Structure of a Packaging-Defective Mutant of Minute Virus of
Mice Indicates that the Genome Is Packaged via a Pore at a
5-Fold Axis

J 2011

Structure of a Packaging-Defective Mutant of Minute Virus of Mice Indicates that the Genome Is Packaged via a Pore at a 5-Fold Axis

PLEVKA, Pavel, Susan HAFENSTEIN, Lei LI, Anthony, Jr. D'ABRAMO, Susan F. COTMORE et. al.

Basic information

Original name

Structure of a Packaging-Defective Mutant of Minute Virus of Mice Indicates that the Genome Is Packaged via a Pore at a 5-Fold Axis

Authors

PLEVKA, Pavel, Susan HAFENSTEIN, Lei LI, Anthony, Jr. D'ABRAMO, Susan F. COTMORE, Michael G. ROSSMANN and Peter TATTERSALL

Edition

JOURNAL OF VIROLOGY, WASHINGTON, AMER SOC MICROBIOLOGY, 2011, 0022-538X

Other information

Language

English

Type of outcome

Článek v odborném periodiku

Field of Study

10600 1.6 Biological sciences

Country of publisher

United States of America

Confidentiality degree

není předmětem státního či obchodního tajemství

Impact factor

Impact factor: 5.402

Organization unit

Central European Institute of Technology

DOI

http://dx.doi.org/10.1128/JVI.02598-10

UT WoS

000289787300019

Keywords in English

VP1 N-TERMINUS; FUNCTIONAL IMPLICATIONS; CANINE PARVOVIRUS; DNA-REPLICATION; TYPE-2; PROTEIN; HELICASE; BINDING; CAPSIDS; VIRION

Abstract

V originále

The parvovirus minute virus of mice (MVM) packages a single copy of its linear single-stranded DNA genome into preformed capsids, in a process that is probably driven by a virus-encoded helicase. Parvoviruses have a roughly cylindrically shaped pore that surrounds each of the 12 5-fold vertices. The pore, which penetrates the virion shell, is created by the juxtaposition of 10 antiparallel beta-strands, two from each of the 5-fold-related capsid proteins. There is a bottleneck in the channel formed by the symmetry-related side chains of the leucines at position 172. We report here the X-ray crystal structure of the particles produced by a leucine-to-tryptophan mutation at position 172 and the analysis of its biochemical properties. The mutant capsid had its 5-fold channel blocked, and the particles were unable to package DNA, strongly suggesting that the 5-fold pore is the packaging portal for genome entry.

Citovat

PLEVKA, Pavel, Susan HAFENSTEIN, Lei LI, Anthony, Jr. D'ABRAMO, Susan F. COTMORE, Michael G. ROSSMANN and Peter TATTERSALL. Structure of a Packaging-Defective Mutant of Minute Virus of Mice Indicates that the Genome Is Packaged via a Pore at a 5-Fold Axis. JOURNAL OF VIROLOGY. WASHINGTON: AMER SOC MICROBIOLOGY, 2011, vol. 85, No 10, p. 4822-4827. ISSN 0022-538X. Available from: https://dx.doi.org/10.1128/JVI.02598-10.

@article{1376819,
   author = {Plevka, Pavel and Hafenstein, Susan and Li, Lei and D'Abramo, Anthony, Jr. and Cotmore, Susan F. and Rossmann, Michael G. and Tattersall, Peter},
   article_location = {WASHINGTON},
   article_number = {10},
   doi = {http://dx.doi.org/10.1128/JVI.02598-10},
   keywords = {VP1 N-TERMINUS; FUNCTIONAL IMPLICATIONS; CANINE PARVOVIRUS; DNA-REPLICATION; TYPE-2; PROTEIN; HELICASE; BINDING; CAPSIDS; VIRION},
   language = {eng},
   issn = {0022-538X},
   journal = {JOURNAL OF VIROLOGY},
   title = {Structure of a Packaging-Defective Mutant of Minute Virus of Mice Indicates that the Genome Is Packaged via a Pore at a 5-Fold Axis},
   volume = {85},
   year = {2011}
}

TY  - JOUR
ID  - 1376819
AU  - Plevka, Pavel - Hafenstein, Susan - Li, Lei - D'Abramo, Anthony, Jr. - Cotmore, Susan F. - Rossmann, Michael G. - Tattersall, Peter
PY  - 2011
TI  - Structure of a Packaging-Defective Mutant of Minute Virus of Mice Indicates that the Genome Is Packaged via a Pore at a 5-Fold Axis
JF  - JOURNAL OF VIROLOGY
VL  - 85
IS  - 10
SP  - 4822-4827
EP  - 4822-4827
PB  - AMER SOC MICROBIOLOGY
SN  - 0022538X
KW  - VP1 N-TERMINUS
KW  - FUNCTIONAL IMPLICATIONS
KW  - CANINE PARVOVIRUS
KW  - DNA-REPLICATION
KW  - TYPE-2
KW  - PROTEIN
KW  - HELICASE
KW  - BINDING
KW  - CAPSIDS
KW  - VIRION
N2  - The parvovirus minute virus of mice (MVM) packages a single copy of its linear single-stranded DNA genome into preformed capsids, in a process that is probably driven by a virus-encoded helicase. Parvoviruses have a roughly cylindrically shaped pore that surrounds each of the 12 5-fold vertices. The pore, which penetrates the virion shell, is created by the juxtaposition of 10 antiparallel beta-strands, two from each of the 5-fold-related capsid proteins. There is a bottleneck in the channel formed by the symmetry-related side chains of the leucines at position 172. We report here the X-ray crystal structure of the particles produced by a leucine-to-tryptophan mutation at position 172 and the analysis of its biochemical properties. The mutant capsid had its 5-fold channel blocked, and the particles were unable to package DNA, strongly suggesting that the 5-fold pore is the packaging portal for genome entry.
ER  -

PLEVKA, Pavel, Susan HAFENSTEIN, Lei LI, Anthony, Jr. D'ABRAMO, Susan F. COTMORE, Michael G. ROSSMANN and Peter TATTERSALL. Structure of a Packaging-Defective Mutant of Minute Virus of Mice Indicates that the Genome Is Packaged via a Pore at a 5-Fold Axis. \textit{JOURNAL OF VIROLOGY}. WASHINGTON: AMER SOC MICROBIOLOGY, 2011, vol.~85, No~10, p.~4822-4827. ISSN~0022-538X. Available from: https://dx.doi.org/10.1128/JVI.02598-10.

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