Modulation of the Discriminatory Power of MALDI-TOF MS
Profiling for Distinguishing between Closely Related Bacterial
Strains

C 2016

Modulation of the Discriminatory Power of MALDI-TOF MS Profiling for Distinguishing between Closely Related Bacterial Strains

ŠEDO, Ondrej a Zbyněk ZDRÁHAL

Základní údaje

Originální název

Modulation of the Discriminatory Power of MALDI-TOF MS Profiling for Distinguishing between Closely Related Bacterial Strains

Autoři

ŠEDO, Ondrej a Zbyněk ZDRÁHAL

Vydání

Cham, Applications of Mass Spectrometry in Microbiology, od s. 185-202, 18 s. 2016

Nakladatel

Springer International Publishing Switzerland

Další údaje

Jazyk

angličtina

Typ výsledku

Kapitola resp. kapitoly v odborné knize

Obor

10406 Analytical chemistry

Stát vydavatele

Švýcarsko

Utajení

není předmětem státního či obchodního tajemství

Forma vydání

tištěná verze "print"

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/16:00094042

Organizační jednotka

Středoevropský technologický institut

ISBN

978-3-319-26068-6

DOI

https://doi.org/10.1007/978-3-319-26070-9_7

EID Scopus

2-s2.0-84960539447

Klíčová slova anglicky

MALDI-TOF MS; microbiology; bacteria identification

Štítky

rivok

Změněno: 27. 4. 2017 16:30, Mgr. Eva Špillingová

Anotace

V originále

The demands on MALDI-TOF MS profiling analyses for distinguishing between closely related bacterial strains are high compared to those for routine identification of bacterial species. Analytical success depends on detection of a significantly lower number of peaks, which requires a high degree of robustness in the method. For example, the influence of cultivation conditions was found to notably contribute to the discriminatory power of the method, as we have demonstrated in two examples concerning differentiation between strains of the Lactobacillus acidophilus group and selected Mycobacterium spp. The absence of specific signals in other examples concerning strains within the Acinetobacter calcoaceticus-Acinetobacter baumannii complex, Staphylococcus aureus, and Bacillus subtilis ecotypes led to the development of two alternative sample preparation protocols. By switching to an alternative matrix solution or by microwave-assisted tryptic digestion of the cellular material, these simple approaches enabled detection of new specific signals.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

Citovat

ŠEDO, Ondrej a Zbyněk ZDRÁHAL. Modulation of the Discriminatory Power of MALDI-TOF MS Profiling for Distinguishing between Closely Related Bacterial Strains. In Plamen Demirev. Applications of Mass Spectrometry in Microbiology. Cham: Springer International Publishing Switzerland, 2016, s. 185-202. ISBN 978-3-319-26068-6. Dostupné z: https://doi.org/10.1007/978-3-319-26070-9_7.

@inbook{1376923,
   author = {Šedo, Ondrej and Zdráhal, Zbyněk},
   address = {Cham},
   booktitle = {Applications of Mass Spectrometry in Microbiology},
   doi = {https://doi.org/10.1007/978-3-319-26070-9_7},
   editor = {Plamen Demirev},
   keywords = {MALDI-TOF MS; microbiology; bacteria identification},
   howpublished = {tištěná verze "print"},
   language = {eng},
   location = {Cham},
   isbn = {978-3-319-26068-6},
   pages = {185-202},
   publisher = {Springer International Publishing Switzerland},
   title = {Modulation of the Discriminatory Power of MALDI-TOF MS Profiling for Distinguishing between Closely Related Bacterial Strains},
   url = {http://link.springer.com/chapter/10.1007/978-3-319-26070-9_7},
   year = {2016}
}

TY  - CHAP
ID  - 1376923
AU  - Šedo, Ondrej - Zdráhal, Zbyněk
PY  - 2016
TI  - Modulation of the Discriminatory Power of MALDI-TOF MS Profiling for Distinguishing between Closely Related Bacterial Strains
VL  - Neuveden
PB  - Springer International Publishing Switzerland
CY  - Cham
SN  - 9783319260686
KW  - MALDI-TOF MS
KW  - microbiology
KW  - bacteria identification
UR  - http://link.springer.com/chapter/10.1007/978-3-319-26070-9_7
L2  - http://link.springer.com/chapter/10.1007/978-3-319-26070-9_7
N2  - The demands on MALDI-TOF MS profiling analyses for distinguishing between closely related bacterial strains are high compared to those for routine identification of bacterial species. Analytical success depends on detection of a significantly lower number of peaks, which requires a high degree of robustness in the method. For example, the influence of cultivation conditions was found to notably contribute to the discriminatory power of the method, as we have demonstrated in two examples concerning differentiation between strains of the Lactobacillus acidophilus group and selected Mycobacterium spp. The absence of specific signals in other examples concerning strains within the Acinetobacter calcoaceticus-Acinetobacter baumannii complex, Staphylococcus aureus, and Bacillus subtilis ecotypes led to the development of two alternative sample preparation protocols. By switching to an alternative matrix solution or by microwave-assisted tryptic digestion of the cellular material, these simple approaches enabled detection of new specific signals.
ER  -

ŠEDO, Ondrej a Zbyněk ZDRÁHAL. Modulation of the Discriminatory Power of MALDI-TOF MS Profiling for Distinguishing between Closely Related Bacterial Strains. In Plamen Demirev. \textit{Applications of Mass Spectrometry in Microbiology}. Cham: Springer International Publishing Switzerland, 2016, s.~185-202. ISBN~978-3-319-26068-6. Dostupné z: https://doi.org/10.1007/978-3-319-26070-9\_{}7.

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