Human Treponema pallidum 11q/j isolate belongs to subsp.
endemicum but contains two loci with a sequence in TP0548 and
TP0488 similar to subsp. pertenue and subsp. pallidum,
respectively

J 2017

Human Treponema pallidum 11q/j isolate belongs to subsp. endemicum but contains two loci with a sequence in TP0548 and TP0488 similar to subsp. pertenue and subsp. pallidum, respectively

MIKALOVÁ, Lenka; Michal STROUHAL; Jan OPPELT; Philippe Alain GRANGE; Michel JANIER et. al.

Basic information

Original name

Human Treponema pallidum 11q/j isolate belongs to subsp. endemicum but contains two loci with a sequence in TP0548 and TP0488 similar to subsp. pertenue and subsp. pallidum, respectively

Authors

MIKALOVÁ, Lenka (203 Czech Republic, belonging to the institution); Michal STROUHAL (203 Czech Republic, belonging to the institution); Jan OPPELT (203 Czech Republic, belonging to the institution); Philippe Alain GRANGE (250 France); Michel JANIER (250 France); Nadjet BENHADDOU (250 France); Nicolas DUPIN (250 France) and David ŠMAJS (203 Czech Republic, guarantor, belonging to the institution)

Edition

PLoS neglected tropical diseases, San Francisco, Public Library of Science, 2017, 1935-2735

Other information

Language

English

Type of outcome

Article in a journal

Field of Study

30303 Infectious Diseases

Country of publisher

United States of America

Confidentiality degree

is not subject to a state or trade secret

References:

URL

Impact factor

Impact factor: 4.367

RIV identification code

RIV/00216224:14110/17:00094671

Organization unit

Faculty of Medicine

DOI

http://dx.doi.org/10.1371/journal.pntd.0005434

UT WoS

000402251300033

EID Scopus

2-s2.0-85017025907

Keywords in English

Treponema pallidum

Abstract

In the original language

The TEN 11q/j isolate was characterized using nested PCR followed by Sanger sequencing and/or direct Illumina sequencing. Altogether, 44 chromosomal regions were analyzed. Overall, the 11q/j isolate clustered with TEN strains Bosnia A and Iraq B as expected from previous TEN classification of the 11q/j isolate. However, the 11q/j sequence in a 505 bp-long region at the TP0488 locus was similar to Treponema pallidum subsp. pallidum (TPA) strains, but not to TEN Bosnia A and Iraq B sequences, suggesting a recombination event at this locus. Similarly, the 11q/j sequence in a 613 bp-long region at the TP0548 locus was similar to Treponema pallidum subsp. pertenue (TPE) strains, but not to TEN sequences.

Links

GAP302/12/0574, research and development project

Name: Celogenomové sekvencování v analýze genomů a transkriptomů patogenních bakterií rodu Treponema

Investor: Czech Science Foundation

GA17-25455S, research and development project

Name: Studium genomů patogenních treponem na základě analýzy jednotlivých buněk

Investor: Czech Science Foundation

GP14-29596P, research and development project

Name: Genomová variabilita treponem během experimentální infekce

Investor: Czech Science Foundation

ROZV/20/LF/2015, interní kód MU

Name: LF - Příspěvek IP 2015

Investor: Ministry of Education, Youth and Sports of the CR

ROZV/24/LF/2016, interní kód MU

Name: LF - Příspěvek IP 2016

Investor: Ministry of Education, Youth and Sports of the CR

Cite

MIKALOVÁ, Lenka; Michal STROUHAL; Jan OPPELT; Philippe Alain GRANGE; Michel JANIER; Nadjet BENHADDOU; Nicolas DUPIN and David ŠMAJS. Human Treponema pallidum 11q/j isolate belongs to subsp. endemicum but contains two loci with a sequence in TP0548 and TP0488 similar to subsp. pertenue and subsp. pallidum, respectively. PLoS neglected tropical diseases. San Francisco: Public Library of Science, 2017, vol. 11, No 3, p. 1-14. ISSN 1935-2735. Available from: https://dx.doi.org/10.1371/journal.pntd.0005434.

@article{1375593,
   author = {Mikalová, Lenka and Strouhal, Michal and Oppelt, Jan and Grange, Philippe Alain and Janier, Michel and Benhaddou, Nadjet and Dupin, Nicolas and Šmajs, David},
   article_location = {San Francisco},
   article_number = {3},
   doi = {http://dx.doi.org/10.1371/journal.pntd.0005434},
   keywords = {Treponema pallidum},
   language = {eng},
   issn = {1935-2735},
   journal = {PLoS neglected tropical diseases},
   title = {Human Treponema pallidum 11q/j isolate belongs to subsp. endemicum but contains two loci with a sequence in TP0548 and TP0488 similar to subsp. pertenue and subsp. pallidum, respectively},
   url = {http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0005434},
   volume = {11},
   year = {2017}
}

TY  - JOUR
ID  - 1375593
AU  - Mikalová, Lenka - Strouhal, Michal - Oppelt, Jan - Grange, Philippe Alain - Janier, Michel - Benhaddou, Nadjet - Dupin, Nicolas - Šmajs, David
PY  - 2017
TI  - Human Treponema pallidum 11q/j isolate belongs to subsp. endemicum but contains two loci with a sequence in TP0548 and TP0488 similar to subsp. pertenue and subsp. pallidum, respectively
JF  - PLoS neglected tropical diseases
VL  - 11
IS  - 3
SP  - 1-14
EP  - 1-14
PB  - Public Library of Science
SN  - 19352735
KW  - Treponema pallidum
UR  - http://journals.plos.org/plosntds/article?id=10.1371/journal.pntd.0005434
N2  - The TEN 11q/j isolate was characterized using nested PCR followed by Sanger sequencing and/or direct Illumina sequencing. Altogether, 44 chromosomal regions were analyzed. Overall, the 11q/j isolate clustered with TEN strains Bosnia A and Iraq B as expected from previous TEN classification of the 11q/j isolate. However, the 11q/j sequence in a 505 bp-long region at the TP0488 locus was similar to Treponema pallidum subsp. pallidum (TPA) strains, but not to TEN Bosnia A and Iraq B sequences, suggesting a recombination event at this locus. Similarly, the 11q/j sequence in a 613 bp-long region at the TP0548 locus was similar to Treponema pallidum subsp. pertenue (TPE) strains, but not to TEN sequences.
ER  -

MIKALOVÁ, Lenka; Michal STROUHAL; Jan OPPELT; Philippe Alain GRANGE; Michel JANIER; Nadjet BENHADDOU; Nicolas DUPIN and David ŠMAJS. Human Treponema pallidum 11q/j isolate belongs to subsp. endemicum but contains two loci with a sequence in TP0548 and TP0488 similar to subsp. pertenue and subsp. pallidum, respectively. \textit{PLoS neglected tropical diseases}. San Francisco: Public Library of Science, 2017, vol.~11, No~3, p.~1-14. ISSN~1935-2735. Available from: https://dx.doi.org/10.1371/journal.pntd.0005434.

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