Differential Isotope Labeling of Glycopeptides for Accurate
Determination of Differences in Site-Specific Glycosylation

J 2016

Differential Isotope Labeling of Glycopeptides for Accurate Determination of Differences in Site-Specific Glycosylation

PABST, Martin; Iva BENEŠOVÁ; Stephan R FAGERER; Mathias JACOBSEN; Klaus EYER et al.

Základní údaje

Originální název

Differential Isotope Labeling of Glycopeptides for Accurate Determination of Differences in Site-Specific Glycosylation

Autoři

PABST, Martin; Iva BENEŠOVÁ; Stephan R FAGERER; Mathias JACOBSEN; Klaus EYER; Gregor SCHMIDT; Robert STEINHOFF; Jasmin KRISMER; Fabian WAHL; Jan PREISLER a Renato ZENOBI

Vydání

Journal of Proteome Research, Washington, American Chemical Society, 2016, 1535-3893

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10406 Analytical chemistry

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 4.268

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/16:00093930

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

glycopeptides; stable isotope labeling; IgG; MALDI MS; LC-ESI-MS

Štítky

AKR, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 2. 2018 22:03, prof. Mgr. Jan Preisler, Ph.D.

Anotace

V originále

We introduce a stable isotope labeling approach for glycopeptides that allows a specific glycosylation site in a protein to be quantitatively evaluated using mass spectrometry. Succinic anhydride is used to specifically label primary amino groups of the peptide portion of the glycopeptides. The heavy form ((D4C4)-C-13) provides an 8 Da mass increment over the light natural form ((H4C4)-C-12), allowing simultaneous analysis and direct comparison of two glycopeptide profiles in a single MS scan. We have optimized a protocol for an in-solution trypsin digestion, a one-pot labeling procedure, and a post-labeling solid-phase extraction to obtain purified and labeled glycopeptides. We provide the first demonstration of this approach by comparing IgGI Pc glycopeptides from polyclonal IgG samples with respect to their galactosylation and sialylation patterns using MALDI MS and LC-ESI-MS.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

EE2.3.30.0009, projekt VaV

Název: Zaměstnáním čerstvých absolventů doktorského studia k vědecké excelenci

Citovat

PABST, Martin; Iva BENEŠOVÁ; Stephan R FAGERER; Mathias JACOBSEN; Klaus EYER; Gregor SCHMIDT; Robert STEINHOFF; Jasmin KRISMER; Fabian WAHL; Jan PREISLER a Renato ZENOBI. Differential Isotope Labeling of Glycopeptides for Accurate Determination of Differences in Site-Specific Glycosylation. Journal of Proteome Research. Washington: American Chemical Society, 2016, roč. 15, č. 1, s. 326-331. ISSN 1535-3893. Dostupné z: https://doi.org/10.1021/acs.jproteome.5b00899.

@article{1375802,
   author = {Pabst, Martin and Benešová, Iva and Fagerer, Stephan R and Jacobsen, Mathias and Eyer, Klaus and Schmidt, Gregor and Steinhoff, Robert and Krismer, Jasmin and Wahl, Fabian and Preisler, Jan and Zenobi, Renato},
   article_location = {Washington},
   article_number = {1},
   doi = {https://doi.org/10.1021/acs.jproteome.5b00899},
   keywords = {glycopeptides; stable isotope labeling; IgG; MALDI MS; LC-ESI-MS},
   language = {eng},
   issn = {1535-3893},
   journal = {Journal of Proteome Research},
   title = {Differential Isotope Labeling of Glycopeptides for Accurate Determination of Differences in Site-Specific Glycosylation},
   volume = {15},
   year = {2016}
}

TY  - JOUR
ID  - 1375802
AU  - Pabst, Martin - Benešová, Iva - Fagerer, Stephan R - Jacobsen, Mathias - Eyer, Klaus - Schmidt, Gregor - Steinhoff, Robert - Krismer, Jasmin - Wahl, Fabian - Preisler, Jan - Zenobi, Renato
PY  - 2016
TI  - Differential Isotope Labeling of Glycopeptides for Accurate Determination of Differences in Site-Specific Glycosylation
JF  - Journal of Proteome Research
VL  - 15
IS  - 1
SP  - 326-331
EP  - 326-331
PB  - American Chemical Society
SN  - 15353893
KW  - glycopeptides
KW  - stable isotope labeling
KW  - IgG
KW  - MALDI MS
KW  - LC-ESI-MS
N2  - We introduce a stable isotope labeling approach for glycopeptides that allows a specific glycosylation site in a protein to be quantitatively evaluated using mass spectrometry. Succinic anhydride is used to specifically label primary amino groups of the peptide portion of the glycopeptides. The heavy form ((D4C4)-C-13) provides an 8 Da mass increment over the light natural form ((H4C4)-C-12), allowing simultaneous analysis and direct comparison of two glycopeptide profiles in a single MS scan. We have optimized a protocol for an in-solution trypsin digestion, a one-pot labeling procedure, and a post-labeling solid-phase extraction to obtain purified and labeled glycopeptides. We provide the first demonstration of this approach by comparing IgGI Pc glycopeptides from polyclonal IgG samples with respect to their galactosylation and sialylation patterns using MALDI MS and LC-ESI-MS.
ER  -

PABST, Martin; Iva BENEŠOVÁ; Stephan R FAGERER; Mathias JACOBSEN; Klaus EYER; Gregor SCHMIDT; Robert STEINHOFF; Jasmin KRISMER; Fabian WAHL; Jan PREISLER a Renato ZENOBI. Differential Isotope Labeling of Glycopeptides for Accurate Determination of Differences in Site-Specific Glycosylation. \textit{Journal of Proteome Research}. Washington: American Chemical Society, 2016, roč.~15, č.~1, s.~326-331. ISSN~1535-3893. Dostupné z: https://doi.org/10.1021/acs.jproteome.5b00899.

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