Mechanism for maturation-related reorganization of flavivirus
glycoproteins

J 2014

Mechanism for maturation-related reorganization of flavivirus glycoproteins

PLEVKA, Pavel; Anthony J. BATTISTI; Ju SHENG a Michael G. ROSSMANN

Základní údaje

Originální název

Mechanism for maturation-related reorganization of flavivirus glycoproteins

Autoři

PLEVKA, Pavel; Anthony J. BATTISTI; Ju SHENG a Michael G. ROSSMANN

Vydání

Journal of Structural Biology, UNITED STATES, ACADEMIC PRESS INC ELSEVIER SCIENCE, 2014, 1047-8477

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 3.231

Označené pro přenos do RIV

Organizační jednotka

Středoevropský technologický institut

DOI

https://doi.org/10.1016/j.jsb.2013.11.001

UT WoS

000330162300003

Klíčová slova anglicky

Flavivirus; Dengue; Maturation; Symmetry; Icosahedron; Mismatch

Štítky

neMU

Změněno: 29. 3. 2017 11:01, Mgr. Eva Špillingová

Anotace

V originále

Flaviviruses, such as dengue, West Nile, and yellow fever viruses, assemble as fusion-incompetent particles and subsequently undergo a large reorganization of their glycoprotein envelope resulting in formation of mature infectious virions. Here we used a combination of three-dimensional cryo-electron tomography and two-dimensional image analysis to study pleomorphic maturation intermediates of dengue virus 2. Icosahedral symmetries of immature and mature regions within one particle were mismatched relative to each other. Furthermore, the orientation of the two regions relative to each other differed among particles. Therefore, there cannot be a specific pathway determining the maturation of all particles. Instead, the region with mature structure expands when glycoproteins on its boundary acquire suitable orientation and conformation to allow them to become a stable part of the mature region. This type of maturation is possible because the envelope glycoproteins are anchored to the phospholipid bilayer that is a part of flavivirus virions and are thus restricted to movement on the two-dimensional surface of the particle. Therefore, compounds that limit movement of the glycoproteins within the virus membrane might be used as inhibitors of flavivirus maturation. (C) 2013 Elsevier Inc. All rights reserved.

Citovat

PLEVKA, Pavel; Anthony J. BATTISTI; Ju SHENG a Michael G. ROSSMANN. Mechanism for maturation-related reorganization of flavivirus glycoproteins. Journal of Structural Biology. UNITED STATES: ACADEMIC PRESS INC ELSEVIER SCIENCE, 2014, roč. 185, č. 1, s. 27-31. ISSN 1047-8477. Dostupné z: https://doi.org/10.1016/j.jsb.2013.11.001.

@article{1376792,
   author = {Plevka, Pavel and Battisti, Anthony J. and Sheng, Ju and Rossmann, Michael G.},
   article_location = {UNITED STATES},
   article_number = {1},
   doi = {https://doi.org/10.1016/j.jsb.2013.11.001},
   keywords = {Flavivirus; Dengue; Maturation; Symmetry; Icosahedron; Mismatch},
   language = {eng},
   issn = {1047-8477},
   journal = {Journal of Structural Biology},
   title = {Mechanism for maturation-related reorganization of flavivirus glycoproteins},
   volume = {185},
   year = {2014}
}

TY  - JOUR
ID  - 1376792
AU  - Plevka, Pavel - Battisti, Anthony J. - Sheng, Ju - Rossmann, Michael G.
PY  - 2014
TI  - Mechanism for maturation-related reorganization of flavivirus glycoproteins
JF  - Journal of Structural Biology
VL  - 185
IS  - 1
SP  - 27-31
EP  - 27-31
PB  - ACADEMIC PRESS INC ELSEVIER SCIENCE
SN  - 10478477
KW  - Flavivirus
KW  - Dengue
KW  - Maturation
KW  - Symmetry
KW  - Icosahedron
KW  - Mismatch
N2  - Flaviviruses, such as dengue, West Nile, and yellow fever viruses, assemble as fusion-incompetent particles and subsequently undergo a large reorganization of their glycoprotein envelope resulting in formation of mature infectious virions. Here we used a combination of three-dimensional cryo-electron tomography and two-dimensional image analysis to study pleomorphic maturation intermediates of dengue virus 2. Icosahedral symmetries of immature and mature regions within one particle were mismatched relative to each other. Furthermore, the orientation of the two regions relative to each other differed among particles. Therefore, there cannot be a specific pathway determining the maturation of all particles. Instead, the region with mature structure expands when glycoproteins on its boundary acquire suitable orientation and conformation to allow them to become a stable part of the mature region. This type of maturation is possible because the envelope glycoproteins are anchored to the phospholipid bilayer that is a part of flavivirus virions and are thus restricted to movement on the two-dimensional surface of the particle. Therefore, compounds that limit movement of the glycoproteins within the virus membrane might be used as inhibitors of flavivirus maturation. (C) 2013 Elsevier Inc. All rights reserved.
ER  -

PLEVKA, Pavel; Anthony J. BATTISTI; Ju SHENG a Michael G. ROSSMANN. Mechanism for maturation-related reorganization of flavivirus glycoproteins. \textit{Journal of Structural Biology}. UNITED STATES: ACADEMIC PRESS INC ELSEVIER SCIENCE, 2014, roč.~185, č.~1, s.~27-31. ISSN~1047-8477. Dostupné z: https://doi.org/10.1016/j.jsb.2013.11.001.

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