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@article{1374157,
author = {Mandáková, Terezie and Lysák, Martin},
article_number = {2},
doi = {http://dx.doi.org/10.1002/cppb.20022},
keywords = {BAC FISH; Brassicaceae; chromosome painting; fluorescence in situ hybridization (FISH); nick translation; pachytene chromosomes},
language = {eng},
issn = {2379-8068},
journal = {Current protocols in plant biology},
title = {Painting of Arabidopsis Chromosomes with Chromosome-Specific BAC Clones},
url = {http://onlinelibrary.wiley.com/doi/10.1002/cppb.20022/abstract},
volume = {4},
year = {2016}
}
TY - JOUR
ID - 1374157
AU - Mandáková, Terezie - Lysák, Martin
PY - 2016
TI - Painting of Arabidopsis Chromosomes with Chromosome-Specific BAC Clones
JF - Current protocols in plant biology
VL - 4
IS - 2
SP - 359-371
EP - 359-371
PB - John Wiley & Sons
SN - 23798068
KW - BAC FISH
KW - Brassicaceae
KW - chromosome painting
KW - fluorescence in situ hybridization (FISH)
KW - nick translation
KW - pachytene chromosomes
UR - http://onlinelibrary.wiley.com/doi/10.1002/cppb.20022/abstract
L2 - http://onlinelibrary.wiley.com/doi/10.1002/cppb.20022/abstract
N2 - Chromosome painting (CP) refers to fluorescence in situ hybridization (FISH) of chromosome-specific DNA probes to identify large chromosome regions, chromosome arms, and whole chromosomes. For CP and CCP (comparative chromosome painting) in plants, most often, contigs of chromosome-specific bacterial artificial chromosomes (BAC) from the species of origin or a related species are used as painting probes. CP enables visualization and tracing of particular chromosome regions and/or chromosomes throughout all mitotic and meiotic stages as well as the corresponding interphase chromosome territories. CCP enables identification of large-scale homeologous chromosome regions and chromosomes shared among two or more species. Here, a step-by-step protocol for carrying out CP in Arabidopsis thaliana (Arabidopsis) and CCP in other crucifer taxa based on the use of Arabidopsis chromosome-specific BAC contigs is described.
ER -
MANDÁKOVÁ, Terezie a Martin LYSÁK. Painting of Arabidopsis Chromosomes with Chromosome-Specific BAC Clones. \textit{Current protocols in plant biology}. John Wiley \&{} Sons, 2016, roč.~4, č.~2, s.~359-371. ISSN~2379-8068. Dostupné z: https://dx.doi.org/10.1002/cppb.20022.
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