Scrape Loading/Dye Transfer Assay

C 2016

Scrape Loading/Dye Transfer Assay

BABICA, Pavel; Iva SOVADINOVÁ a Brad L. UPHAM

Základní údaje

Originální název

Scrape Loading/Dye Transfer Assay

Autoři

BABICA, Pavel; Iva SOVADINOVÁ a Brad L. UPHAM

Vydání

New York, USA, Gap Junction Protocols, od s. 133-144, 12 s. Methods in Molecular Biology, 2016

Nakladatel

Springer New York

Další údaje

Jazyk

angličtina

Typ výsledku

Kapitola resp. kapitoly v odborné knize

Obor

10608 Biochemistry and molecular biology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

elektronická verze "online"

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/16:00093583

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-1-4939-3664-9

DOI

https://doi.org/10.1007/978-1-4939-3664-9

UT WoS

000381766400010

Klíčová slova anglicky

Dye coupling; Dye transfer; Ex vivo assessment; Gap junctional intercellular communication assessment; High throughput; In vitro assay; Incision loading; Lucifer Yellow; Scalpel loading; Scrape loading; Tracers

Štítky

AKR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 23. 4. 2020 12:49, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

The scrape loading/dye transfer (SL/DT) technique is a simple functional assay for the simultaneous assessment of gap junctional intercellular communication (GJIC) in a large population of cells. The equipment needs are minimal and are typically met in standard cell biology labs, and SL/DT is the simplest and quickest of all the assays that measure GJIC. This assay has also been adapted for in vivo studies. The SL/DT assay is also conducive to a high-throughput setup with automated fluorescence microscopy imaging and analysis to elucidate more samples in shorter time, and hence can serve a broad range of in vitro pharmacological and toxicological needs.

Návaznosti

LM2015051, projekt VaV

Název: Centrum pro výzkum toxických látek v prostředí (Akronym: RECETOX RI)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Výzkumná infrastruktura RECETOX

LO1214, projekt VaV

Název: Centrum pro výzkum toxických látek v prostředí (Akronym: RECETOX)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Centrum pro výzkum toxických látek v prostředí

Citovat

BABICA, Pavel; Iva SOVADINOVÁ a Brad L. UPHAM. Scrape Loading/Dye Transfer Assay. Online. In Vinken, Mathieu; Johnstone, Scott R. Gap Junction Protocols. New York, USA: Springer New York, 2016, s. 133-144. Methods in Molecular Biology. ISBN 978-1-4939-3664-9. Dostupné z: https://doi.org/10.1007/978-1-4939-3664-9.

@inbook{1372749,
   author = {Babica, Pavel and Sovadinová, Iva and Upham, Brad L.},
   address = {New York, USA},
   booktitle = {Gap Junction Protocols},
   doi = {https://doi.org/10.1007/978-1-4939-3664-9},
   editor = {Vinken, Mathieu; Johnstone, Scott R.},
   keywords = {Dye coupling; Dye transfer; Ex vivo assessment; Gap junctional intercellular communication assessment; High throughput; In vitro assay; Incision loading; Lucifer Yellow; Scalpel loading; Scrape loading; Tracers},
   howpublished = {elektronická verze "online"},
   language = {eng},
   location = {New York, USA},
   isbn = {978-1-4939-3664-9},
   pages = {133-144},
   publisher = {Springer New York},
   title = {Scrape Loading/Dye Transfer Assay},
   url = {http://link.springer.com/protocol/10.1007%2F978-1-4939-3664-9_9},
   year = {2016}
}

TY  - CHAP
ID  - 1372749
AU  - Babica, Pavel - Sovadinová, Iva - Upham, Brad L.
PY  - 2016
TI  - Scrape Loading/Dye Transfer Assay
VL  - Methods in Molecular Biology
PB  - Springer New York
CY  - New York, USA
SN  - 9781493936649
KW  - Dye coupling
KW  - Dye transfer
KW  - Ex vivo assessment
KW  - Gap junctional intercellular communication assessment
KW  - High throughput
KW  - In vitro assay
KW  - Incision loading
KW  - Lucifer Yellow
KW  - Scalpel loading
KW  - Scrape loading
KW  - Tracers
UR  - http://link.springer.com/protocol/10.1007%2F978-1-4939-3664-9_9
L2  - http://link.springer.com/protocol/10.1007%2F978-1-4939-3664-9_9
N2  - The scrape loading/dye transfer (SL/DT) technique is a simple functional assay for the simultaneous assessment of gap junctional intercellular communication (GJIC) in a large population of cells. The equipment needs are minimal and are typically met in standard cell biology labs, and SL/DT is the simplest and quickest of all the assays that measure GJIC. This assay has also been adapted for in vivo studies. The SL/DT assay is also conducive to a high-throughput setup with automated fluorescence microscopy imaging and analysis to elucidate more samples in shorter time, and hence can serve a broad range of in vitro pharmacological and toxicological needs.
ER  -

BABICA, Pavel; Iva SOVADINOVÁ a Brad L. UPHAM. Scrape Loading/Dye Transfer Assay. Online. In Vinken, Mathieu; Johnstone, Scott R. \textit{Gap Junction Protocols}. New York, USA: Springer New York, 2016, s.~133-144. Methods in Molecular Biology. ISBN~978-1-4939-3664-9. Dostupné z: https://doi.org/10.1007/978-1-4939-3664-9.

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