Rubella virus capsid protein structure and its role in virus
assembly and infection

J 2013

Rubella virus capsid protein structure and its role in virus assembly and infection

PRASAD, Vidya Mangala; Steven D. WILLOWS; Andrei FOKINE; Anthony J. BATTISTI; Siyang SUN et al.

Základní údaje

Originální název

Rubella virus capsid protein structure and its role in virus assembly and infection

Autoři

PRASAD, Vidya Mangala; Steven D. WILLOWS; Andrei FOKINE; Anthony J. BATTISTI; Siyang SUN; Pavel PLEVKA; Tom C. HOBMAN a Michael G. ROSSMANN

Vydání

Proceedings of the National Academy of Sciences of the United States of America, WASHINGTON, National Academy of Sciences, 2013, 0027-8424

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 9.809

Označené pro přenos do RIV

Organizační jednotka

Středoevropský technologický institut

DOI

https://doi.org/10.1073/pnas.1316681110

UT WoS

000328061700043

Klíčová slova anglicky

X-ray crystallography; cryoelectron tomography; virology

Štítky

neMU

Změněno: 29. 3. 2017 11:05, Mgr. Eva Špillingová

Anotace

V originále

Rubella virus (RV) is a leading cause of birth defects due to infectious agents. When contracted during pregnancy, RV infection leads to severe damage in fetuses. Despite its medical importance, compared with the related alphaviruses, very little is known about the structure of RV. The RV capsid protein is an essential structural component of virions as well as a key factor in virus-host interactions. Here we describe three crystal structures of the structural domain of the RV capsid protein. The polypeptide fold of the RV capsid protomer has not been observed previously. Combining the atomic structure of the RV capsid protein with the cryoelectron tomograms of RV particles established a low-resolution structure of the virion. Mutational studies based on this structure confirmed the role of amino acid residues in the capsid that function in the assembly of infectious virions.

Citovat

PRASAD, Vidya Mangala; Steven D. WILLOWS; Andrei FOKINE; Anthony J. BATTISTI; Siyang SUN; Pavel PLEVKA; Tom C. HOBMAN a Michael G. ROSSMANN. Rubella virus capsid protein structure and its role in virus assembly and infection. Proceedings of the National Academy of Sciences of the United States of America. WASHINGTON: National Academy of Sciences, 2013, roč. 110, č. 50, s. 20105-20110. ISSN 0027-8424. Dostupné z: https://doi.org/10.1073/pnas.1316681110.

@article{1376793,
   author = {Prasad, Vidya Mangala and Willows, Steven D. and Fokine, Andrei and Battisti, Anthony J. and Sun, Siyang and Plevka, Pavel and Hobman, Tom C. and Rossmann, Michael G.},
   article_location = {WASHINGTON},
   article_number = {50},
   doi = {https://doi.org/10.1073/pnas.1316681110},
   keywords = {X-ray crystallography; cryoelectron tomography; virology},
   language = {eng},
   issn = {0027-8424},
   journal = {Proceedings of the National Academy of Sciences of the United States of America},
   title = {Rubella virus capsid protein structure and its role in virus assembly and infection},
   volume = {110},
   year = {2013}
}

TY  - JOUR
ID  - 1376793
AU  - Prasad, Vidya Mangala - Willows, Steven D. - Fokine, Andrei - Battisti, Anthony J. - Sun, Siyang - Plevka, Pavel - Hobman, Tom C. - Rossmann, Michael G.
PY  - 2013
TI  - Rubella virus capsid protein structure and its role in virus assembly and infection
JF  - Proceedings of the National Academy of Sciences of the United States of America
VL  - 110
IS  - 50
SP  - 20105-20110
EP  - 20105-20110
PB  - National Academy of Sciences
SN  - 00278424
KW  - X-ray crystallography
KW  - cryoelectron tomography
KW  - virology
N2  - Rubella virus (RV) is a leading cause of birth defects due to infectious agents. When contracted during pregnancy, RV infection leads to severe damage in fetuses. Despite its medical importance, compared with the related alphaviruses, very little is known about the structure of RV. The RV capsid protein is an essential structural component of virions as well as a key factor in virus-host interactions. Here we describe three crystal structures of the structural domain of the RV capsid protein. The polypeptide fold of the RV capsid protomer has not been observed previously. Combining the atomic structure of the RV capsid protein with the cryoelectron tomograms of RV particles established a low-resolution structure of the virion. Mutational studies based on this structure confirmed the role of amino acid residues in the capsid that function in the assembly of infectious virions.
ER  -

PRASAD, Vidya Mangala; Steven D. WILLOWS; Andrei FOKINE; Anthony J. BATTISTI; Siyang SUN; Pavel PLEVKA; Tom C. HOBMAN a Michael G. ROSSMANN. Rubella virus capsid protein structure and its role in virus assembly and infection. \textit{Proceedings of the National Academy of Sciences of the United States of America}. WASHINGTON: National Academy of Sciences, 2013, roč.~110, č.~50, s.~20105-20110. ISSN~0027-8424. Dostupné z: https://doi.org/10.1073/pnas.1316681110.

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