Microscopic techniques used for the study of cell cortex
architecture in gregarines

a 2017

Microscopic techniques used for the study of cell cortex architecture in gregarines

KOVÁČIKOVÁ, Magdaléna, Naděžda VAŠKOVICOVÁ a Andrea BARDŮNEK VALIGUROVÁ

Základní údaje

Originální název

Microscopic techniques used for the study of cell cortex architecture in gregarines

Autoři

KOVÁČIKOVÁ, Magdaléna (703 Slovensko, garant, domácí), Naděžda VAŠKOVICOVÁ (203 Česká republika) a Andrea BARDŮNEK VALIGUROVÁ (703 Slovensko, domácí)

Vydání

MICROSCOPY 2017 conference, 2017

Další údaje

Jazyk

angličtina

Typ výsledku

Konferenční abstrakt

Obor

10600 1.6 Biological sciences

Stát vydavatele

Česká republika

Utajení

není předmětem státního či obchodního tajemství

Kód RIV

RIV/00216224:14310/17:00094741

Organizační jednotka

Přírodovědecká fakulta

Klíčová slova anglicky

Cell cortex; CLSM; Freeze etching; Gregarines; SEM; TEM

Změněno: 28. 8. 2017 10:17, Mgr. Magdaléna Kováčiková, Ph.D.

Anotace

V originále

Gregarines, members of the group Apicomplexa, represent unicellular organisms, parasitising wide range of invertebrate hosts. Their cell cortex consists of outer plasma membrane covering cell surface under which closely apposed alveoli (inner membrane complex; IMC) are located. Electron (scanning and transmission) and confocal microscopic analyses were performed to determine morphology, ultrastructural organisation and biochemical nature of cortical compartments in eugregarine Gregarina garnhami parasitising intestine of desert locust Schistocerca gregaria. Pellicle, covering the surface of gregarine, creates numerous epicytic folds arranged in longitudinal lines separated by grooves. At the bottom of the grooves, micropores (large-sized pores) together with medium-sized pores were detected. Moreover, the fracture faces of IMC revealed the presence of medium and small-sized pores in the lateral part of epicytic folds. Pores were distributed irregularly. Analysis of the supramolecular organisation of the plasma membrane and both cytomembranes performed by freeze-etching analysis showed that the density of intramembranous particles (IMPs) was high and their size varied in the range from 0.5 to 17.4 nm, depending on the membrane and its fractured face. Under the pellicle, the net of filamentous structures (ectoplasmic network and myonemes - F-actin of origin) occurred in perpendicular and circular orientation to longitudinal cell axis.

Návaznosti

GBP505/12/G112, projekt VaV

Název: ECIP - Evropské centrum ichtyoparazitologie

Investor: Grantová agentura ČR, ECIP - Evropské centrum ichtyoparazitologie

Citovat

KOVÁČIKOVÁ, Magdaléna, Naděžda VAŠKOVICOVÁ a Andrea BARDŮNEK VALIGUROVÁ. Microscopic techniques used for the study of cell cortex architecture in gregarines. In MICROSCOPY 2017 conference. 2017.

@proceedings{1380286,
   author = {Kováčiková, Magdaléna and Vaškovicová, Naděžda and Bardůnek Valigurová, Andrea},
   booktitle = {MICROSCOPY 2017 conference},
   keywords = {Cell cortex; CLSM; Freeze etching; Gregarines; SEM; TEM},
   language = {eng},
   title = {Microscopic techniques used for the study of cell cortex architecture in gregarines},
   year = {2017}
}

TY  - CONF
ID  - 1380286
AU  - Kováčiková, Magdaléna - Vaškovicová, Naděžda - Bardůnek Valigurová, Andrea
PY  - 2017
TI  - Microscopic techniques used for the study of cell cortex architecture in gregarines
KW  - Cell cortex
KW  - CLSM
KW  - Freeze etching
KW  - Gregarines
KW  - SEM
KW  - TEM
N2  - Gregarines, members of the group Apicomplexa, represent unicellular organisms, parasitising wide range of invertebrate hosts. Their cell cortex consists of outer plasma membrane covering cell surface under which closely apposed alveoli (inner membrane complex; IMC) are located. Electron (scanning and transmission) and confocal microscopic analyses were performed to determine morphology, ultrastructural organisation and biochemical nature of cortical compartments in eugregarine Gregarina garnhami parasitising intestine of desert locust Schistocerca gregaria. Pellicle, covering the surface of gregarine, creates numerous epicytic folds arranged in longitudinal lines separated by grooves. At the bottom of the grooves, micropores (large-sized pores) together with medium-sized pores were detected. Moreover, the fracture faces of IMC revealed the presence of medium and small-sized pores in the lateral part of epicytic folds. Pores were distributed irregularly. Analysis of the supramolecular organisation of the plasma membrane and both cytomembranes performed by freeze-etching analysis showed that the density of intramembranous particles (IMPs) was high and their size varied in the range from 0.5 to 17.4 nm, depending on the membrane and its fractured face. Under the pellicle, the net of filamentous structures (ectoplasmic network and myonemes - F-actin of origin) occurred in perpendicular and circular orientation to longitudinal cell axis.
ER  -

KOVÁČIKOVÁ, Magdaléna, Naděžda VAŠKOVICOVÁ a Andrea BARDŮNEK VALIGUROVÁ. Microscopic techniques used for the study of cell cortex architecture in gregarines. In \textit{MICROSCOPY 2017 conference}. 2017.

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