| PLEVKA, Pavel, Susan HAFENSTEIN, Lei LI, Anthony, Jr. D'ABRAMO, Susan F. COTMORE, Michael G. ROSSMANN a Peter TATTERSALL. Structure of a Packaging-Defective Mutant of Minute Virus of Mice Indicates that the Genome Is Packaged via a Pore at a 5-Fold Axis. JOURNAL OF VIROLOGY. WASHINGTON: AMER SOC MICROBIOLOGY, 2011, roč. 85, č. 10, s. 4822-4827. ISSN 0022-538X. Dostupné z: https://dx.doi.org/10.1128/JVI.02598-10. |
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@article{1376819,
author = {Plevka, Pavel and Hafenstein, Susan and Li, Lei and D'Abramo, Anthony, Jr. and Cotmore, Susan F. and Rossmann, Michael G. and Tattersall, Peter},
article_location = {WASHINGTON},
article_number = {10},
doi = {http://dx.doi.org/10.1128/JVI.02598-10},
keywords = {VP1 N-TERMINUS; FUNCTIONAL IMPLICATIONS; CANINE PARVOVIRUS; DNA-REPLICATION; TYPE-2; PROTEIN; HELICASE; BINDING; CAPSIDS; VIRION},
language = {eng},
issn = {0022-538X},
journal = {JOURNAL OF VIROLOGY},
title = {Structure of a Packaging-Defective Mutant of Minute Virus of Mice Indicates that the Genome Is Packaged via a Pore at a 5-Fold Axis},
volume = {85},
year = {2011}
}
TY - JOUR
ID - 1376819
AU - Plevka, Pavel - Hafenstein, Susan - Li, Lei - D'Abramo, Anthony, Jr. - Cotmore, Susan F. - Rossmann, Michael G. - Tattersall, Peter
PY - 2011
TI - Structure of a Packaging-Defective Mutant of Minute Virus of Mice Indicates that the Genome Is Packaged via a Pore at a 5-Fold Axis
JF - JOURNAL OF VIROLOGY
VL - 85
IS - 10
SP - 4822-4827
EP - 4822-4827
PB - AMER SOC MICROBIOLOGY
SN - 0022538X
KW - VP1 N-TERMINUS
KW - FUNCTIONAL IMPLICATIONS
KW - CANINE PARVOVIRUS
KW - DNA-REPLICATION
KW - TYPE-2
KW - PROTEIN
KW - HELICASE
KW - BINDING
KW - CAPSIDS
KW - VIRION
N2 - The parvovirus minute virus of mice (MVM) packages a single copy of its linear single-stranded DNA genome into preformed capsids, in a process that is probably driven by a virus-encoded helicase. Parvoviruses have a roughly cylindrically shaped pore that surrounds each of the 12 5-fold vertices. The pore, which penetrates the virion shell, is created by the juxtaposition of 10 antiparallel beta-strands, two from each of the 5-fold-related capsid proteins. There is a bottleneck in the channel formed by the symmetry-related side chains of the leucines at position 172. We report here the X-ray crystal structure of the particles produced by a leucine-to-tryptophan mutation at position 172 and the analysis of its biochemical properties. The mutant capsid had its 5-fold channel blocked, and the particles were unable to package DNA, strongly suggesting that the 5-fold pore is the packaging portal for genome entry.
ER -
PLEVKA, Pavel, Susan HAFENSTEIN, Lei LI, Anthony, Jr. D'ABRAMO, Susan F. COTMORE, Michael G. ROSSMANN a Peter TATTERSALL. Structure of a Packaging-Defective Mutant of Minute Virus of Mice Indicates that the Genome Is Packaged via a Pore at a 5-Fold Axis. \textit{JOURNAL OF VIROLOGY}. WASHINGTON: AMER SOC MICROBIOLOGY, 2011, roč.~85, č.~10, s.~4822-4827. ISSN~0022-538X. Dostupné z: https://dx.doi.org/10.1128/JVI.02598-10.
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