Cryo-Electron Tomography of Rubella Virus

J 2012

Cryo-Electron Tomography of Rubella Virus

BATTISTI, Anthony J.; Joshua D. YODER; Pavel PLEVKA; Dennis C. WINKLER; Vidya Mangala PRASAD et al.

Základní údaje

Originální název

Cryo-Electron Tomography of Rubella Virus

Autoři

BATTISTI, Anthony J.; Joshua D. YODER; Pavel PLEVKA; Dennis C. WINKLER; Vidya Mangala PRASAD; Richard J. KUHN; Teryl K. FREY; Alasdair C. STEVEN a Michael G. ROSSMANN

Vydání

JOURNAL OF VIROLOGY, WASHINGTON, AMER SOC MICROBIOLOGY, 2012, 0022-538X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 5.076

Označené pro přenos do RIV

Organizační jednotka

Středoevropský technologický institut

DOI

https://doi.org/10.1128/JVI.01390-12

UT WoS

000309114900015

Klíčová slova anglicky

CAPSID PROTEIN; ELECTRON-MICROSCOPY; SINDBIS VIRUS; NONSTRUCTURAL POLYPROTEIN; 3-DIMENSIONAL STRUCTURE; GLYCOPROTEIN; SEQUENCE; PARTICLES; E2; ALPHAVIRUSES

Štítky

neMU

Změněno: 29. 3. 2017 14:27, Mgr. Eva Špillingová

Anotace

V originále

Rubella virus is the only member of the Rubivirus genus within the Togaviridae family and is the causative agent of the childhood disease known as rubella or German measles. Here, we report the use of cryo-electron tomography to examine the three-dimensional structure of rubella virions and compare their structure to that of Ross River virus, a togavirus belonging the genus Alphavirus. The ectodomains of the rubella virus glycoproteins, E1 and E2, are shown to be organized into extended rows of density, separated by 9 nm on the viral surface. We also show that the rubella virus nucleocapsid structure often forms a roughly spherical shell which lacks high density at its center. While many rubella virions are approximately spherical and have dimensions similar to that of the icosahedral Ross River virus, the present results indicate that rubella exhibits a large degree of pleo-morphy. In addition, we used rotation function calculations and other analyses to show that approximately spherical rubella virions lack the icosahedral organization which characterizes Ross River and other alphaviruses. The present results indicate that the assembly mechanism of rubella virus, which has previously been shown to differ from that of the alphavirus assembly pathway, leads to an organization of the rubella virus structural proteins that is different from that of alphaviruses.

Citovat

BATTISTI, Anthony J.; Joshua D. YODER; Pavel PLEVKA; Dennis C. WINKLER; Vidya Mangala PRASAD; Richard J. KUHN; Teryl K. FREY; Alasdair C. STEVEN a Michael G. ROSSMANN. Cryo-Electron Tomography of Rubella Virus. JOURNAL OF VIROLOGY. WASHINGTON: AMER SOC MICROBIOLOGY, 2012, roč. 86, č. 20, s. 11078-11085. ISSN 0022-538X. Dostupné z: https://doi.org/10.1128/JVI.01390-12.

@article{1376811,
   author = {Battisti, Anthony J. and Yoder, Joshua D. and Plevka, Pavel and Winkler, Dennis C. and Prasad, Vidya Mangala and Kuhn, Richard J. and Frey, Teryl K. and Steven, Alasdair C. and Rossmann, Michael G.},
   article_location = {WASHINGTON},
   article_number = {20},
   doi = {https://doi.org/10.1128/JVI.01390-12},
   keywords = {CAPSID PROTEIN; ELECTRON-MICROSCOPY; SINDBIS VIRUS; NONSTRUCTURAL POLYPROTEIN; 3-DIMENSIONAL STRUCTURE; GLYCOPROTEIN; SEQUENCE; PARTICLES; E2; ALPHAVIRUSES},
   language = {eng},
   issn = {0022-538X},
   journal = {JOURNAL OF VIROLOGY},
   title = {Cryo-Electron Tomography of Rubella Virus},
   volume = {86},
   year = {2012}
}

TY  - JOUR
ID  - 1376811
AU  - Battisti, Anthony J. - Yoder, Joshua D. - Plevka, Pavel - Winkler, Dennis C. - Prasad, Vidya Mangala - Kuhn, Richard J. - Frey, Teryl K. - Steven, Alasdair C. - Rossmann, Michael G.
PY  - 2012
TI  - Cryo-Electron Tomography of Rubella Virus
JF  - JOURNAL OF VIROLOGY
VL  - 86
IS  - 20
SP  - 11078-11085
EP  - 11078-11085
PB  - AMER SOC MICROBIOLOGY
SN  - 0022538X
KW  - CAPSID PROTEIN
KW  - ELECTRON-MICROSCOPY
KW  - SINDBIS VIRUS
KW  - NONSTRUCTURAL POLYPROTEIN
KW  - 3-DIMENSIONAL STRUCTURE
KW  - GLYCOPROTEIN
KW  - SEQUENCE
KW  - PARTICLES
KW  - E2
KW  - ALPHAVIRUSES
N2  - Rubella virus is the only member of the Rubivirus genus within the Togaviridae family and is the causative agent of the childhood disease known as rubella or German measles. Here, we report the use of cryo-electron tomography to examine the three-dimensional structure of rubella virions and compare their structure to that of Ross River virus, a togavirus belonging the genus Alphavirus. The ectodomains of the rubella virus glycoproteins, E1 and E2, are shown to be organized into extended rows of density, separated by 9 nm on the viral surface. We also show that the rubella virus nucleocapsid structure often forms a roughly spherical shell which lacks high density at its center. While many rubella virions are approximately spherical and have dimensions similar to that of the icosahedral Ross River virus, the present results indicate that rubella exhibits a large degree of pleo-morphy. In addition, we used rotation function calculations and other analyses to show that approximately spherical rubella virions lack the icosahedral organization which characterizes Ross River and other alphaviruses. The present results indicate that the assembly mechanism of rubella virus, which has previously been shown to differ from that of the alphavirus assembly pathway, leads to an organization of the rubella virus structural proteins that is different from that of alphaviruses.
ER  -

BATTISTI, Anthony J.; Joshua D. YODER; Pavel PLEVKA; Dennis C. WINKLER; Vidya Mangala PRASAD; Richard J. KUHN; Teryl K. FREY; Alasdair C. STEVEN a Michael G. ROSSMANN. Cryo-Electron Tomography of Rubella Virus. \textit{JOURNAL OF VIROLOGY}. WASHINGTON: AMER SOC MICROBIOLOGY, 2012, roč.~86, č.~20, s.~11078-11085. ISSN~0022-538X. Dostupné z: https://doi.org/10.1128/JVI.01390-12.

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