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@article{1373251, author = {Pavlištová, Veronika and Dvořáčková, Martina and Jež, Michal and Mozgová, Iva and Mokroš, Petr and Fajkus, Jiří}, article_location = {Hoboken}, article_number = {3}, doi = {http://dx.doi.org/10.1111/tpj.13257}, keywords = {CHROMATIN ASSEMBLY FACTOR-1; RIBOSOMAL-RNA GENES; DOSAGE CONTROL; FACTOR CAF-1; REPEATS; TRANSFORMATION; RECOMBINATION; MAINTENANCE; DYSFUNCTION; SITES}, language = {eng}, issn = {0960-7412}, journal = {Plant Journal}, title = {Phenotypic reversion in fas mutants of Arabidopsis thaliana by reintroduction of FAS genes: variable recovery of telomeres with major spatial rearrangements and transcriptional reprogramming of 45S rDNA genes}, url = {http://onlinelibrary.wiley.com/doi/10.1111/tpj.13257/abstract}, volume = {88}, year = {2016} }
TY - JOUR ID - 1373251 AU - Pavlištová, Veronika - Dvořáčková, Martina - Jež, Michal - Mozgová, Iva - Mokroš, Petr - Fajkus, Jiří PY - 2016 TI - Phenotypic reversion in fas mutants of Arabidopsis thaliana by reintroduction of FAS genes: variable recovery of telomeres with major spatial rearrangements and transcriptional reprogramming of 45S rDNA genes JF - Plant Journal VL - 88 IS - 3 SP - 411-424 EP - 411-424 PB - Wiley-Blackwell SN - 09607412 KW - CHROMATIN ASSEMBLY FACTOR-1 KW - RIBOSOMAL-RNA GENES KW - DOSAGE CONTROL KW - FACTOR CAF-1 KW - REPEATS KW - TRANSFORMATION KW - RECOMBINATION KW - MAINTENANCE KW - DYSFUNCTION KW - SITES UR - http://onlinelibrary.wiley.com/doi/10.1111/tpj.13257/abstract L2 - http://onlinelibrary.wiley.com/doi/10.1111/tpj.13257/abstract N2 - Arabidopsis thaliana mutants dysfunctional in the evolutionarily conserved protein complex chromatin assembly factor-1 (CAF-1), which deposits the canonical histone H3 variant H3.1 during DNA synthesis-dependent chromatin assembly, display complex phenotypic changes including meristem and growth alterations, sensitivity to DNA-damaging agents, and reduced fertility. We reported previously that mutants in the FAS1 subunit of CAF-1 progressively lose telomere and 45S rDNA repeats. Here we show that multiple aspects of the fas phenotype are recovered immediately on expression of a reintroduced FAS1 allele, and are clearly independent of the recovery of rDNA copy-numbers and telomeres. In reverted lines, 45S rDNA genes are recovered to diverse levels with a strikingly different representation of their variants, and the typical association of nucleolar organizing region 4 with the nucleolus is perturbed. One of 45S rDNA variants (VAR1), which is silenced in wild-type (WT) plants without mutation history (Col-0 WT), dominates the expression pattern, whereas VAR2 is dominant in Col-0 WT plants. We propose an explanation for the variability of telomere and 45S rDNA repeats associated with CAF-1 function, suggesting that the differences in nuclear partitioning and expression of the rDNA variants in fas mutants and their revertants provide a useful experimental system to study genetic and epigenetic factors in gene dosage compensation. ER -
PAVLIŠTOVÁ, Veronika, Martina DVOŘÁČKOVÁ, Michal JEŽ, Iva MOZGOVÁ, Petr MOKROŠ a Jiří FAJKUS. Phenotypic reversion in fas mutants of Arabidopsis thaliana by reintroduction of FAS genes: variable recovery of telomeres with major spatial rearrangements and transcriptional reprogramming of 45S rDNA genes. \textit{Plant Journal}. Hoboken: Wiley-Blackwell, 2016, roč.~88, č.~3, s.~411-424. ISSN~0960-7412. Dostupné z: https://dx.doi.org/10.1111/tpj.13257.
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