On the molecular pharmacology of resveratrol on oxidative burst inhibition in professional phagocytes

NOSÁL, Radomír; Katarína DRÁBIKOVÁ; Viera JANČINOVÁ; Tomáš PEREČKO; Gabriela AMBROŽOVÁ; Milan ČÍŽ; Antonín LOJEK; Michaela PEKAROVÁ; Jan ŠMIDRKAL a Juraj HARMATHA. On the molecular pharmacology of resveratrol on oxidative burst inhibition in professional phagocytes. Oxidative Medicine and Cellular Longevity. New York: Hindawi Publishing Corporation, 2014. ISSN 1942-0900. Dostupné z: https://doi.org/10.1155/2014/706269.

Základní údaje

Originální název

On the molecular pharmacology of resveratrol on oxidative burst inhibition in professional phagocytes

Název česky

On the molecular pharmacology of resveratrol on oxidative burst inhibition in professional phagocytes

Název anglicky

On the molecular pharmacology of resveratrol on oxidative burst inhibition in professional phagocytes

Autoři

NOSÁL, Radomír; Katarína DRÁBIKOVÁ; Viera JANČINOVÁ; Tomáš PEREČKO; Gabriela AMBROŽOVÁ; Milan ČÍŽ; Antonín LOJEK; Michaela PEKAROVÁ; Jan ŠMIDRKAL a Juraj HARMATHA

Vydání

Oxidative Medicine and Cellular Longevity, New York, Hindawi Publishing Corporation, 2014, 1942-0900

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Další údaje

Typ výsledku

Článek v odborném periodiku

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.516

Označené pro přenos do RIV

Ne

DOI

https://doi.org/10.1155/2014/706269

Klíčová slova česky

Resveratrol, Oxidative Burst, Professional Phagocytes

Klíčová slova anglicky

Resveratrol, Oxidative Burst, Professional Phagocytes

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Anotace

V originále

Resveratrol—3,5,4′-trihydroxystilbene—possesses antioxidant activities in vitro. It dose-dependently inhibited the generation of peroxyl, hydroxyl, peroxides, and lipid peroxidation products in cell free systems. Oxidative burst of whole human blood stimulated with PMA, fMLP, OpZ, and A23187 was inhibited in a concentration-dependent way, indicating suppression of both receptor and nonreceptor activated chemiluminescence by resveratrol. Results from isolated human neutrophils revealed that resveratrol was active extracellularly as well as intracellularly in inhibiting the generation of reactive oxygen species. Liberation of ATP and analysis of apoptosis showed that in the concentration of 100 μM, resveratrol did not change the viability and integrity of isolated neutrophils. Western blot analysis documented that resveratrol in concentrations of 10 and 100 μM significantly decreased PMA-induced phosphorylation of PKC α/βII. Dose-dependent inhibition of nitrite production and iNOS protein expression in RAW 264.7 cells indicated possible interference of resveratrol with reactive nitrogen radical generation in professional phagocytes. The results suggest that resveratrol represents an effective naturally occurring substance with potent pharmacological effect on oxidative burst of human neutrophils and nitric oxide production by macrophages. It should be further investigated for its pharmacological activity against oxidative stress in ischaemia reperfusion, inflammation, and other pathological conditions, particularly neoplasia.

Česky

Resveratrol—3,5,4′-trihydroxystilbene—possesses antioxidant activities in vitro. It dose-dependently inhibited the generation of peroxyl, hydroxyl, peroxides, and lipid peroxidation products in cell free systems. Oxidative burst of whole human blood stimulated with PMA, fMLP, OpZ, and A23187 was inhibited in a concentration-dependent way, indicating suppression of both receptor and nonreceptor activated chemiluminescence by resveratrol. Results from isolated human neutrophils revealed that resveratrol was active extracellularly as well as intracellularly in inhibiting the generation of reactive oxygen species. Liberation of ATP and analysis of apoptosis showed that in the concentration of 100 μM, resveratrol did not change the viability and integrity of isolated neutrophils. Western blot analysis documented that resveratrol in concentrations of 10 and 100 μM significantly decreased PMA-induced phosphorylation of PKC α/βII. Dose-dependent inhibition of nitrite production and iNOS protein expression in RAW 264.7 cells indicated possible interference of resveratrol with reactive nitrogen radical generation in professional phagocytes. The results suggest that resveratrol represents an effective naturally occurring substance with potent pharmacological effect on oxidative burst of human neutrophils and nitric oxide production by macrophages. It should be further investigated for its pharmacological activity against oxidative stress in ischaemia reperfusion, inflammation, and other pathological conditions, particularly neoplasia.

Anglicky

Resveratrol—3,5,4′-trihydroxystilbene—possesses antioxidant activities in vitro. It dose-dependently inhibited the generation of peroxyl, hydroxyl, peroxides, and lipid peroxidation products in cell free systems. Oxidative burst of whole human blood stimulated with PMA, fMLP, OpZ, and A23187 was inhibited in a concentration-dependent way, indicating suppression of both receptor and nonreceptor activated chemiluminescence by resveratrol. Results from isolated human neutrophils revealed that resveratrol was active extracellularly as well as intracellularly in inhibiting the generation of reactive oxygen species. Liberation of ATP and analysis of apoptosis showed that in the concentration of 100 μM, resveratrol did not change the viability and integrity of isolated neutrophils. Western blot analysis documented that resveratrol in concentrations of 10 and 100 μM significantly decreased PMA-induced phosphorylation of PKC α/βII. Dose-dependent inhibition of nitrite production and iNOS protein expression in RAW 264.7 cells indicated possible interference of resveratrol with reactive nitrogen radical generation in professional phagocytes. The results suggest that resveratrol represents an effective naturally occurring substance with potent pharmacological effect on oxidative burst of human neutrophils and nitric oxide production by macrophages. It should be further investigated for its pharmacological activity against oxidative stress in ischaemia reperfusion, inflammation, and other pathological conditions, particularly neoplasia.