Uric acid modulates vascular endothelial function through the down regulation of nitric oxide ...
PAPEŽÍKOVÁ, Ivana, Michaela PEKAROVÁ, Hana KOLÁŘOVÁ, A KLINKE, D LAU, S BALDUS, Antonín LOJEK a Lukáš KUBALA. Uric acid modulates vascular endothelial function through the down regulation of nitric oxide production. Free Radical Res. Gordon and Breach, 2013. ISSN 1071-5762. Dostupné z: https://dx.doi.org/10.3109/10715762.2012.747677. |
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Základní údaje | |
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Originální název | Uric acid modulates vascular endothelial function through the down regulation of nitric oxide production. |
Název česky | Uric acid modulates vascular endothelial function through the down regulation of nitric oxide production. |
Název anglicky | Uric acid modulates vascular endothelial function through the down regulation of nitric oxide production. |
Autoři | PAPEŽÍKOVÁ, Ivana, Michaela PEKAROVÁ, Hana KOLÁŘOVÁ, A KLINKE, D LAU, S BALDUS, Antonín LOJEK a Lukáš KUBALA. |
Vydání | Free Radical Res. Gordon and Breach, 2013, 1071-5762. |
Další údaje | |
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Typ výsledku | Článek v odborném periodiku |
Utajení | není předmětem státního či obchodního tajemství |
WWW | URL |
Impakt faktor | Impact factor: 2.989 |
Doi | http://dx.doi.org/10.3109/10715762.2012.747677 |
Klíčová slova česky | hyperuricaemia, endothelial dysfunction, coronary artery disease, nitric oxide, endothelial nitric oxide synthase, reactive oxygen species, arginase activity |
Klíčová slova anglicky | hyperuricaemia, endothelial dysfunction, coronary artery disease, nitric oxide, endothelial nitric oxide synthase, reactive oxygen species, arginase activity |
Změnil | Změnila: Mgr. Michaela Pekarová, Ph.D., učo 85246. Změněno: 19. 1. 2017 14:09. |
Anotace |
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Endothelial dysfunction characterized by decreased nitric oxide (NO) bioavailability is the first stage of coronary artery disease. It is known that one of the factors associated with an increased risk of coronary artery disease is a high plasma level of uric acid. However, causative associations between hyperuricaemia and cardiovascular risk have not been definitely proved. In this work, we tested the effect of uric acid on endothelial NO bioavailability. Electrochemical measurement of NO production in acetylcholine-stimulated human umbilical endothelial cells (HUVECs) revealed that uric acid markedly decreases NO release. This finding was confirmed by organ bath experiments on mouse aortic segments. Uric acid dose-dependently reduced endothelium-dependent vasorelaxation. To reveal the mechanism of decreasing NO bioavailability we tested the effect of uric acid on reactive oxygen species production by HUVECs, on arginase activity, and on acetylcholine-induced endothelial NO synthase phosphorylation. It was found that uric acid increases arginase activity and reduces endothelial NO synthase phosphorylation. Interestingly, uric acid significantly increased intracellular superoxide formation. In conclusion, uric acid decreases NO bioavailability by means of multiple mechanisms. This finding supports the idea of a causal association between hyperuricaemia and cardiovascular risk. |
Anotace česky |
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Endothelial dysfunction characterized by decreased nitric oxide (NO) bioavailability is the first stage of coronary artery disease. It is known that one of the factors associated with an increased risk of coronary artery disease is a high plasma level of uric acid. However, causative associations between hyperuricaemia and cardiovascular risk have not been definitely proved. In this work, we tested the effect of uric acid on endothelial NO bioavailability. Electrochemical measurement of NO production in acetylcholine-stimulated human umbilical endothelial cells (HUVECs) revealed that uric acid markedly decreases NO release. This finding was confirmed by organ bath experiments on mouse aortic segments. Uric acid dose-dependently reduced endothelium-dependent vasorelaxation. To reveal the mechanism of decreasing NO bioavailability we tested the effect of uric acid on reactive oxygen species production by HUVECs, on arginase activity, and on acetylcholine-induced endothelial NO synthase phosphorylation. It was found that uric acid increases arginase activity and reduces endothelial NO synthase phosphorylation. Interestingly, uric acid significantly increased intracellular superoxide formation. In conclusion, uric acid decreases NO bioavailability by means of multiple mechanisms. This finding supports the idea of a causal association between hyperuricaemia and cardiovascular risk. |
Anotace anglicky |
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Endothelial dysfunction characterized by decreased nitric oxide (NO) bioavailability is the first stage of coronary artery disease. It is known that one of the factors associated with an increased risk of coronary artery disease is a high plasma level of uric acid. However, causative associations between hyperuricaemia and cardiovascular risk have not been definitely proved. In this work, we tested the effect of uric acid on endothelial NO bioavailability. Electrochemical measurement of NO production in acetylcholine-stimulated human umbilical endothelial cells (HUVECs) revealed that uric acid markedly decreases NO release. This finding was confirmed by organ bath experiments on mouse aortic segments. Uric acid dose-dependently reduced endothelium-dependent vasorelaxation. To reveal the mechanism of decreasing NO bioavailability we tested the effect of uric acid on reactive oxygen species production by HUVECs, on arginase activity, and on acetylcholine-induced endothelial NO synthase phosphorylation. It was found that uric acid increases arginase activity and reduces endothelial NO synthase phosphorylation. Interestingly, uric acid significantly increased intracellular superoxide formation. In conclusion, uric acid decreases NO bioavailability by means of multiple mechanisms. This finding supports the idea of a causal association between hyperuricaemia and cardiovascular risk. |
VytisknoutZobrazeno: 20. 5. 2024 20:22