complex proteins SYCP3 and SYCP1. Our results suggest a model in which PRDM9-bound hotspot DNA is brought to the chromosomal axis by the action of these proteins, ensuring the proper chromatin and spatial environment for subsequent recombination events.
ER  -

PARVANOV, Emil Damyanov; Hui TIAN; Timothy BILLINGS; Ruth L. SAXL; Catrina SPRUCE; Rakesh AITHAL; Lumír KREJČÍ; Kenneth PAIGEN a Petko M. PETKOV. PRDM9 interactions with other proteins provide a link between recombination hotspots and the chromosomal axis in meiosis. \textit{Molecular Biology of the Cell}. Bethesda: American Society for Cell Biology, 2017, roč.~28, č.~3, s.~488-499. ISSN~1059-1524. Dostupné z: https://doi.org/10.1091/mbc.E16-09-0686.

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