Metabolism of drugs and other xenobiotics in giant liver fluke
(Fascioloides magna)

J 2016

Metabolism of drugs and other xenobiotics in giant liver fluke (Fascioloides magna)

PRCHAL, Lukáš, Ivan VOKŘÁL, Martin KAŠNÝ, Lenka REJŠKOVÁ, Markéta ZAJÍČKOVÁ et. al.

Základní údaje

Originální název

Metabolism of drugs and other xenobiotics in giant liver fluke (Fascioloides magna)

Autoři

PRCHAL, Lukáš (203 Česká republika), Ivan VOKŘÁL (203 Česká republika), Martin KAŠNÝ (203 Česká republika, garant, domácí), Lenka REJŠKOVÁ (203 Česká republika), Markéta ZAJÍČKOVÁ (203 Česká republika), Jiří LAMKA (203 Česká republika), Lenka SKÁLOVÁ (203 Česká republika), Lenka LECOVÁ (203 Česká republika) a Barbora SZOTÁKOVÁ (203 Česká republika)

Vydání

Xenobiotica, UK, Taylor & Francis Group, 2016, 0049-8254

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 1.932

Kód RIV

RIV/00216224:14310/16:00093531

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.3109/00498254.2015.1060370

UT WoS

000369275500005

Klíčová slova anglicky

Albendazole; anthelmintics; biotransformation; drug-metabolizing enzymes; mebendazole

Štítky

AKR, rivok

Změněno: 7. 3. 2018 10:43, Mgr. Lucie Jarošová, DiS.

Anotace

V originále

1.Giant liver fluke Fascioloides magna is a dangerous parasite, which infects herbivores. It was imported to Europe from North America and started to spread. Benzimidazoles like albendazole, mebendazole, triclabendazole and salicylanilides closantel and rafoxanide are the most used anthelmintics to control fascioloidosis. However their effect might be altered via drug-metabolizing enzymes of this parasite.2.The aim of our study was to determine the activities of drug-metabolizing enzymes in F. magna and the metabolism of above mentioned anthelmintics.3.Activities of several oxidative, reductive and conjugative enzymes towards various model xenobiotic substrates were found in F. magna subcellular fractions.4.Subcellular fractions from F. magna oxidized albendazole to its sulphoxide metabolite and reduced mebendazole to hydroxyl-mebendazole. Under ex vivo conditions, only very-low concentrations of these compounds were detected using high-performance liquid chromatography/mass spectrometry.5.The results indicate that the giant liver fluke possesses the active xenobiotic-metabolizing system. The overexpression of this system may play an important role in parasite resistance against these anthelmintics.

Návaznosti

MUNI/A/1325/2015, interní kód MU

Název: Analýzy diverzity biologických systémů různých úrovní a na různých škálách prostředí (Akronym: BIDA5)

Investor: Masarykova univerzita, Analýzy diverzity biologických systémů různých úrovní a na různých škálách prostředí, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

PRCHAL, Lukáš, Ivan VOKŘÁL, Martin KAŠNÝ, Lenka REJŠKOVÁ, Markéta ZAJÍČKOVÁ, Jiří LAMKA, Lenka SKÁLOVÁ, Lenka LECOVÁ a Barbora SZOTÁKOVÁ. Metabolism of drugs and other xenobiotics in giant liver fluke (Fascioloides magna). Xenobiotica. UK: Taylor & Francis Group, 2016, roč. 46, č. 2, s. 132-140. ISSN 0049-8254. Dostupné z: https://dx.doi.org/10.3109/00498254.2015.1060370.

@article{1372489,
   author = {Prchal, Lukáš and Vokřál, Ivan and Kašný, Martin and Rejšková, Lenka and Zajíčková, Markéta and Lamka, Jiří and Skálová, Lenka and Lecová, Lenka and Szotáková, Barbora},
   article_location = {UK},
   article_number = {2},
   doi = {http://dx.doi.org/10.3109/00498254.2015.1060370},
   keywords = {Albendazole; anthelmintics; biotransformation; drug-metabolizing enzymes; mebendazole},
   language = {eng},
   issn = {0049-8254},
   journal = {Xenobiotica},
   title = {Metabolism of drugs and other xenobiotics in giant liver fluke (Fascioloides magna)},
   volume = {46},
   year = {2016}
}

TY  - JOUR
ID  - 1372489
AU  - Prchal, Lukáš - Vokřál, Ivan - Kašný, Martin - Rejšková, Lenka - Zajíčková, Markéta - Lamka, Jiří - Skálová, Lenka - Lecová, Lenka - Szotáková, Barbora
PY  - 2016
TI  - Metabolism of drugs and other xenobiotics in giant liver fluke (Fascioloides magna)
JF  - Xenobiotica
VL  - 46
IS  - 2
SP  - 132-140
EP  - 132-140
PB  - Taylor & Francis Group
SN  - 00498254
KW  - Albendazole
KW  - anthelmintics
KW  - biotransformation
KW  - drug-metabolizing enzymes
KW  - mebendazole
N2  - 1.Giant liver fluke Fascioloides magna is a dangerous parasite, which infects herbivores. It was imported to Europe from North America and started to spread. Benzimidazoles like albendazole, mebendazole, triclabendazole and salicylanilides closantel and rafoxanide are the most used anthelmintics to control fascioloidosis. However their effect might be altered via drug-metabolizing enzymes of this parasite.2.The aim of our study was to determine the activities of drug-metabolizing enzymes in F. magna and the metabolism of above mentioned anthelmintics.3.Activities of several oxidative, reductive and conjugative enzymes towards various model xenobiotic substrates were found in F. magna subcellular fractions.4.Subcellular fractions from F. magna oxidized albendazole to its sulphoxide metabolite and reduced mebendazole to hydroxyl-mebendazole. Under ex vivo conditions, only very-low concentrations of these compounds were detected using high-performance liquid chromatography/mass spectrometry.5.The results indicate that the giant liver fluke possesses the active xenobiotic-metabolizing system. The overexpression of this system may play an important role in parasite resistance against these anthelmintics.
ER  -

PRCHAL, Lukáš, Ivan VOKŘÁL, Martin KAŠNÝ, Lenka REJŠKOVÁ, Markéta ZAJÍČKOVÁ, Jiří LAMKA, Lenka SKÁLOVÁ, Lenka LECOVÁ a Barbora SZOTÁKOVÁ. Metabolism of drugs and other xenobiotics in giant liver fluke (Fascioloides magna). \textit{Xenobiotica}. UK: Taylor \&{} Francis Group, 2016, roč.~46, č.~2, s.~132-140. ISSN~0049-8254. Dostupné z: https://dx.doi.org/10.3109/00498254.2015.1060370.

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