ROSSMANN, Michael G., Anthony J. BATTISTI a Pavel PLEVKA. FUTURE PROSPECTS. ADVANCES IN PROTEIN CHEMISTRY AND STRUCTURAL BIOLOGY, VOL 82: RECENT ADVANCES IN ELECTRON CRYOMICROSCOPY, PT B. SAN DIEGO: ELSEVIER ACADEMIC PRESS INC, 2011, roč. 82, neuvedeno, s. 101-121. ISSN 1876-1623. Dostupné z: https://dx.doi.org/10.1016/B978-0-12-386507-6.00005-1. |
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@article{1376825, author = {Rossmann, Michael G. and Battisti, Anthony J. and Plevka, Pavel}, article_location = {SAN DIEGO}, article_number = {neuvedeno}, doi = {http://dx.doi.org/10.1016/B978-0-12-386507-6.00005-1}, keywords = {3-DIMENSIONAL FOURIER SYNTHESIS; X-RAY ANALYSIS; CRYOELECTRON TOMOGRAPHY; ELECTRON-MICROSCOPY; ATOMIC MODELS; NEUTRALIZING ANTIBODY; INTERATOMIC DISTANCES; MOLECULAR-STRUCTURE; CRYSTAL-STRUCTURES; CANINE PARVOVIRUS}, language = {eng}, issn = {1876-1623}, journal = {ADVANCES IN PROTEIN CHEMISTRY AND STRUCTURAL BIOLOGY, VOL 82: RECENT ADVANCES IN ELECTRON CRYOMICROSCOPY, PT B}, title = {FUTURE PROSPECTS}, volume = {82}, year = {2011} }
TY - JOUR ID - 1376825 AU - Rossmann, Michael G. - Battisti, Anthony J. - Plevka, Pavel PY - 2011 TI - FUTURE PROSPECTS JF - ADVANCES IN PROTEIN CHEMISTRY AND STRUCTURAL BIOLOGY, VOL 82: RECENT ADVANCES IN ELECTRON CRYOMICROSCOPY, PT B VL - 82 IS - neuvedeno SP - 101-121 EP - 101-121 PB - ELSEVIER ACADEMIC PRESS INC SN - 18761623 KW - 3-DIMENSIONAL FOURIER SYNTHESIS KW - X-RAY ANALYSIS KW - CRYOELECTRON TOMOGRAPHY KW - ELECTRON-MICROSCOPY KW - ATOMIC MODELS KW - NEUTRALIZING ANTIBODY KW - INTERATOMIC DISTANCES KW - MOLECULAR-STRUCTURE KW - CRYSTAL-STRUCTURES KW - CANINE PARVOVIRUS N2 - Cryo-electron microscopy (cryo-EM) in combination with single-particle analysis has begun to complement crystallography in the study of large macromolecules at near-atomic resolution. Furthermore, advances in cryo-electron tomography have made possible the study of macromolecules within their cellular environment. Single-particle and tomographic studies will become even more useful when technologies for improving the signal-to-noise ratio such as direct electron detectors and phase plates become widely available. Automated image acquisition has significantly reduced the time and effort required to determine the structures of macromolecular assemblies. As a result, the number of structures determined by cryo-EM is growing exponentially. However, there is an urgent need for improved criteria for validating both the reconstruction process and the atomic models derived from cryo-EM data. Another major challenge will be mitigating the effects of anisotropy caused by the missing wedge and the excessively low signal-to-noise ratio for tomographic data. Parallels between the development of macromolecular crystallography and cryo-EM have been used to tentatively predict the future of cryo-EM. ER -
ROSSMANN, Michael G., Anthony J. BATTISTI a Pavel PLEVKA. FUTURE PROSPECTS. \textit{ADVANCES IN PROTEIN CHEMISTRY AND STRUCTURAL BIOLOGY, VOL 82: RECENT ADVANCES IN ELECTRON CRYOMICROSCOPY, PT B}. SAN DIEGO: ELSEVIER ACADEMIC PRESS INC, 2011, roč.~82, neuvedeno, s.~101-121. ISSN~1876-1623. Dostupné z: https://dx.doi.org/10.1016/B978-0-12-386507-6.00005-1.
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