Enquiry into the Topology of Plasma Membrane-Localized PIN
Auxin Transport Components

J 2016

Enquiry into the Topology of Plasma Membrane-Localized PIN Auxin Transport Components

NODZYNSKI, Tomasz, Steffen VANNESTE, Marta ZWIEWKA, Markéta PERNISOVÁ, Jan HEJÁTKO et. al.

Základní údaje

Originální název

Enquiry into the Topology of Plasma Membrane-Localized PIN Auxin Transport Components

Autoři

NODZYNSKI, Tomasz (616 Polsko, garant, domácí), Steffen VANNESTE (56 Belgie), Marta ZWIEWKA (616 Polsko, domácí), Markéta PERNISOVÁ (203 Česká republika, domácí), Jan HEJÁTKO (203 Česká republika, domácí) a Jiří FRIML (203 Česká republika)

Vydání

Molecular Plant, CAMBRIDGE, Cell Press, 2016, 1674-2052

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 8.827

Kód RIV

RIV/00216224:14740/16:00088530

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1016/j.molp.2016.08.010

UT WoS

000389594100008

Klíčová slova anglicky

plasma membrane protein; topology; auxin efflux carriers; Arabidopsis thaliana

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 3. 2018 22:42, Mgr. Markéta Pernisová, Ph.D.

Anotace

V originále

Auxin directs plant ontogenesis via differential accumulation within tissues depending largely on the activity of PIN proteins that mediate auxin efflux from cells and its directional cell-to-cell transport. Regardless of the developmental importance of PINs, the structure of these transporters is poorly characterized. Here, we present experimental data concerning protein topology of plasma membrane-localized PINs. Utilizing approaches based on pH-dependent quenching of fluorescent reporters combined with immunolocalization techniques,we mapped the membrane topology of PINs and further cross-validated our results using available topology modeling software. We delineated the topology of PIN1 with two transmembrane (TM) bundles of five a-helices linked by a large intracellular loop and a C-terminus positioned outside the cytoplasm. Using constraints derived from our experimental data, we also provide an updated position of helical regions generating a verisimilitude model of PIN1. Since the canonical long PINs show a high degree of conservation in TM domains and auxin transport capacity has been demonstrated for Arabidopsis representatives of this group, this empirically enhanced topological model of PIN1 will be an important starting point for further studies on PIN structure-function relationships. In addition, we have established protocols that can be used to probe the topology of other plasma membrane proteins in plants.

Návaznosti

GA13-39982S, projekt VaV

Název: Molekulární mechanismy auxín-cytokinínové interakce v regulaci rostlinné organogenese.

Investor: Grantová agentura ČR, Molekulární mechanismy auxín-cytokinínové interakce v regulaci rostlinné organogenese

GA13-40637S, projekt VaV

Název: Genetické studie k identifikaci molekulárních mechanizmů buněčné polarity a auxinového transportu v rostlinách

Investor: Grantová agentura ČR, Genetické studie k identifikaci molekulárních mechanizmu bunecné polarity a auxinového transportu v rostlinách

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

NODZYNSKI, Tomasz, Steffen VANNESTE, Marta ZWIEWKA, Markéta PERNISOVÁ, Jan HEJÁTKO a Jiří FRIML. Enquiry into the Topology of Plasma Membrane-Localized PIN Auxin Transport Components. Molecular Plant. CAMBRIDGE: Cell Press, 2016, roč. 9, č. 11, s. 1504-1519. ISSN 1674-2052. Dostupné z: https://dx.doi.org/10.1016/j.molp.2016.08.010.

@article{1367181,
   author = {Nodzynski, Tomasz and Vanneste, Steffen and Zwiewka, Marta and Pernisová, Markéta and Hejátko, Jan and Friml, Jiří},
   article_location = {CAMBRIDGE},
   article_number = {11},
   doi = {http://dx.doi.org/10.1016/j.molp.2016.08.010},
   keywords = {plasma membrane protein; topology; auxin efflux carriers; Arabidopsis thaliana},
   language = {eng},
   issn = {1674-2052},
   journal = {Molecular Plant},
   title = {Enquiry into the Topology of Plasma Membrane-Localized PIN Auxin Transport Components},
   url = {http://www.cell.com/molecular-plant/fulltext/S1674-2052(16)30191-5},
   volume = {9},
   year = {2016}
}

TY  - JOUR
ID  - 1367181
AU  - Nodzynski, Tomasz - Vanneste, Steffen - Zwiewka, Marta - Pernisová, Markéta - Hejátko, Jan - Friml, Jiří
PY  - 2016
TI  - Enquiry into the Topology of Plasma Membrane-Localized PIN Auxin Transport Components
JF  - Molecular Plant
VL  - 9
IS  - 11
SP  - 1504-1519
EP  - 1504-1519
PB  - Cell Press
SN  - 16742052
KW  - plasma membrane protein
KW  - topology
KW  - auxin efflux carriers
KW  - Arabidopsis thaliana
UR  - http://www.cell.com/molecular-plant/fulltext/S1674-2052(16)30191-5
L2  - http://www.cell.com/molecular-plant/fulltext/S1674-2052(16)30191-5
N2  - Auxin directs plant ontogenesis via differential accumulation within tissues depending largely on the activity of PIN proteins that mediate auxin efflux from cells and its directional cell-to-cell transport. Regardless of the developmental importance of PINs, the structure of these transporters is poorly characterized. Here, we present experimental data concerning protein topology of plasma membrane-localized PINs. Utilizing approaches based on pH-dependent quenching of fluorescent reporters combined with immunolocalization techniques,we mapped the membrane topology of PINs and further cross-validated our results using available topology modeling software. We delineated the topology of PIN1 with two transmembrane (TM) bundles of five a-helices linked by a large intracellular loop and a C-terminus positioned outside the cytoplasm. Using constraints derived from our experimental data, we also provide an updated position of helical regions generating a verisimilitude model of PIN1. Since the canonical long PINs show a high degree of conservation in TM domains and auxin transport capacity has been demonstrated for Arabidopsis representatives of this group, this empirically enhanced topological model of PIN1 will be an important starting point for further studies on PIN structure-function relationships. In addition, we have established protocols that can be used to probe the topology of other plasma membrane proteins in plants.
ER  -

NODZYNSKI, Tomasz, Steffen VANNESTE, Marta ZWIEWKA, Markéta PERNISOVÁ, Jan HEJÁTKO a Jiří FRIML. Enquiry into the Topology of Plasma Membrane-Localized PIN Auxin Transport Components. \textit{Molecular Plant}. CAMBRIDGE: Cell Press, 2016, roč.~9, č.~11, s.~1504-1519. ISSN~1674-2052. Dostupné z: https://dx.doi.org/10.1016/j.molp.2016.08.010.

Podrobný výpis o publikaci