Painting of Arabidopsis Chromosomes with Chromosome-Specific
BAC Clones

J 2016

Painting of Arabidopsis Chromosomes with Chromosome-Specific BAC Clones

MANDÁKOVÁ, Terezie a Martin LYSÁK

Základní údaje

Originální název

Painting of Arabidopsis Chromosomes with Chromosome-Specific BAC Clones

Autoři

MANDÁKOVÁ, Terezie a Martin LYSÁK

Vydání

Current protocols in plant biology, John Wiley & Sons, 2016, 2379-8068

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

Genetika a molekulární biologie

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Kód RIV

RIV/00216224:14740/16:00093748

Organizační jednotka

Středoevropský technologický institut

DOI

https://doi.org/10.1002/cppb.20022

Klíčová slova anglicky

BAC FISH; Brassicaceae; chromosome painting; fluorescence in situ hybridization (FISH); nick translation; pachytene chromosomes

Štítky

rivok

Změněno: 28. 2. 2017 15:13, Mgr. Eva Špillingová

Anotace

V originále

Chromosome painting (CP) refers to fluorescence in situ hybridization (FISH) of chromosome-specific DNA probes to identify large chromosome regions, chromosome arms, and whole chromosomes. For CP and CCP (comparative chromosome painting) in plants, most often, contigs of chromosome-specific bacterial artificial chromosomes (BAC) from the species of origin or a related species are used as painting probes. CP enables visualization and tracing of particular chromosome regions and/or chromosomes throughout all mitotic and meiotic stages as well as the corresponding interphase chromosome territories. CCP enables identification of large-scale homeologous chromosome regions and chromosomes shared among two or more species. Here, a step-by-step protocol for carrying out CP in Arabidopsis thaliana (Arabidopsis) and CCP in other crucifer taxa based on the use of Arabidopsis chromosome-specific BAC contigs is described.

Návaznosti

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

MANDÁKOVÁ, Terezie a Martin LYSÁK. Painting of Arabidopsis Chromosomes with Chromosome-Specific BAC Clones. Current protocols in plant biology. John Wiley & Sons, 2016, roč. 4, č. 2, s. 359-371. ISSN 2379-8068. Dostupné z: https://doi.org/10.1002/cppb.20022.

@article{1374157,
   author = {Mandáková, Terezie and Lysák, Martin},
   article_number = {2},
   doi = {https://doi.org/10.1002/cppb.20022},
   keywords = {BAC FISH; Brassicaceae; chromosome painting; fluorescence in situ hybridization (FISH); nick translation; pachytene chromosomes},
   language = {eng},
   issn = {2379-8068},
   journal = {Current protocols in plant biology},
   title = {Painting of Arabidopsis Chromosomes with Chromosome-Specific BAC Clones},
   url = {http://onlinelibrary.wiley.com/doi/10.1002/cppb.20022/abstract},
   volume = {4},
   year = {2016}
}

TY  - JOUR
ID  - 1374157
AU  - Mandáková, Terezie - Lysák, Martin
PY  - 2016
TI  - Painting of Arabidopsis Chromosomes with Chromosome-Specific BAC Clones
JF  - Current protocols in plant biology
VL  - 4
IS  - 2
SP  - 359-371
EP  - 359-371
PB  - John Wiley & Sons
SN  - 23798068
KW  - BAC FISH
KW  - Brassicaceae
KW  - chromosome painting
KW  - fluorescence in situ hybridization (FISH)
KW  - nick translation
KW  - pachytene chromosomes
UR  - http://onlinelibrary.wiley.com/doi/10.1002/cppb.20022/abstract
L2  - http://onlinelibrary.wiley.com/doi/10.1002/cppb.20022/abstract
N2  - Chromosome painting (CP) refers to fluorescence in situ hybridization (FISH) of chromosome-specific DNA probes to identify large chromosome regions, chromosome arms, and whole chromosomes. For CP and CCP (comparative chromosome painting) in plants, most often, contigs of chromosome-specific bacterial artificial chromosomes (BAC) from the species of origin or a related species are used as painting probes. CP enables visualization and tracing of particular chromosome regions and/or chromosomes throughout all mitotic and meiotic stages as well as the corresponding interphase chromosome territories. CCP enables identification of large-scale homeologous chromosome regions and chromosomes shared among two or more species. Here, a step-by-step protocol for carrying out CP in Arabidopsis thaliana (Arabidopsis) and CCP in other crucifer taxa based on the use of Arabidopsis chromosome-specific BAC contigs is described.
ER  -

MANDÁKOVÁ, Terezie a Martin LYSÁK. Painting of Arabidopsis Chromosomes with Chromosome-Specific BAC Clones. \textit{Current protocols in plant biology}. John Wiley \&{} Sons, 2016, roč.~4, č.~2, s.~359-371. ISSN~2379-8068. Dostupné z: https://doi.org/10.1002/cppb.20022.

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