Global MicroRNA Expression Profiling Identifies Unique MicroRNA
Pattern of Radioresistant Glioblastoma Cells

J 2017

Global MicroRNA Expression Profiling Identifies Unique MicroRNA Pattern of Radioresistant Glioblastoma Cells

ONDRÁČEK, Jakub; Pavel FADRUS; Jiří ŠÁNA; Andrej BEŠŠE; Tomáš LOJA et al.

Základní údaje

Originální název

Global MicroRNA Expression Profiling Identifies Unique MicroRNA Pattern of Radioresistant Glioblastoma Cells

Autoři

ONDRÁČEK, Jakub; Pavel FADRUS; Jiří ŠÁNA; Andrej BEŠŠE; Tomáš LOJA; Marek VEČEŘA; Lenka RADOVÁ; Martin SMRČKA; Pavel ŠLAMPA a Ondřej SLABÝ

Vydání

Anticancer Research, Athens, The International Institute of Anticancer Research, 2017, 0250-7005

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30200 3.2 Clinical medicine

Stát vydavatele

Řecko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 1.865

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14740/17:00095642

Organizační jednotka

Středoevropský technologický institut

Klíčová slova anglicky

Glioblastoma; radioresistance; microRNA

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 28. 3. 2018 15:55, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Glioblastoma multiforme ( GBM) is the most aggressive intracranial tumor characterized with infaust prognosis. Despite advances in neurosurgical and radiotherapeutic techniques and chemotherapy, the median overall survival ranges between 12-15 months from diagnosis. The main cause of treatment failure is considered the presence of tumor cells resistant to conventional therapy, mainly radiotherapy. MicroRNAs (miRNAs) are small, non-coding RNAs that function as post-transcriptional regulators of gene expression and have been repeatedly proven to play important roles in pathogenesis and biological features of many cancers, including GBM and its radioresistant phenotype. In our study, we established radioresistant cells from the commonly used human GBM cell lines T98G, U87MG and U251. Consequently, we performed global miRNA expression profiling in both radioresistant and parental cell lines and identified 113 miRNAs with significantly different expression (p < 0.05) between these two groups (73 miRNAs were up-regulated, 40 miRNAs were down-regulated). Some of these miRNAs have been previously described in relation to ionizing radiation, and others were herein identified for the first time. We believe that after deeper functional investigation of identified miRNAs in relation to radioresistance, these miRNAs present potential predictive biomarkers or therapeutic targets in GBM.

Návaznosti

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

NV15-33158A, projekt VaV

Název: Nová úroveň molekulární taxonomie glioblastomu založená na expresních profilech dlouhých nekódujících RNA: implikace pro diagnostiku a terapii

NV15-34553A, projekt VaV

Název: Využití mikroRNA v mozkomíšním moku pro diagnostiku mozkových nádorů

Citovat

ONDRÁČEK, Jakub; Pavel FADRUS; Jiří ŠÁNA; Andrej BEŠŠE; Tomáš LOJA; Marek VEČEŘA; Lenka RADOVÁ; Martin SMRČKA; Pavel ŠLAMPA a Ondřej SLABÝ. Global MicroRNA Expression Profiling Identifies Unique MicroRNA Pattern of Radioresistant Glioblastoma Cells. Anticancer Research. Athens: The International Institute of Anticancer Research, 2017, roč. 37, č. 3, s. 1099-1104. ISSN 0250-7005. Dostupné z: https://doi.org/10.21873/anticanres.11422.

@article{1381998,
   author = {Ondráček, Jakub and Fadrus, Pavel and Šána, Jiří and Bešše, Andrej and Loja, Tomáš and Večeřa, Marek and Radová, Lenka and Smrčka, Martin and Šlampa, Pavel and Slabý, Ondřej},
   article_location = {Athens},
   article_number = {3},
   doi = {https://doi.org/10.21873/anticanres.11422},
   keywords = {Glioblastoma; radioresistance; microRNA},
   language = {eng},
   issn = {0250-7005},
   journal = {Anticancer Research},
   title = {Global MicroRNA Expression Profiling Identifies Unique MicroRNA Pattern of Radioresistant Glioblastoma Cells},
   url = {http://ar.iiarjournals.org/content/37/3/1099.abstract},
   volume = {37},
   year = {2017}
}

TY  - JOUR
ID  - 1381998
AU  - Ondráček, Jakub - Fadrus, Pavel - Šána, Jiří - Bešše, Andrej - Loja, Tomáš - Večeřa, Marek - Radová, Lenka - Smrčka, Martin - Šlampa, Pavel - Slabý, Ondřej
PY  - 2017
TI  - Global MicroRNA Expression Profiling Identifies Unique MicroRNA Pattern of Radioresistant Glioblastoma Cells
JF  - Anticancer Research
VL  - 37
IS  - 3
SP  - 1099-1104
EP  - 1099-1104
PB  - The International Institute of Anticancer Research
SN  - 02507005
KW  - Glioblastoma
KW  - radioresistance
KW  - microRNA
UR  - http://ar.iiarjournals.org/content/37/3/1099.abstract
L2  - http://ar.iiarjournals.org/content/37/3/1099.abstract
N2  - Glioblastoma multiforme ( GBM) is the most aggressive intracranial tumor characterized with infaust prognosis. Despite advances in neurosurgical and radiotherapeutic techniques and chemotherapy, the median overall survival ranges between 12-15 months from diagnosis. The main cause of treatment failure is considered the presence of tumor cells resistant to conventional therapy, mainly radiotherapy. MicroRNAs (miRNAs) are small, non-coding RNAs that function as post-transcriptional regulators of gene expression and have been repeatedly proven to play important roles in pathogenesis and biological features of many cancers, including GBM and its radioresistant phenotype. In our study, we established radioresistant cells from the commonly used human GBM cell lines T98G, U87MG and U251. Consequently, we performed global miRNA expression profiling in both radioresistant and parental cell lines and identified 113 miRNAs with significantly different expression (p < 0.05) between these two groups (73 miRNAs were up-regulated, 40 miRNAs were down-regulated). Some of these miRNAs have been previously described in relation to ionizing radiation, and others were herein identified for the first time. We believe that after deeper functional investigation of identified miRNAs in relation to radioresistance, these miRNAs present potential predictive biomarkers or therapeutic targets in GBM.
ER  -

ONDRÁČEK, Jakub; Pavel FADRUS; Jiří ŠÁNA; Andrej BEŠŠE; Tomáš LOJA; Marek VEČEŘA; Lenka RADOVÁ; Martin SMRČKA; Pavel ŠLAMPA a Ondřej SLABÝ. Global MicroRNA Expression Profiling Identifies Unique MicroRNA Pattern of Radioresistant Glioblastoma Cells. \textit{Anticancer Research}. Athens: The International Institute of Anticancer Research, 2017, roč.~37, č.~3, s.~1099-1104. ISSN~0250-7005. Dostupné z: https://doi.org/10.21873/anticanres.11422.

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