The CD8+cell non-cytotoxic antiviral response affects RNA
polymerase II-mediated human immunodeficiency virus
transcription in infected CD4+cells

J 2016

The CD8+cell non-cytotoxic antiviral response affects RNA polymerase II-mediated human immunodeficiency virus transcription in infected CD4+cells

BLAŽEK, Dalibor; Fernando TEQUE; Carl MACKEWICZ; Matija PETERLIN; Jay A. LEVY et al.

Základní údaje

Originální název

The CD8+cell non-cytotoxic antiviral response affects RNA polymerase II-mediated human immunodeficiency virus transcription in infected CD4+cells

Autoři

BLAŽEK, Dalibor; Fernando TEQUE; Carl MACKEWICZ; Matija PETERLIN a Jay A. LEVY

Vydání

Journal of General Virology, Reading, Society of General Microbiology, 2016, 0022-1317

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.838

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/16:00088772

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

ANTI-HIV RESPONSE; CD8(+) T-CELLS; P-TEFB; FACTOR CAF; REPLICATION; INHIBITION; PATHOGENESIS; LYMPHOCYTES; ELONGATION; EXPRESSION

Štítky

rivok

Změněno: 14. 3. 2017 09:52, Mgr. Eva Špillingová

Anotace

V originále

A CD8+ cell non-cytotoxic antiviral response (CNAR), mediated by a CD8+ cell antiviral factor (CAF), is associated with a long-term healthy state in human immunodeficiency virus (HIV) infection. CNAR/CAF reduces viral transcription without a known effect on specific viral sequences in the HIV genome. In studies to define the mechanism involved in the block in viral transcription, we now report that transcription from the HIV-LTR reporter is reduced in infected CD4+ cells upon treatment with CAF. In agreement with this observation, the amount of RNA polymerase II (RNAPII) on the HIV promoter and other viral regions was strongly diminished in HIV-infected CD4+ cells co-cultivated with CNAR-expressing CD8+ cells. These results demonstrate further that CNAR/CAF has a specific role in regulating HIV transcription and a step during the preinitiation complex assembly appears to be sensitive to CNAR/CAF.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

GA14-09979S, projekt VaV

Název: Role Cdk12 a C-terminální domény RNA polymerázy II v transkripcně regulovaném procesu genomové nestability

Investor: Grantová agentura ČR, Role Cdk12 a C-terminální domény RNA polymerázy II v transkripcně regulovaném procesu genomové nestability

Citovat

BLAŽEK, Dalibor; Fernando TEQUE; Carl MACKEWICZ; Matija PETERLIN a Jay A. LEVY. The CD8+cell non-cytotoxic antiviral response affects RNA polymerase II-mediated human immunodeficiency virus transcription in infected CD4+cells. Journal of General Virology. Reading: Society of General Microbiology, 2016, roč. 97, January, s. 220-224. ISSN 0022-1317. Dostupné z: https://doi.org/10.1099/jgv.0.000326.

@article{1375560,
   author = {Blažek, Dalibor and Teque, Fernando and Mackewicz, Carl and Peterlin, Matija and Levy, Jay A.},
   article_location = {Reading},
   article_number = {January},
   doi = {https://doi.org/10.1099/jgv.0.000326},
   keywords = {ANTI-HIV RESPONSE; CD8(+) T-CELLS; P-TEFB; FACTOR CAF; REPLICATION; INHIBITION; PATHOGENESIS; LYMPHOCYTES; ELONGATION; EXPRESSION},
   language = {eng},
   issn = {0022-1317},
   journal = {Journal of General Virology},
   title = {The CD8+cell non-cytotoxic antiviral response affects RNA polymerase II-mediated human immunodeficiency virus transcription in infected CD4+cells},
   url = {http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000326},
   volume = {97},
   year = {2016}
}

TY  - JOUR
ID  - 1375560
AU  - Blažek, Dalibor - Teque, Fernando - Mackewicz, Carl - Peterlin, Matija - Levy, Jay A.
PY  - 2016
TI  - The CD8+cell non-cytotoxic antiviral response affects RNA polymerase II-mediated human immunodeficiency virus transcription in infected CD4+cells
JF  - Journal of General Virology
VL  - 97
IS  - January
SP  - 220-224
EP  - 220-224
PB  - Society of General Microbiology
SN  - 00221317
KW  - ANTI-HIV RESPONSE
KW  - CD8(+) T-CELLS
KW  - P-TEFB
KW  - FACTOR CAF
KW  - REPLICATION
KW  - INHIBITION
KW  - PATHOGENESIS
KW  - LYMPHOCYTES
KW  - ELONGATION
KW  - EXPRESSION
UR  - http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000326
L2  - http://jgv.microbiologyresearch.org/content/journal/jgv/10.1099/jgv.0.000326
N2  - A CD8+ cell non-cytotoxic antiviral response (CNAR), mediated by a CD8+ cell antiviral factor (CAF), is associated with a long-term healthy state in human immunodeficiency virus (HIV) infection. CNAR/CAF reduces viral transcription without a known effect on specific viral sequences in the HIV genome. In studies to define the mechanism involved in the block in viral transcription, we now report that transcription from the HIV-LTR reporter is reduced in infected CD4+ cells upon treatment with CAF. In agreement with this observation, the amount of RNA polymerase II (RNAPII) on the HIV promoter and other viral regions was strongly diminished in HIV-infected CD4+ cells co-cultivated with CNAR-expressing CD8+ cells. These results demonstrate further that CNAR/CAF has a specific role in regulating HIV transcription and a step during the preinitiation complex assembly appears to be sensitive to CNAR/CAF.
ER  -

BLAŽEK, Dalibor; Fernando TEQUE; Carl MACKEWICZ; Matija PETERLIN a Jay A. LEVY. The CD8+cell non-cytotoxic antiviral response affects RNA polymerase II-mediated human immunodeficiency virus transcription in infected CD4+cells. \textit{Journal of General Virology}. Reading: Society of General Microbiology, 2016, roč.~97, January, s.~220-224. ISSN~0022-1317. Dostupné z: https://doi.org/10.1099/jgv.0.000326.

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