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@article{1374157, author = {Mandáková, Terezie and Lysák, Martin}, article_number = {2}, doi = {http://dx.doi.org/10.1002/cppb.20022}, keywords = {BAC FISH; Brassicaceae; chromosome painting; fluorescence in situ hybridization (FISH); nick translation; pachytene chromosomes}, language = {eng}, issn = {2379-8068}, journal = {Current protocols in plant biology}, title = {Painting of Arabidopsis Chromosomes with Chromosome-Specific BAC Clones}, url = {http://onlinelibrary.wiley.com/doi/10.1002/cppb.20022/abstract}, volume = {4}, year = {2016} }
TY - JOUR ID - 1374157 AU - Mandáková, Terezie - Lysák, Martin PY - 2016 TI - Painting of Arabidopsis Chromosomes with Chromosome-Specific BAC Clones JF - Current protocols in plant biology VL - 4 IS - 2 SP - 359-371 EP - 359-371 PB - John Wiley & Sons SN - 23798068 KW - BAC FISH KW - Brassicaceae KW - chromosome painting KW - fluorescence in situ hybridization (FISH) KW - nick translation KW - pachytene chromosomes UR - http://onlinelibrary.wiley.com/doi/10.1002/cppb.20022/abstract L2 - http://onlinelibrary.wiley.com/doi/10.1002/cppb.20022/abstract N2 - Chromosome painting (CP) refers to fluorescence in situ hybridization (FISH) of chromosome-specific DNA probes to identify large chromosome regions, chromosome arms, and whole chromosomes. For CP and CCP (comparative chromosome painting) in plants, most often, contigs of chromosome-specific bacterial artificial chromosomes (BAC) from the species of origin or a related species are used as painting probes. CP enables visualization and tracing of particular chromosome regions and/or chromosomes throughout all mitotic and meiotic stages as well as the corresponding interphase chromosome territories. CCP enables identification of large-scale homeologous chromosome regions and chromosomes shared among two or more species. Here, a step-by-step protocol for carrying out CP in Arabidopsis thaliana (Arabidopsis) and CCP in other crucifer taxa based on the use of Arabidopsis chromosome-specific BAC contigs is described. ER -
MANDÁKOVÁ, Terezie a Martin LYSÁK. Painting of Arabidopsis Chromosomes with Chromosome-Specific BAC Clones. \textit{Current protocols in plant biology}. John Wiley \&{} Sons, 2016, roč.~4, č.~2, s.~359-371. ISSN~2379-8068. Dostupné z: https://dx.doi.org/10.1002/cppb.20022.
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