ABTS and DPPH methods as a tool for studying antioxidant capacity of spring barley and malt

MAREČEK, Vít, Alexandr MIKYŠKA, David HAMPEL, Pavel ČEJKA, Jana NEUWIRTHOVÁ, Alexandra MALACHOVÁ a Radim CERKAL. ABTS and DPPH methods as a tool for studying antioxidant capacity of spring barley and malt. Journal of Cereal Science. London: Academic Press Inc., 2017, roč. 73, January 2017, s. 40-45. ISSN 0733-5210. Dostupné z: https://dx.doi.org/10.1016/j.jcs.2016.11.004.

Základní údaje

Originální název

ABTS and DPPH methods as a tool for studying antioxidant capacity of spring barley and malt

Název anglicky

ABTS and DPPH methods as a tool for studying antioxidant capacity of spring barley and malt

Autoři

MAREČEK, Vít (203 Česká republika), Alexandr MIKYŠKA (203 Česká republika), David HAMPEL (203 Česká republika), Pavel ČEJKA (203 Česká republika), Jana NEUWIRTHOVÁ (203 Česká republika, garant, domácí), Alexandra MALACHOVÁ (203 Česká republika) a Radim CERKAL (203 Česká republika)

Vydání

Journal of Cereal Science, London, Academic Press Inc. 2017, 0733-5210

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Další údaje

Jazyk

čeština

Typ výsledku

Článek v odborném periodiku

Obor

40101 Agriculture

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.302

Kód RIV

RIV/00216224:14110/17:00096220

Organizační jednotka

Lékařská fakulta

DOI

http://dx.doi.org/10.1016/j.jcs.2016.11.004

UT WoS

000395224800007

Klíčová slova anglicky

Barley; Malting; Trolox; Antioxidant capacity determination

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

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Anotace

V originále

Many analytical methods for antioxidant determination in foodstuffs and raw materials based on various principles have been published so far. However, not all of them are applicable to barley and malt. The results of total antioxidant capacity (TEAC) of barley and malt obtained with methods based on ability to eliminate radicals of 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH) were compared and statistically evaluated. The average TEAC of barley determined using ABTS and DPPH ranged from 2.1 to 2.5 micromol g-1 and from 1.2 to 1.7 micromol g-1, respectively. The TEAC ranges in malt were 2.7-3.0 micromol g-1 (ABTS) and 1.8-2.6 micromol g-1 (DPPH). TEAC of barley and malt were affected by the weather conditions (year), variety and application of Zn2+ fertilizer. The ABTS and the DPPH methods represent an effective tool for the assessment of antioxidant capacity of spring barley and malt and the factors having an impact on it.

Anglicky

Many analytical methods for antioxidant determination in foodstuffs and raw materials based on various principles have been published so far. However, not all of them are applicable to barley and malt. The results of total antioxidant capacity (TEAC) of barley and malt obtained with methods based on ability to eliminate radicals of 2,2'-azino-bis-3-ethylbenzothiazoline-6-sulfonic acid (ABTS) and 2,2-diphenyl-1-(2,4,6-trinitrophenyl) hydrazyl (DPPH) were compared and statistically evaluated. The average TEAC of barley determined using ABTS and DPPH ranged from 2.1 to 2.5 micromol g-1 and from 1.2 to 1.7 micromol g-1, respectively. The TEAC ranges in malt were 2.7-3.0 micromol g-1 (ABTS) and 1.8-2.6 micromol g-1 (DPPH). TEAC of barley and malt were affected by the weather conditions (year), variety and application of Zn2+ fertilizer. The ABTS and the DPPH methods represent an effective tool for the assessment of antioxidant capacity of spring barley and malt and the factors having an impact on it.