De-novo development of microsatellite markers for Dactylogyrus
vistulae

a 2016

De-novo development of microsatellite markers for Dactylogyrus vistulae

GETTOVÁ, Lenka a Andrea VETEŠNÍKOVÁ ŠIMKOVÁ

Základní údaje

Originální název

De-novo development of microsatellite markers for Dactylogyrus vistulae

Autoři

GETTOVÁ, Lenka (703 Slovensko, domácí) a Andrea VETEŠNÍKOVÁ ŠIMKOVÁ (203 Česká republika, garant, domácí)

Vydání

5th Workshop of the European Centre of IchtyoParasitology, 2016

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/16:00088713

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-210-8373-8

Klíčová slova anglicky

Monogenea; Dactylogyrus; microsatellites

Změněno: 27. 2. 2017 12:04, Mgr. Lenka Gettová

Anotace

V originále

In our study, we aimed to characterize de-novo microsatellite markers for Dactylogyrus parasites. Dactylogyrus vistulae was selected as a model organism owing to its relatively large body size in comparison to the other members of this genus. Approximately 300 specimens of D. vistulae were collected from European chub Squalius cephalus of the Dyje River. Genomic DNA was isolated and subsequently prepared genomic libraries were subjected to Illumina MiSeq (provided by CEITEC, MU, Brno). QDD program (Meglécz et al. 2010) was used for selecting microsatellite markers and primer design. Following criteria used in Gettová et al. (2013) 70 sequences with target microsatellites were selected. Monogenean parasites, especially dactylogyrids, display a high level of host specificity. As a result genetic structure of monogenean parasites might be host-dependent. Microsatellite markers developed for Dactylogyrus parasites might, therefore, enable us to reconstruct the evolution history of their fish hosts. In addition, in the case of rare generalist Dactylogyrus parasites (such is D. vistulae), we expect that the microsatellites will represent a useful tool to analyse the structure of parasite populations parasitizing different host species and/or geographically isolated parasite populations across Europe.

Návaznosti

GAP505/12/0375, projekt VaV

Název: Evolučně-imunitní a ekologické aspekty parazitace u hybridních a polyploidních kaprovitých ryb

Investor: Grantová agentura ČR, Evolučně-imunitní a ekologické aspekty parazitace u hybridních a polypoidních kaprovitých ryb

Citovat

GETTOVÁ, Lenka a Andrea VETEŠNÍKOVÁ ŠIMKOVÁ. De-novo development of microsatellite markers for Dactylogyrus vistulae. In 5th Workshop of the European Centre of IchtyoParasitology. 2016. ISBN 978-80-210-8373-8.

@proceedings{1373961,
   author = {Gettová, Lenka and Vetešníková Šimková, Andrea},
   booktitle = {5th Workshop of the European Centre of IchtyoParasitology},
   keywords = {Monogenea; Dactylogyrus; microsatellites},
   language = {eng},
   isbn = {978-80-210-8373-8},
   title = {De-novo development of microsatellite markers for Dactylogyrus vistulae},
   year = {2016}
}

TY  - CONF
ID  - 1373961
AU  - Gettová, Lenka - Vetešníková Šimková, Andrea
PY  - 2016
TI  - De-novo development of microsatellite markers for Dactylogyrus vistulae
SN  - 9788021083738
KW  - Monogenea
KW  - Dactylogyrus
KW  - microsatellites
N2  - In our study, we aimed to characterize de-novo microsatellite markers for Dactylogyrus parasites. Dactylogyrus vistulae was selected as a model organism owing to its relatively large body size in comparison to the other members of this genus. Approximately 300 specimens of D. vistulae were collected from European chub Squalius cephalus of the Dyje River. Genomic DNA was isolated and subsequently prepared genomic libraries were subjected to Illumina MiSeq (provided by CEITEC, MU, Brno). QDD program (Meglécz et al. 2010) was used for selecting microsatellite markers and primer design. Following criteria used in Gettová et al. (2013) 70 sequences with target microsatellites were selected. Monogenean parasites, especially dactylogyrids, display a high level of host specificity. As a result genetic structure of monogenean parasites might be host-dependent. Microsatellite markers developed for Dactylogyrus parasites might, therefore, enable us to reconstruct the evolution history of their fish hosts. In addition, in the case of rare generalist Dactylogyrus parasites (such is D. vistulae), we expect that the microsatellites will represent a useful tool to analyse the structure of parasite populations parasitizing different host species and/or geographically isolated parasite populations across Europe.
ER  -

GETTOVÁ, Lenka a Andrea VETEŠNÍKOVÁ ŠIMKOVÁ. De-novo development of microsatellite markers for Dactylogyrus vistulae. In \textit{5th Workshop of the European Centre of IchtyoParasitology}. 2016. ISBN~978-80-210-8373-8.

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