Propionibacterium acnes biofilm is present in intervertebral
discs of patients undergoing microdiscectomy

J 2017

Propionibacterium acnes biofilm is present in intervertebral discs of patients undergoing microdiscectomy

MANU NATH CAPOOR, externista; Filip RŮŽIČKA; Jonathan E. SCHMITZ; Garth A. JAMES; Táňa MACHÁČKOVÁ et. al.

Základní údaje

Originální název

Propionibacterium acnes biofilm is present in intervertebral discs of patients undergoing microdiscectomy

Autoři

Vydání

Plos one, San Francisco, Public Library of Science, 2017, 1932-6203

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30102 Immunology

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.766

Kód RIV

RIV/00216224:14740/17:00096860

Organizační jednotka

Středoevropský technologický institut

DOI

https://doi.org/10.1371/journal.pone.0174518

UT WoS

000399351000019

EID Scopus

2-s2.0-85016602260

Klíčová slova anglicky

LOW-BACK-PAIN; SAPHO SYNDROME; MODIC CHANGES; LUMBAR SPINE; SARCOIDOSIS; INFECTIONS; ASSOCIATION; CONTAMINATION; INVOLVEMENT; DIAGNOSIS

Štítky

OA, rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 17. 5. 2018 13:58, Mgr. Pavla Foltynová, Ph.D.

Anotace

V originále

Background In previous studies, Propionibacterium acnes was cultured from intervertebral disc tissue of similar to 25% of patients undergoing microdiscectomy, suggesting a possible link between chronic bacterial infection and disc degeneration. However, given the prominence of P. acnes as a skin commensal, such analyses often struggled to exclude the alternate possibility that these organisms represent perioperative microbiologic contamination. This investigation seeks to validate P. acnes prevalence in resected disc cultures, while providing microscopic evidence of P. acnes biofilm in the intervertebral discs. Methods Specimens from 368 patients undergoing microdiscectomy for disc herniation were divided into several fragments, one being homogenized, subjected to quantitative anaerobic culture, and assessed for bacterial growth, and a second fragment frozen for additional analyses. Colonies were identified by MALDI-TOF mass spectrometry and P. acnes phylotyping was conducted by multiplex PCR. For a sub-set of specimens, bacteria localization within the disc was assessed by microscopy using confocal laser scanning and FISH. Results Bacteria were cultured from 162 discs (44%), including 119 cases (32.3%) with P. acnes. In 89 cases, P. acnes was cultured exclusively; in 30 cases, it was isolated in combination with other bacteria (primarily coagulase-negative Staphylococcus spp.) Among positive specimens, the median P. acnes bacterial burden was 350 CFU/g (12 - similar to 20,000 CFU/g). Thirtyeight P. acnes isolates were subjected to molecular sub-typing, identifying 4 of 6 defined phylogroups: IA1, IB, IC, and II. Eight culture-positive specimens were evaluated by fluorescence microscopy and revealed P. acnes in situ. Notably, these bacteria demonstrated a biofilm distribution within the disc matrix. P. acnes bacteria were more prevalent in males than females (39% vs. 23%, p = 0.0013). Conclusions This study confirms that P. acnes is prevalent in herniated disc tissue. Moreover, it provides the first visual evidence of P. acnes biofilms within such specimens, consistent with infection rather than microbiologic contamination.

Návaznosti

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

MANU NATH CAPOOR, externista; Filip RŮŽIČKA; Jonathan E. SCHMITZ; Garth A. JAMES; Táňa MACHÁČKOVÁ; Radim JANČÁLEK; Martin SMRČKA; Radim LIPINA; externista FAHAD AHMED; Todd F. ALAMIN; Neel ANAND; John C. BAIRD; Nitin BHATIA; Sibel DEMIR-DEVIREN; Robert K. EASTLACK; Steve FISHER; Steven R. GARFIN; Jaspaul S. GOGIA; Ziya L. GOKASLAN; Calvin C. KUO; Yu-Po LEE; Konstantinos MAVROMMATIS; Elleni PONECHAL MICHU; Hana NOSKOVÁ; Assaf RAZ; Jiří ŠÁNA; A. Nick SHAMIE; Philip S. STEWART; Jerry L. STONEMETZ; Jeffrey C. WANG; Timothy F. WITHAM; Michael F. COSCIA; Christof BIRKENMAIER; Vincent A. FISCHETTI a Ondřej SLABÝ. Propionibacterium acnes biofilm is present in intervertebral discs of patients undergoing microdiscectomy. Plos one. San Francisco: Public Library of Science, 2017, roč. 12, č. 4, s. nestránkováno, 17 s. ISSN 1932-6203. Dostupné z: https://doi.org/10.1371/journal.pone.0174518.

@article{1382015,
   author = {Manu Nath Capoor, externista and Růžička, Filip and Schmitz, Jonathan E. and James, Garth A. and Macháčková, Táňa and Jančálek, Radim and Smrčka, Martin and Lipina, Radim and Fahad Ahmed, externista and Alamin, Todd F. and Anand, Neel and Baird, John C. and Bhatia, Nitin and DemirandDeviren, Sibel and Eastlack, Robert K. and Fisher, Steve and Garfin, Steven R. and Gogia, Jaspaul S. and Gokaslan, Ziya L. and Kuo, Calvin C. and Lee, YuandPo and Mavrommatis, Konstantinos and Ponechal Michu, Elleni and Nosková, Hana and Raz, Assaf and Šána, Jiří and Shamie, A. Nick and Stewart, Philip S. and Stonemetz, Jerry L. and Wang, Jeffrey C. and Witham, Timothy F. and Coscia, Michael F. and Birkenmaier, Christof and Fischetti, Vincent A. and Slabý, Ondřej},
   article_location = {San Francisco},
   article_number = {4},
   doi = {https://doi.org/10.1371/journal.pone.0174518},
   keywords = {LOW-BACK-PAIN; SAPHO SYNDROME; MODIC CHANGES; LUMBAR SPINE; SARCOIDOSIS; INFECTIONS; ASSOCIATION; CONTAMINATION; INVOLVEMENT; DIAGNOSIS},
   language = {eng},
   issn = {1932-6203},
   journal = {Plos one},
   title = {Propionibacterium acnes biofilm is present in intervertebral discs of patients undergoing microdiscectomy},
   url = {http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0174518},
   volume = {12},
   year = {2017}
}

TY  - JOUR
ID  - 1382015
AU  - Manu Nath Capoor, externista - Růžička, Filip - Schmitz, Jonathan E. - James, Garth A. - Macháčková, Táňa - Jančálek, Radim - Smrčka, Martin - Lipina, Radim - Fahad Ahmed, externista - Alamin, Todd F. - Anand, Neel - Baird, John C. - Bhatia, Nitin - Demir-Deviren, Sibel - Eastlack, Robert K. - Fisher, Steve - Garfin, Steven R. - Gogia, Jaspaul S. - Gokaslan, Ziya L. - Kuo, Calvin C. - Lee, Yu-Po - Mavrommatis, Konstantinos - Ponechal Michu, Elleni - Nosková, Hana - Raz, Assaf - Šána, Jiří - Shamie, A. Nick - Stewart, Philip S. - Stonemetz, Jerry L. - Wang, Jeffrey C. - Witham, Timothy F. - Coscia, Michael F. - Birkenmaier, Christof - Fischetti, Vincent A. - Slabý, Ondřej
PY  - 2017
TI  - Propionibacterium acnes biofilm is present in intervertebral discs of patients undergoing microdiscectomy
JF  - Plos one
VL  - 12
IS  - 4
SP  - nestránkováno
EP  - nestránkováno
PB  - Public Library of Science
SN  - 19326203
KW  - LOW-BACK-PAIN
KW  - SAPHO SYNDROME
KW  - MODIC CHANGES
KW  - LUMBAR SPINE
KW  - SARCOIDOSIS
KW  - INFECTIONS
KW  - ASSOCIATION
KW  - CONTAMINATION
KW  - INVOLVEMENT
KW  - DIAGNOSIS
UR  - http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0174518
L2  - http://journals.plos.org/plosone/article?id=10.1371/journal.pone.0174518
N2  - Background In previous studies, Propionibacterium acnes was cultured from intervertebral disc tissue of similar to 25% of patients undergoing microdiscectomy, suggesting a possible link between chronic bacterial infection and disc degeneration. However, given the prominence of P. acnes as a skin commensal, such analyses often struggled to exclude the alternate possibility that these organisms represent perioperative microbiologic contamination. This investigation seeks to validate P. acnes prevalence in resected disc cultures, while providing microscopic evidence of P. acnes biofilm in the intervertebral discs. Methods Specimens from 368 patients undergoing microdiscectomy for disc herniation were divided into several fragments, one being homogenized, subjected to quantitative anaerobic culture, and assessed for bacterial growth, and a second fragment frozen for additional analyses. Colonies were identified by MALDI-TOF mass spectrometry and P. acnes phylotyping was conducted by multiplex PCR. For a sub-set of specimens, bacteria localization within the disc was assessed by microscopy using confocal laser scanning and FISH. Results Bacteria were cultured from 162 discs (44%), including 119 cases (32.3%) with P. acnes. In 89 cases, P. acnes was cultured exclusively; in 30 cases, it was isolated in combination with other bacteria (primarily coagulase-negative Staphylococcus spp.) Among positive specimens, the median P. acnes bacterial burden was 350 CFU/g (12 - similar to 20,000 CFU/g). Thirtyeight P. acnes isolates were subjected to molecular sub-typing, identifying 4 of 6 defined phylogroups: IA1, IB, IC, and II. Eight culture-positive specimens were evaluated by fluorescence microscopy and revealed P. acnes in situ. Notably, these bacteria demonstrated a biofilm distribution within the disc matrix. P. acnes bacteria were more prevalent in males than females (39% vs. 23%, p = 0.0013). Conclusions This study confirms that P. acnes is prevalent in herniated disc tissue. Moreover, it provides the first visual evidence of P. acnes biofilms within such specimens, consistent with infection rather than microbiologic contamination.
ER  -

MANU NATH CAPOOR, externista; Filip RŮŽIČKA; Jonathan E. SCHMITZ; Garth A. JAMES; Táňa MACHÁČKOVÁ; Radim JANČÁLEK; Martin SMRČKA; Radim LIPINA; externista FAHAD AHMED; Todd F. ALAMIN; Neel ANAND; John C. BAIRD; Nitin BHATIA; Sibel DEMIR-DEVIREN; Robert K. EASTLACK; Steve FISHER; Steven R. GARFIN; Jaspaul S. GOGIA; Ziya L. GOKASLAN; Calvin C. KUO; Yu-Po LEE; Konstantinos MAVROMMATIS; Elleni PONECHAL MICHU; Hana NOSKOVÁ; Assaf RAZ; Jiří ŠÁNA; A. Nick SHAMIE; Philip S. STEWART; Jerry L. STONEMETZ; Jeffrey C. WANG; Timothy F. WITHAM; Michael F. COSCIA; Christof BIRKENMAIER; Vincent A. FISCHETTI a Ondřej SLABÝ. Propionibacterium acnes biofilm is present in intervertebral discs of patients undergoing microdiscectomy. \textit{Plos one}. San Francisco: Public Library of Science, 2017, roč.~12, č.~4, s.~nestránkováno, 17 s. ISSN~1932-6203. Dostupné z: https://doi.org/10.1371/journal.pone.0174518.

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