Phenotypic reversion in fas mutants of Arabidopsis thaliana by
reintroduction of FAS genes: variable recovery of telomeres
with major spatial rearrangements and transcriptional
reprogramming of 45S rDNA genes

J 2016

Phenotypic reversion in fas mutants of Arabidopsis thaliana by reintroduction of FAS genes: variable recovery of telomeres with major spatial rearrangements and transcriptional reprogramming of 45S rDNA genes

PAVLIŠTOVÁ, Veronika; Martina DVOŘÁČKOVÁ; Michal JEŽ; Iva MOZGOVÁ; Petr MOKROŠ et al.

Základní údaje

Originální název

Phenotypic reversion in fas mutants of Arabidopsis thaliana by reintroduction of FAS genes: variable recovery of telomeres with major spatial rearrangements and transcriptional reprogramming of 45S rDNA genes

Autoři

PAVLIŠTOVÁ, Veronika; Martina DVOŘÁČKOVÁ ; Michal JEŽ; Iva MOZGOVÁ; Petr MOKROŠ a Jiří FAJKUS

Vydání

Plant Journal, Hoboken, Wiley-Blackwell, 2016, 0960-7412

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 5.901

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/16:00088685

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

CHROMATIN ASSEMBLY FACTOR-1; RIBOSOMAL-RNA GENES; DOSAGE CONTROL; FACTOR CAF-1; REPEATS; TRANSFORMATION; RECOMBINATION; MAINTENANCE; DYSFUNCTION; SITES

Štítky

rivok

Změněno: 14. 3. 2017 13:39, Mgr. Eva Špillingová

Anotace

V originále

Arabidopsis thaliana mutants dysfunctional in the evolutionarily conserved protein complex chromatin assembly factor-1 (CAF-1), which deposits the canonical histone H3 variant H3.1 during DNA synthesis-dependent chromatin assembly, display complex phenotypic changes including meristem and growth alterations, sensitivity to DNA-damaging agents, and reduced fertility. We reported previously that mutants in the FAS1 subunit of CAF-1 progressively lose telomere and 45S rDNA repeats. Here we show that multiple aspects of the fas phenotype are recovered immediately on expression of a reintroduced FAS1 allele, and are clearly independent of the recovery of rDNA copy-numbers and telomeres. In reverted lines, 45S rDNA genes are recovered to diverse levels with a strikingly different representation of their variants, and the typical association of nucleolar organizing region 4 with the nucleolus is perturbed. One of 45S rDNA variants (VAR1), which is silenced in wild-type (WT) plants without mutation history (Col-0 WT), dominates the expression pattern, whereas VAR2 is dominant in Col-0 WT plants. We propose an explanation for the variability of telomere and 45S rDNA repeats associated with CAF-1 function, suggesting that the differences in nuclear partitioning and expression of the rDNA variants in fas mutants and their revertants provide a useful experimental system to study genetic and epigenetic factors in gene dosage compensation.

Návaznosti

GAP501/11/0289, projekt VaV

Název: Ztráta specifických repetic DNA v důsledku dysfunkce CAF1 u rostlin

Investor: Grantová agentura ČR, Ztráta specifických repetic DNA v důsledku dysfunkce CAF1 u rostlin

GA16-01137S, projekt VaV

Název: Faktory genomové stability u mechu a vyšších rostlin

Investor: Grantová agentura ČR, Faktory genomové stability u mechu a vyšších rostlin

GJ16-04166Y, projekt VaV

Název: Chromatin asociované faktory podílející se na regulaci 45SrDNA v Arabidopsis

Investor: Grantová agentura ČR, Chromatin asociované faktory podílející se na regulaci 45SrDNA v Arabidopsis

LH15189, projekt VaV

Název: Struktura a dynamika 45S rDNA lokusů v Arabidopsis thaliana

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, Struktura a dynamika 45S rDNA lokusů v Arabidopsis thaliana

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

PAVLIŠTOVÁ, Veronika; Martina DVOŘÁČKOVÁ; Michal JEŽ; Iva MOZGOVÁ; Petr MOKROŠ a Jiří FAJKUS. Phenotypic reversion in fas mutants of Arabidopsis thaliana by reintroduction of FAS genes: variable recovery of telomeres with major spatial rearrangements and transcriptional reprogramming of 45S rDNA genes. Plant Journal. Hoboken: Wiley-Blackwell, 2016, roč. 88, č. 3, s. 411-424. ISSN 0960-7412. Dostupné z: https://doi.org/10.1111/tpj.13257.

@article{1373251,
   author = {Pavlištová, Veronika and Dvořáčková, Martina and Jež, Michal and Mozgová, Iva and Mokroš, Petr and Fajkus, Jiří},
   article_location = {Hoboken},
   article_number = {3},
   doi = {https://doi.org/10.1111/tpj.13257},
   keywords = {CHROMATIN ASSEMBLY FACTOR-1; RIBOSOMAL-RNA GENES; DOSAGE CONTROL; FACTOR CAF-1; REPEATS; TRANSFORMATION; RECOMBINATION; MAINTENANCE; DYSFUNCTION; SITES},
   language = {eng},
   issn = {0960-7412},
   journal = {Plant Journal},
   title = {Phenotypic reversion in fas mutants of Arabidopsis thaliana by reintroduction of FAS genes: variable recovery of telomeres with major spatial rearrangements and transcriptional reprogramming of 45S rDNA genes},
   url = {http://onlinelibrary.wiley.com/doi/10.1111/tpj.13257/abstract},
   volume = {88},
   year = {2016}
}

TY  - JOUR
ID  - 1373251
AU  - Pavlištová, Veronika - Dvořáčková, Martina - Jež, Michal - Mozgová, Iva - Mokroš, Petr - Fajkus, Jiří
PY  - 2016
TI  - Phenotypic reversion in fas mutants of Arabidopsis thaliana by reintroduction of FAS genes: variable recovery of telomeres with major spatial rearrangements and transcriptional reprogramming of 45S rDNA genes
JF  - Plant Journal
VL  - 88
IS  - 3
SP  - 411-424
EP  - 411-424
PB  - Wiley-Blackwell
SN  - 09607412
KW  - CHROMATIN ASSEMBLY FACTOR-1
KW  - RIBOSOMAL-RNA GENES
KW  - DOSAGE CONTROL
KW  - FACTOR CAF-1
KW  - REPEATS
KW  - TRANSFORMATION
KW  - RECOMBINATION
KW  - MAINTENANCE
KW  - DYSFUNCTION
KW  - SITES
UR  - http://onlinelibrary.wiley.com/doi/10.1111/tpj.13257/abstract
L2  - http://onlinelibrary.wiley.com/doi/10.1111/tpj.13257/abstract
N2  - Arabidopsis thaliana mutants dysfunctional in the evolutionarily conserved protein complex chromatin assembly factor-1 (CAF-1), which deposits the canonical histone H3 variant H3.1 during DNA synthesis-dependent chromatin assembly, display complex phenotypic changes including meristem and growth alterations, sensitivity to DNA-damaging agents, and reduced fertility. We reported previously that mutants in the FAS1 subunit of CAF-1 progressively lose telomere and 45S rDNA repeats. Here we show that multiple aspects of the fas phenotype are recovered immediately on expression of a reintroduced FAS1 allele, and are clearly independent of the recovery of rDNA copy-numbers and telomeres. In reverted lines, 45S rDNA genes are recovered to diverse levels with a strikingly different representation of their variants, and the typical association of nucleolar organizing region 4 with the nucleolus is perturbed. One of 45S rDNA variants (VAR1), which is silenced in wild-type (WT) plants without mutation history (Col-0 WT), dominates the expression pattern, whereas VAR2 is dominant in Col-0 WT plants. We propose an explanation for the variability of telomere and 45S rDNA repeats associated with CAF-1 function, suggesting that the differences in nuclear partitioning and expression of the rDNA variants in fas mutants and their revertants provide a useful experimental system to study genetic and epigenetic factors in gene dosage compensation.
ER  -

PAVLIŠTOVÁ, Veronika; Martina DVOŘÁČKOVÁ; Michal JEŽ; Iva MOZGOVÁ; Petr MOKROŠ a Jiří FAJKUS. Phenotypic reversion in fas mutants of Arabidopsis thaliana by reintroduction of FAS genes: variable recovery of telomeres with major spatial rearrangements and transcriptional reprogramming of 45S rDNA genes. \textit{Plant Journal}. Hoboken: Wiley-Blackwell, 2016, roč.~88, č.~3, s.~411-424. ISSN~0960-7412. Dostupné z: https://doi.org/10.1111/tpj.13257.

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