Crystallization and preliminary X-ray diffraction analysis of
West Nile virus

J 2010

Crystallization and preliminary X-ray diffraction analysis of West Nile virus

KAUFMANN, Baerbel, Pavel PLEVKA, Richard J. KUHN a Michael G. ROSSMANN

Základní údaje

Originální název

Crystallization and preliminary X-ray diffraction analysis of West Nile virus

Autoři

KAUFMANN, Baerbel, Pavel PLEVKA, Richard J. KUHN a Michael G. ROSSMANN

Vydání

Acta crystallographica. Section F Structural biology and crystallization communications, HOBOKEN, WILEY-BLACKWELL, 2010, 1744-3091

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10600 1.6 Biological sciences

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 0.563

Organizační jednotka

Středoevropský technologický institut

DOI

http://dx.doi.org/10.1107/S1744309110009899

UT WoS

000277217700019

Klíčová slova anglicky

DENGUE VIRUS; ENVELOPE GLYCOPROTEIN; MEMBRANE-FUSION; VAPOR-DIFFUSION; PROTEIN; MATURATION; CRYSTALS

Štítky

neMU

Změněno: 30. 3. 2017 11:09, Mgr. Eva Špillingová

Anotace

V originále

West Nile virus, a human pathogen, is closely related to other medically important flaviviruses of global impact such as dengue virus. The infectious virus was purified from cell culture using polyethylene glycol (PEG) precipitation and density-gradient centrifugation. Thin amorphously shaped crystals of the lipid-enveloped virus were grown in quartz capillaries equilibrated by vapor diffusion. Crystal diffraction extended at best to a resolution of about 25 angstrom using synchrotron radiation. A preliminary analysis of the diffraction images indicated that the crystals had unit-cell parameters a similar or equal to b similar or equal to 480 angstrom, gamma = 120 degrees, suggesting a tight hexagonal packing of one virus particle per unit cell.

Citovat

KAUFMANN, Baerbel, Pavel PLEVKA, Richard J. KUHN a Michael G. ROSSMANN. Crystallization and preliminary X-ray diffraction analysis of West Nile virus. Acta crystallographica. Section F Structural biology and crystallization communications. HOBOKEN: WILEY-BLACKWELL, 2010, roč. 66, May, s. 558-562. ISSN 1744-3091. Dostupné z: https://dx.doi.org/10.1107/S1744309110009899.

@article{1376892,
   author = {Kaufmann, Baerbel and Plevka, Pavel and Kuhn, Richard J. and Rossmann, Michael G.},
   article_location = {HOBOKEN},
   article_number = {May},
   doi = {http://dx.doi.org/10.1107/S1744309110009899},
   keywords = {DENGUE VIRUS; ENVELOPE GLYCOPROTEIN; MEMBRANE-FUSION; VAPOR-DIFFUSION; PROTEIN; MATURATION; CRYSTALS},
   language = {eng},
   issn = {1744-3091},
   journal = {Acta crystallographica. Section F Structural biology and crystallization communications},
   title = {Crystallization and preliminary X-ray diffraction analysis of West Nile virus},
   volume = {66},
   year = {2010}
}

TY  - JOUR
ID  - 1376892
AU  - Kaufmann, Baerbel - Plevka, Pavel - Kuhn, Richard J. - Rossmann, Michael G.
PY  - 2010
TI  - Crystallization and preliminary X-ray diffraction analysis of West Nile virus
JF  - Acta crystallographica. Section F Structural biology and crystallization communications
VL  - 66
IS  - May
SP  - 558-562
EP  - 558-562
PB  - WILEY-BLACKWELL
SN  - 17443091
KW  - DENGUE VIRUS
KW  - ENVELOPE GLYCOPROTEIN
KW  - MEMBRANE-FUSION
KW  - VAPOR-DIFFUSION
KW  - PROTEIN
KW  - MATURATION
KW  - CRYSTALS
N2  - West Nile virus, a human pathogen, is closely related to other medically important flaviviruses of global impact such as dengue virus. The infectious virus was purified from cell culture using polyethylene glycol (PEG) precipitation and density-gradient centrifugation. Thin amorphously shaped crystals of the lipid-enveloped virus were grown in quartz capillaries equilibrated by vapor diffusion. Crystal diffraction extended at best to a resolution of about 25 angstrom using synchrotron radiation. A preliminary analysis of the diffraction images indicated that the crystals had unit-cell parameters a similar or equal to b similar or equal to 480 angstrom, gamma = 120 degrees, suggesting a tight hexagonal packing of one virus particle per unit cell.
ER  -

KAUFMANN, Baerbel, Pavel PLEVKA, Richard J. KUHN a Michael G. ROSSMANN. Crystallization and preliminary X-ray diffraction analysis of West Nile virus. \textit{Acta crystallographica. Section F Structural biology and crystallization communications}. HOBOKEN: WILEY-BLACKWELL, 2010, roč.~66, May, s.~558-562. ISSN~1744-3091. Dostupné z: https://dx.doi.org/10.1107/S1744309110009899.

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