High intraspecies heterogeneity within Staphylococcus sciuri
and rejection of its classification into S. sciuri subsp.
sciuri, S. sciuri subsp. carnaticus and S. sciuri subsp.
rodentium

J 2016

High intraspecies heterogeneity within Staphylococcus sciuri and rejection of its classification into S. sciuri subsp. sciuri, S. sciuri subsp. carnaticus and S. sciuri subsp. rodentium

ŠVEC, Pavel, Petr PETRÁŠ, Roman PANTŮČEK, Jiří DOŠKAŘ, Ivo SEDLÁČEK et. al.

Základní údaje

Originální název

High intraspecies heterogeneity within Staphylococcus sciuri and rejection of its classification into S. sciuri subsp. sciuri, S. sciuri subsp. carnaticus and S. sciuri subsp. rodentium

Autoři

ŠVEC, Pavel (203 Česká republika, garant, domácí), Petr PETRÁŠ (203 Česká republika), Roman PANTŮČEK (203 Česká republika, domácí), Jiří DOŠKAŘ (203 Česká republika, domácí) a Ivo SEDLÁČEK (203 Česká republika, domácí)

Vydání

International Journal of Systematic and Evolutionary Microbiology, Society for General Microbiology, Great Britain, 2016, 1466-5026

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.134

Kód RIV

RIV/00216224:14310/16:00093669

Organizační jednotka

Přírodovědecká fakulta

DOI

http://dx.doi.org/10.1099/ijsem.0.001493

UT WoS

000393357900046

Klíčová slova anglicky

Staphylococcus sciuri; subspecies; reclassification; taxonomy

Štítky

AKR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 26. 10. 2020 10:43, prof. RNDr. Roman Pantůček, Ph.D.

Anotace

V originále

A polyphasic taxonomic approach was applied to strains of the species Staphylococcus sciuri in order to clarify the taxonomic legitimacy of the delineation of S. sciuri into S. sciuri subsp. sciuri , S. sciuri subsp. carnaticus and S. sciuri subsp. rodentium. A group of 81 S. sciuri isolates obtained from human (n=62) and veterinary (n=17) clinical materials and foods (n=2) and ten reference and type strains obtained from the Czech Collection of Microorganisms were characterized by extensive biotyping using conventional tests and commercial identification kits (ID 32 Staph, STAPHYtest, Biolog Microbial ID System), matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry, automated ribotyping with EcoRI restriction enzyme, 16S–23S rRNA gene intergenic transcribed spacer PCR fingerprinting and repetitive sequence-based PCR fingerprinting with the (GTG)5 primer. Selected strains representing different ribotypes were further characterized using sequencing of the beta-subunit of RNA polymerase (rpoB) gene. Individual techniques revealed high heterogeneity within the analysed S. sciuri strains but differentiation of the investigated strains into groups corresponding to the aforementioned S. sciuri subspecies and supported by these techniques was not clearly revealed. Based on obtained results and data retrieved from literature we propose rejecting the separation of S. sciuri species into S. sciuri subsp. sciuri , S. sciuri subsp. carnaticus and S. sciuri subsp. rodentium and we suggest reclassification these subspecies as S. sciuri with the type strain W.E. Kloos SC 116T (=ATCC 29062T=BCRC 12927T=CCM 3473T=CCUG 15598T=CNCTC 5683T=DSM 20345T=JCM 2425T=NCTC 12103T).

Návaznosti

MUNI/A/0967/2015, interní kód MU

Název: Podpora výzkumné činnosti studentů molekulární biologie a genetiky 4 (Akronym: MBG4)

Investor: Masarykova univerzita, Podpora výzkumné činnosti studentů molekulární biologie a genetiky 4, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

ŠVEC, Pavel, Petr PETRÁŠ, Roman PANTŮČEK, Jiří DOŠKAŘ a Ivo SEDLÁČEK. High intraspecies heterogeneity within Staphylococcus sciuri and rejection of its classification into S. sciuri subsp. sciuri, S. sciuri subsp. carnaticus and S. sciuri subsp. rodentium. International Journal of Systematic and Evolutionary Microbiology. Society for General Microbiology, Great Britain, 2016, roč. 66, December, s. 5181-5186. ISSN 1466-5026. Dostupné z: https://dx.doi.org/10.1099/ijsem.0.001493.

@article{1373455,
   author = {Švec, Pavel and Petráš, Petr and Pantůček, Roman and Doškař, Jiří and Sedláček, Ivo},
   article_number = {December},
   doi = {http://dx.doi.org/10.1099/ijsem.0.001493},
   keywords = {Staphylococcus sciuri; subspecies; reclassification; taxonomy},
   language = {eng},
   issn = {1466-5026},
   journal = {International Journal of Systematic and Evolutionary Microbiology},
   title = {High intraspecies heterogeneity within Staphylococcus sciuri and rejection of its classification into S. sciuri subsp. sciuri, S. sciuri subsp. carnaticus and S. sciuri subsp. rodentium},
   url = {https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001493},
   volume = {66},
   year = {2016}
}

TY  - JOUR
ID  - 1373455
AU  - Švec, Pavel - Petráš, Petr - Pantůček, Roman - Doškař, Jiří - Sedláček, Ivo
PY  - 2016
TI  - High intraspecies heterogeneity within Staphylococcus sciuri and rejection of its classification into S. sciuri subsp. sciuri, S. sciuri subsp. carnaticus and S. sciuri subsp. rodentium
JF  - International Journal of Systematic and Evolutionary Microbiology
VL  - 66
IS  - December
SP  - 5181-5186
EP  - 5181-5186
PB  - Society for General Microbiology, Great Britain
SN  - 14665026
KW  - Staphylococcus sciuri
KW  - subspecies
KW  - reclassification
KW  - taxonomy
UR  - https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001493
L2  - https://www.microbiologyresearch.org/content/journal/ijsem/10.1099/ijsem.0.001493
N2  - A polyphasic taxonomic approach was applied to strains of the species Staphylococcus sciuri in order to clarify the taxonomic legitimacy of the delineation of S. sciuri into S. sciuri subsp. sciuri , S. sciuri subsp. carnaticus and S. sciuri subsp. rodentium. A group of 81 S. sciuri isolates obtained from human (n=62) and veterinary (n=17) clinical materials and foods (n=2) and ten reference and type strains obtained from the Czech Collection of Microorganisms were characterized by extensive biotyping using conventional tests and commercial identification kits (ID 32 Staph, STAPHYtest, Biolog Microbial ID System), matrix-assisted laser-desorption/ionization time-of-flight mass spectrometry, automated ribotyping with EcoRI restriction enzyme, 16S–23S rRNA gene intergenic transcribed spacer PCR fingerprinting and repetitive sequence-based PCR fingerprinting with the (GTG)5 primer. Selected strains representing different ribotypes were further characterized using sequencing of the beta-subunit of RNA polymerase (rpoB) gene. Individual techniques revealed high heterogeneity within the analysed S. sciuri strains but differentiation of the investigated strains into groups corresponding to the aforementioned S. sciuri subspecies and supported by these techniques was not clearly revealed. Based on obtained results and data retrieved from literature we propose rejecting the separation of S. sciuri species into S. sciuri subsp. sciuri , S. sciuri subsp. carnaticus and S. sciuri subsp. rodentium and we suggest reclassification these subspecies as S. sciuri with the type strain W.E. Kloos SC 116T (=ATCC 29062T=BCRC 12927T=CCM 3473T=CCUG 15598T=CNCTC 5683T=DSM 20345T=JCM 2425T=NCTC 12103T).
ER  -

ŠVEC, Pavel, Petr PETRÁŠ, Roman PANTŮČEK, Jiří DOŠKAŘ a Ivo SEDLÁČEK. High intraspecies heterogeneity within Staphylococcus sciuri and rejection of its classification into S. sciuri subsp. sciuri, S. sciuri subsp. carnaticus and S. sciuri subsp. rodentium. \textit{International Journal of Systematic and Evolutionary Microbiology}. Society for General Microbiology, Great Britain, 2016, roč.~66, December, s.~5181-5186. ISSN~1466-5026. Dostupné z: https://dx.doi.org/10.1099/ijsem.0.001493.

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