The robust bio-immobilization based on pulsed plasma
polymerization of cyclopropylamine and glutaraldehyde coupling
chemistry

J 2016

The robust bio-immobilization based on pulsed plasma polymerization of cyclopropylamine and glutaraldehyde coupling chemistry

MANAKHOV, Anton; Ekaterina MAKHNEVA; Petr SKLÁDAL; David NEČAS; Jan CECHAL et al.

Základní údaje

Originální název

The robust bio-immobilization based on pulsed plasma polymerization of cyclopropylamine and glutaraldehyde coupling chemistry

Autoři

MANAKHOV, Anton; Ekaterina MAKHNEVA; Petr SKLÁDAL; David NEČAS; Jan CECHAL; Lukas KALINA; Marek ELIÁŠ a Lenka ZAJÍČKOVÁ

Vydání

Applied Surface Science, AMSTERDAM, Elsevier Science BV, 2016, 0169-4332

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10403 Physical chemistry

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 3.387

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/16:00093848

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

Amine coating; Sensors; Bio-immobilization; Plasma polymerization; Cyclopropylamine; XPS; QCM

Štítky

rivok

Změněno: 19. 9. 2017 04:54, doc. Mgr. Lenka Zajíčková, Ph.D.

Anotace

V originále

The performance of immunosensing devices crucially depends on the methodology of antibody or antigen immobilization on the sensor surface. Hence, the stable intermediate layers capable of specific and reproducible binding of antibodies are required. Herein, we introduce the amine rich (NHx concentration of 6 at.%) layers prepared by pulsed plasma polymerization of cyclopropylamine (CPA) for functionalization of the quartz crystal microbalance (QCM) surface by the antibody specific to human serum albumin. In these layers the amine groups serve as anchor for the antibody binding. The sensitivity of QCM sensors prepared in this way surpasses the one for the previously reported sensors functionalized by the thiolbased self-assembled monolayers by the factor of 2. Our results thus show that CPA plasma polymers have a significant potential for further development of the active layers for biosensing applications. (C) 2015 Elsevier B.V. All rights reserved.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

LM2011020, projekt VaV

Název: CEITEC ? open access

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC - open access

Citovat

MANAKHOV, Anton; Ekaterina MAKHNEVA; Petr SKLÁDAL; David NEČAS; Jan CECHAL; Lukas KALINA; Marek ELIÁŠ a Lenka ZAJÍČKOVÁ. The robust bio-immobilization based on pulsed plasma polymerization of cyclopropylamine and glutaraldehyde coupling chemistry. Applied Surface Science. AMSTERDAM: Elsevier Science BV, 2016, roč. 360, January, s. 28-36. ISSN 0169-4332. Dostupné z: https://doi.org/10.1016/j.apsusc.2015.10.178.

@article{1375041,
   author = {Manakhov, Anton and Makhneva, Ekaterina and Skládal, Petr and Nečas, David and Cechal, Jan and Kalina, Lukas and Eliáš, Marek and Zajíčková, Lenka},
   article_location = {AMSTERDAM},
   article_number = {January},
   doi = {https://doi.org/10.1016/j.apsusc.2015.10.178},
   keywords = {Amine coating; Sensors; Bio-immobilization; Plasma polymerization; Cyclopropylamine; XPS; QCM},
   language = {eng},
   issn = {0169-4332},
   journal = {Applied Surface Science},
   title = {The robust bio-immobilization based on pulsed plasma polymerization of cyclopropylamine and glutaraldehyde coupling chemistry},
   url = {http://www.sciencedirect.com/science/article/pii/S0169433215025970},
   volume = {360},
   year = {2016}
}

TY  - JOUR
ID  - 1375041
AU  - Manakhov, Anton - Makhneva, Ekaterina - Skládal, Petr - Nečas, David - Cechal, Jan - Kalina, Lukas - Eliáš, Marek - Zajíčková, Lenka
PY  - 2016
TI  - The robust bio-immobilization based on pulsed plasma polymerization of cyclopropylamine and glutaraldehyde coupling chemistry
JF  - Applied Surface Science
VL  - 360
IS  - January
SP  - 28-36
EP  - 28-36
PB  - Elsevier Science BV
SN  - 01694332
KW  - Amine coating
KW  - Sensors
KW  - Bio-immobilization
KW  - Plasma polymerization
KW  - Cyclopropylamine
KW  - XPS
KW  - QCM
UR  - http://www.sciencedirect.com/science/article/pii/S0169433215025970
L2  - http://www.sciencedirect.com/science/article/pii/S0169433215025970
N2  - The performance of immunosensing devices crucially depends on the methodology of antibody or antigen immobilization on the sensor surface. Hence, the stable intermediate layers capable of specific and reproducible binding of antibodies are required. Herein, we introduce the amine rich (NHx concentration of 6 at.%) layers prepared by pulsed plasma polymerization of cyclopropylamine (CPA) for functionalization of the quartz crystal microbalance (QCM) surface by the antibody specific to human serum albumin. In these layers the amine groups serve as anchor for the antibody binding. The sensitivity of QCM sensors prepared in this way surpasses the one for the previously reported sensors functionalized by the thiolbased self-assembled monolayers by the factor of 2. Our results thus show that CPA plasma polymers have a significant potential for further development of the active layers for biosensing applications. (C) 2015 Elsevier B.V. All rights reserved.
ER  -

MANAKHOV, Anton; Ekaterina MAKHNEVA; Petr SKLÁDAL; David NEČAS; Jan CECHAL; Lukas KALINA; Marek ELIÁŠ a Lenka ZAJÍČKOVÁ. The robust bio-immobilization based on pulsed plasma polymerization of cyclopropylamine and glutaraldehyde coupling chemistry. \textit{Applied Surface Science}. AMSTERDAM: Elsevier Science BV, 2016, roč.~360, January, s.~28-36. ISSN~0169-4332. Dostupné z: https://doi.org/10.1016/j.apsusc.2015.10.178.

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