Activity of aldehyde dehydrogenase in B-cell and plasma cell
subsets of monoclonal gammopathy patients and healthy donors

J 2017

Activity of aldehyde dehydrogenase in B-cell and plasma cell subsets of monoclonal gammopathy patients and healthy donors

VŠIANSKÁ, Pavla; Renata BEZDĚKOVÁ; Fedor KRYUKOV; Martina ALMÁŠI; Luděk POUR et. al.

Základní údaje

Originální název

Activity of aldehyde dehydrogenase in B-cell and plasma cell subsets of monoclonal gammopathy patients and healthy donors

Autoři

VŠIANSKÁ, Pavla; Renata BEZDĚKOVÁ; Fedor KRYUKOV; Martina ALMÁŠI; Luděk POUR; Miroslav PENKA; Roman HÁJEK a Lucie ŘÍHOVÁ

Vydání

European Journal of Haematology, Hoboken, Wiley-Blackwell, 2017, 0902-4441

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.595

Organizační jednotka

Lékařská fakulta

DOI

https://doi.org/10.1111/ejh.12779

UT WoS

000393166600002

Klíčová slova anglicky

aldehyde dehydrogenase; B-cell; multiple myeloma; plasma cell

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 3. 2018 16:30, Soňa Böhmová

Anotace

V originále

Background: Aldehyde dehydrogenase (ALDH) is highly active in physiological stem cells as well as in tumor-initiating cells of some malignancies including multiple myeloma (MM). Finding higher activity of ALDH in some cell subsets in monoclonal gammopathies (MG) could identify potential source of myeloma-initiating cells (MICs). Methods: Bone marrow of 12 MM, 9 monoclonal gammopathy of undetermined significance (MGUS), and 10 healthy donors (HD) were analyzed by flow cytometry. ALDH activity of B-cells and plasma cells (PC) was analyzed using Aldefluor. Results: Similar changes of ALDH activity were found during B-cell development in HD and MG. Decreasing of ALDH activity from immature to naive B-cells was found. In postgerminal stages, the activity started to increase, and in PCs, the ALDH activity was the same as in immature B-cells. Increased ALDH activity of all PC subsets compared to naive B-cells was found in MM as well as in HD, while in MGUS, only CD19-PCs have higher ALDH activity. In HD, ALDH activity was higher in CD19+PCs compared with MG. Conclusions: Our results indicate that changes of ALDH activity are the natural phenomenon in B-cell development; thus, high ALDH activity as a single marker is not appropriate for MICs identification.

Návaznosti

NV15-29508A, projekt VaV

Název: Cirkulující nukleové kyseliny jako markery progrese mnohočetného myelomu

Citovat

VŠIANSKÁ, Pavla; Renata BEZDĚKOVÁ; Fedor KRYUKOV; Martina ALMÁŠI; Luděk POUR; Miroslav PENKA; Roman HÁJEK a Lucie ŘÍHOVÁ. Activity of aldehyde dehydrogenase in B-cell and plasma cell subsets of monoclonal gammopathy patients and healthy donors. European Journal of Haematology. Hoboken: Wiley-Blackwell, 2017, roč. 98, č. 1, s. 19-25. ISSN 0902-4441. Dostupné z: https://doi.org/10.1111/ejh.12779.

@article{1371977,
   author = {Všianská, Pavla and Bezděková, Renata and Kryukov, Fedor and Almáši, Martina and Pour, Luděk and Penka, Miroslav and Hájek, Roman and Říhová, Lucie},
   article_location = {Hoboken},
   article_number = {1},
   doi = {https://doi.org/10.1111/ejh.12779},
   keywords = {aldehyde dehydrogenase; B-cell; multiple myeloma; plasma cell},
   language = {eng},
   issn = {0902-4441},
   journal = {European Journal of Haematology},
   title = {Activity of aldehyde dehydrogenase in B-cell and plasma cell subsets of monoclonal gammopathy patients and healthy donors},
   url = {http://dx.doi.org/10.1111/ejh.12779},
   volume = {98},
   year = {2017}
}

TY  - JOUR
ID  - 1371977
AU  - Všianská, Pavla - Bezděková, Renata - Kryukov, Fedor - Almáši, Martina - Pour, Luděk - Penka, Miroslav - Hájek, Roman - Říhová, Lucie
PY  - 2017
TI  - Activity of aldehyde dehydrogenase in B-cell and plasma cell subsets of monoclonal gammopathy patients and healthy donors
JF  - European Journal of Haematology
VL  - 98
IS  - 1
SP  - 19-25
EP  - 19-25
PB  - Wiley-Blackwell
SN  - 09024441
KW  - aldehyde dehydrogenase
KW  - B-cell
KW  - multiple myeloma
KW  - plasma cell
UR  - http://dx.doi.org/10.1111/ejh.12779
L2  - http://dx.doi.org/10.1111/ejh.12779
N2  - Background: Aldehyde dehydrogenase (ALDH) is highly active in physiological stem cells as well as in tumor-initiating cells of some malignancies including multiple myeloma (MM). Finding higher activity of ALDH in some cell subsets in monoclonal gammopathies (MG) could identify potential source of myeloma-initiating cells (MICs). Methods: Bone marrow of 12 MM, 9 monoclonal gammopathy of undetermined significance (MGUS), and 10 healthy donors (HD) were analyzed by flow cytometry. ALDH activity of B-cells and plasma cells (PC) was analyzed using Aldefluor. Results: Similar changes of ALDH activity were found during B-cell development in HD and MG. Decreasing of ALDH activity from immature to naive B-cells was found. In postgerminal stages, the activity started to increase, and in PCs, the ALDH activity was the same as in immature B-cells. Increased ALDH activity of all PC subsets compared to naive B-cells was found in MM as well as in HD, while in MGUS, only CD19-PCs have higher ALDH activity. In HD, ALDH activity was higher in CD19+PCs compared with MG. Conclusions: Our results indicate that changes of ALDH activity are the natural phenomenon in B-cell development; thus, high ALDH activity as a single marker is not appropriate for MICs identification.
ER  -

VŠIANSKÁ, Pavla; Renata BEZDĚKOVÁ; Fedor KRYUKOV; Martina ALMÁŠI; Luděk POUR; Miroslav PENKA; Roman HÁJEK a Lucie ŘÍHOVÁ. Activity of aldehyde dehydrogenase in B-cell and plasma cell subsets of monoclonal gammopathy patients and healthy donors. \textit{European Journal of Haematology}. Hoboken: Wiley-Blackwell, 2017, roč.~98, č.~1, s.~19-25. ISSN~0902-4441. Dostupné z: https://doi.org/10.1111/ejh.12779.

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