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@article{1378631,
author = {Ciesiółka, Sylwia and Budna, Joanna and Jopek, Karol and Bryja, Artur and Kranc, Wiesława and Borys, Sylwia and Ješeta, Michal and Chachuła, Adrian and Ziółkowska, Agnieszka and Antosik, Paweł and Bukowska, Dorota and Brüssow, Klaus P. and Bruska, Małgorzata and Nowicki, Michał and Zabel, Maciej and Kempisty, Bartosz},
article_location = {New York},
article_number = {"neuvedeno"},
doi = {http://dx.doi.org/10.1155/2017/9738640},
keywords = {CELLULAR-DISTRIBUTION; CUMULUS CELLS; FOLLICULAR DEVELOPMENT; PROTEIN EXPRESSION; OOCYTE; PROGESTERONE; ESTROGEN; CONNEXIN-43; COMPETENCE; CX43},
language = {eng},
issn = {2314-6133},
journal = {Biomed Research International},
title = {Time- and Dose-Dependent Effects of 17 Beta-Estradiol on Short-Term, Real-Time Proliferation and Gene Expression in Porcine Granulosa Cells},
volume = {2017},
year = {2017}
}
TY - JOUR
ID - 1378631
AU - Ciesiółka, Sylwia - Budna, Joanna - Jopek, Karol - Bryja, Artur - Kranc, Wiesława - Borys, Sylwia - Ješeta, Michal - Chachuła, Adrian - Ziółkowska, Agnieszka - Antosik, Paweł - Bukowska, Dorota - Brüssow, Klaus P. - Bruska, Małgorzata - Nowicki, Michał - Zabel, Maciej - Kempisty, Bartosz
PY - 2017
TI - Time- and Dose-Dependent Effects of 17 Beta-Estradiol on Short-Term, Real-Time Proliferation and Gene Expression in Porcine Granulosa Cells
JF - Biomed Research International
VL - 2017
IS - "neuvedeno"
SP - 1-9
EP - 1-9
PB - Hindawi Publishing Corporation
SN - 23146133
KW - CELLULAR-DISTRIBUTION
KW - CUMULUS CELLS
KW - FOLLICULAR DEVELOPMENT
KW - PROTEIN EXPRESSION
KW - OOCYTE
KW - PROGESTERONE
KW - ESTROGEN
KW - CONNEXIN-43
KW - COMPETENCE
KW - CX43
N2 - The key mechanisms responsible for achievement of full reproductive and developmental capability in mammals are the differentiation and transformation of granulosa cells (GCs) during folliculogenesis, oogenesis, and oocytematuration. Although the role of 17 beta-estradiol (E2) in ovarian activity is widely known, its effect on proliferative capacity, gap junction connection (GJC) formation, andGCs-luteal cells transformation requires further research. Therefore, the goal of this study was to assess the real-time proliferative activity of porcine GCs in vitro in relation to connexin (Cx), luteinizing hormone receptor (LHR), follicle stimulating hormone receptor (FSHR), and aromatase (CYP19A1) expression during short-term(168 h) primary culture. The cultured GCs were exposed to acute (at 96 h of culture) and/or prolonged (between 0 and 168 h of culture) administration of 1.8 and 3.6.. mu M E2. The relative abundance of Cx36, Cx37, Cx40, Cx43, LHR, FSHR, and CYP19A1mRNA wasmeasured. We conclude that the proliferation capability of GCs in vitro is substantially associated with expression of Cxs, LHR, FSHR, andCYP19A1. Furthermore, the GC-luteal cell transformation in vitro may be significantly accompanied by the proliferative activity of GCs in pigs.
ER -
CIESIÓŁKA, Sylwia, Joanna BUDNA, Karol JOPEK, Artur BRYJA, Wiesława KRANC, Sylwia BORYS, Michal JEŠETA, Adrian CHACHUŁA, Agnieszka ZIÓŁKOWSKA, Paweł ANTOSIK, Dorota BUKOWSKA, Klaus P. BRÜSSOW, Małgorzata BRUSKA, Michał NOWICKI, Maciej ZABEL a Bartosz KEMPISTY. Time- and Dose-Dependent Effects of 17 Beta-Estradiol on Short-Term, Real-Time Proliferation and Gene Expression in Porcine Granulosa Cells. \textit{Biomed Research International}. New York: Hindawi Publishing Corporation, roč.~2017, ''neuvedeno'', s.~1-9. ISSN~2314-6133. doi:10.1155/2017/9738640. 2017.
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