Structure of a Packaging-Defective Mutant of Minute Virus of
Mice Indicates that the Genome Is Packaged via a Pore at a
5-Fold Axis

J 2011

Structure of a Packaging-Defective Mutant of Minute Virus of Mice Indicates that the Genome Is Packaged via a Pore at a 5-Fold Axis

PLEVKA, Pavel; Susan HAFENSTEIN; Lei LI; Anthony, Jr. D'ABRAMO; Susan F. COTMORE et al.

Základní údaje

Originální název

Structure of a Packaging-Defective Mutant of Minute Virus of Mice Indicates that the Genome Is Packaged via a Pore at a 5-Fold Axis

Autoři

PLEVKA, Pavel; Susan HAFENSTEIN; Lei LI; Anthony, Jr. D'ABRAMO; Susan F. COTMORE; Michael G. ROSSMANN a Peter TATTERSALL

Vydání

JOURNAL OF VIROLOGY, WASHINGTON, AMER SOC MICROBIOLOGY, 2011, 0022-538X

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 5.402

Označené pro přenos do RIV

Organizační jednotka

Středoevropský technologický institut

DOI

https://doi.org/10.1128/JVI.02598-10

UT WoS

000289787300019

Klíčová slova anglicky

VP1 N-TERMINUS; FUNCTIONAL IMPLICATIONS; CANINE PARVOVIRUS; DNA-REPLICATION; TYPE-2; PROTEIN; HELICASE; BINDING; CAPSIDS; VIRION

Štítky

neMU

Změněno: 29. 3. 2017 14:59, Mgr. Eva Špillingová

Anotace

V originále

The parvovirus minute virus of mice (MVM) packages a single copy of its linear single-stranded DNA genome into preformed capsids, in a process that is probably driven by a virus-encoded helicase. Parvoviruses have a roughly cylindrically shaped pore that surrounds each of the 12 5-fold vertices. The pore, which penetrates the virion shell, is created by the juxtaposition of 10 antiparallel beta-strands, two from each of the 5-fold-related capsid proteins. There is a bottleneck in the channel formed by the symmetry-related side chains of the leucines at position 172. We report here the X-ray crystal structure of the particles produced by a leucine-to-tryptophan mutation at position 172 and the analysis of its biochemical properties. The mutant capsid had its 5-fold channel blocked, and the particles were unable to package DNA, strongly suggesting that the 5-fold pore is the packaging portal for genome entry.

Citovat

PLEVKA, Pavel; Susan HAFENSTEIN; Lei LI; Anthony, Jr. D'ABRAMO; Susan F. COTMORE; Michael G. ROSSMANN a Peter TATTERSALL. Structure of a Packaging-Defective Mutant of Minute Virus of Mice Indicates that the Genome Is Packaged via a Pore at a 5-Fold Axis. JOURNAL OF VIROLOGY. WASHINGTON: AMER SOC MICROBIOLOGY, 2011, roč. 85, č. 10, s. 4822-4827. ISSN 0022-538X. Dostupné z: https://doi.org/10.1128/JVI.02598-10.

@article{1376819,
   author = {Plevka, Pavel and Hafenstein, Susan and Li, Lei and D'Abramo, Anthony, Jr. and Cotmore, Susan F. and Rossmann, Michael G. and Tattersall, Peter},
   article_location = {WASHINGTON},
   article_number = {10},
   doi = {https://doi.org/10.1128/JVI.02598-10},
   keywords = {VP1 N-TERMINUS; FUNCTIONAL IMPLICATIONS; CANINE PARVOVIRUS; DNA-REPLICATION; TYPE-2; PROTEIN; HELICASE; BINDING; CAPSIDS; VIRION},
   language = {eng},
   issn = {0022-538X},
   journal = {JOURNAL OF VIROLOGY},
   title = {Structure of a Packaging-Defective Mutant of Minute Virus of Mice Indicates that the Genome Is Packaged via a Pore at a 5-Fold Axis},
   volume = {85},
   year = {2011}
}

TY  - JOUR
ID  - 1376819
AU  - Plevka, Pavel - Hafenstein, Susan - Li, Lei - D'Abramo, Anthony, Jr. - Cotmore, Susan F. - Rossmann, Michael G. - Tattersall, Peter
PY  - 2011
TI  - Structure of a Packaging-Defective Mutant of Minute Virus of Mice Indicates that the Genome Is Packaged via a Pore at a 5-Fold Axis
JF  - JOURNAL OF VIROLOGY
VL  - 85
IS  - 10
SP  - 4822-4827
EP  - 4822-4827
PB  - AMER SOC MICROBIOLOGY
SN  - 0022538X
KW  - VP1 N-TERMINUS
KW  - FUNCTIONAL IMPLICATIONS
KW  - CANINE PARVOVIRUS
KW  - DNA-REPLICATION
KW  - TYPE-2
KW  - PROTEIN
KW  - HELICASE
KW  - BINDING
KW  - CAPSIDS
KW  - VIRION
N2  - The parvovirus minute virus of mice (MVM) packages a single copy of its linear single-stranded DNA genome into preformed capsids, in a process that is probably driven by a virus-encoded helicase. Parvoviruses have a roughly cylindrically shaped pore that surrounds each of the 12 5-fold vertices. The pore, which penetrates the virion shell, is created by the juxtaposition of 10 antiparallel beta-strands, two from each of the 5-fold-related capsid proteins. There is a bottleneck in the channel formed by the symmetry-related side chains of the leucines at position 172. We report here the X-ray crystal structure of the particles produced by a leucine-to-tryptophan mutation at position 172 and the analysis of its biochemical properties. The mutant capsid had its 5-fold channel blocked, and the particles were unable to package DNA, strongly suggesting that the 5-fold pore is the packaging portal for genome entry.
ER  -

PLEVKA, Pavel; Susan HAFENSTEIN; Lei LI; Anthony, Jr. D'ABRAMO; Susan F. COTMORE; Michael G. ROSSMANN a Peter TATTERSALL. Structure of a Packaging-Defective Mutant of Minute Virus of Mice Indicates that the Genome Is Packaged via a Pore at a 5-Fold Axis. \textit{JOURNAL OF VIROLOGY}. WASHINGTON: AMER SOC MICROBIOLOGY, 2011, roč.~85, č.~10, s.~4822-4827. ISSN~0022-538X. Dostupné z: https://doi.org/10.1128/JVI.02598-10.

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