PLEVKA, Pavel, Anthony J. BATTISTI, Dennis C. WINKLER, Kaspars TARS, Heather A. HOLDAWAY, Carol M. BATOR a Michael G. ROSSMANN. Sample Preparation Induced Artifacts in Cryo-Electron Tomographs. Microscopy and Microanalysis. Saarbrücken: Cambridge University Press, 2012, roč. 18, č. 5, s. 1043-1048. ISSN 1431-9276. Dostupné z: https://dx.doi.org/10.1017/S1431927612001298. |
Další formáty:
BibTeX
LaTeX
RIS
@article{1376810, author = {Plevka, Pavel and Battisti, Anthony J. and Winkler, Dennis C. and Tars, Kaspars and Holdaway, Heather A. and Bator, Carol M. and Rossmann, Michael G.}, article_location = {Saarbrücken}, article_number = {5}, doi = {http://dx.doi.org/10.1017/S1431927612001298}, keywords = {cryo-EM; microscopy; ice-vacuum interface; artifacts; virus; tomography}, language = {eng}, issn = {1431-9276}, journal = {Microscopy and Microanalysis}, title = {Sample Preparation Induced Artifacts in Cryo-Electron Tomographs}, volume = {18}, year = {2012} }
TY - JOUR ID - 1376810 AU - Plevka, Pavel - Battisti, Anthony J. - Winkler, Dennis C. - Tars, Kaspars - Holdaway, Heather A. - Bator, Carol M. - Rossmann, Michael G. PY - 2012 TI - Sample Preparation Induced Artifacts in Cryo-Electron Tomographs JF - Microscopy and Microanalysis VL - 18 IS - 5 SP - 1043-1048 EP - 1043-1048 PB - Cambridge University Press SN - 14319276 KW - cryo-EM KW - microscopy KW - ice-vacuum interface KW - artifacts KW - virus KW - tomography N2 - We investigated the effects of sample preparation and of the exposure to an electron beam on particles in cryo-electron tomographs. Various virus particles with icosahedral symmetry were examined, allowing a comparison of symmetrically related components that should be identical in structure but might be affected differently by these imaging artifacts. Comparison of tomographic reconstructions with previously determined structures established by an independent method showed that neither freezing nor electron beam exposure produced a significant amount of shrinkage along the z axis (thickness). However, we observed damage to regions of the particles located close to the surface of the vitreous ice. ER -
PLEVKA, Pavel, Anthony J. BATTISTI, Dennis C. WINKLER, Kaspars TARS, Heather A. HOLDAWAY, Carol M. BATOR a Michael G. ROSSMANN. Sample Preparation Induced Artifacts in Cryo-Electron Tomographs. \textit{Microscopy and Microanalysis}. Saarbrücken: Cambridge University Press, 2012, roč.~18, č.~5, s.~1043-1048. ISSN~1431-9276. Dostupné z: https://dx.doi.org/10.1017/S1431927612001298.
|