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@inproceedings{1382509,
author = {Třísková, Iveta and Bosáková, Markéta and Trnková, Libuše},
address = {Brno},
booktitle = {XVII. Workshop of Physical Chemists and Electrochemists},
edition = {1},
editor = {Libuše Trnková},
keywords = {epigenetic modifications; DNA methylation; methylguanines; voltammetry; PeGE},
howpublished = {elektronická verze "online"},
language = {eng},
location = {Brno},
isbn = {978-80-210-8580-0},
pages = {50-51},
publisher = {Masarykova univerzita},
title = {PENCIL GRAPHITE ELECTRODE AS A PROMISING TOOL FOR ELECTROANALYSIS OF METHYLGUANINES},
year = {2017}
}
TY - JOUR
ID - 1382509
AU - Třísková, Iveta - Bosáková, Markéta - Trnková, Libuše
PY - 2017
TI - PENCIL GRAPHITE ELECTRODE AS A PROMISING TOOL FOR ELECTROANALYSIS OF METHYLGUANINES
PB - Masarykova univerzita
CY - Brno
SN - 9788021085800
KW - epigenetic modifications
KW - DNA methylation
KW - methylguanines
KW - voltammetry
KW - PeGE
N2 - Nowadays, epigenetics is among the top disciplines of biomedicine. It is actually because epigentic modifications, especially methylation of nucleic acids (NAs), are linked with many diseases and dysfunctions, such as human carcinomas (lung, thyroid, prostate and pancreas), leukemia, etc.[1-4]. Guanine (G), one of the two essential nucleic bases, plays a key role in the DNA oxidation by various types of oxidants and free radicals [5-7]. Although guanine, compared with cytosine, is not a typical recipient of methyl groups, methylated guanines attract the attention as potential drugs and/or markers. Specifically, N7-methyl modification of G is a well-established biomarker for the detection and determination of DNA methylation [8]. Only few examples can be found in the literature regarding to the electrochemical determination of this alkylmodification of G. The electrochemical reduction of 7-methylguanine derivatives (7-methylguanosine and 7-methylguanosine-5‘-phosphate) at the mercury electrode [3, 9, 10] was completed with the study of oxidation behavior of 7-methylguanine at boron-doped diamond electrode and carbon electrodes (glassy carbon electrode and screen printed graphite electrodes)[8]. There are some papers regarding electrochemical behavior of 7N-methylguanine (7N-mG), linked with epigenetic modifications of DNA chain, but there are not any publications regarding other methylated guanine derivatives, such as 1N-, 3N- or 9N-methylguanines. In our research the oxidation processes of methylated guanine derivatives on a polymer pencil graphite electrode (pPeGE) in dependence on pH (phosphate – acetate buffer; pH 3.16 – 7.52) and the position of methyl group in the G molecule (pyrimidine ring vs. imidazole ring) were investigated.
ER -
TŘÍSKOVÁ, Iveta, Markéta BOSÁKOVÁ a Libuše TRNKOVÁ. PENCIL GRAPHITE ELECTRODE AS A PROMISING TOOL FOR ELECTROANALYSIS OF METHYLGUANINES. Online. In Libuše Trnková. \textit{XVII. Workshop of Physical Chemists and Electrochemists}. 1. vyd. Brno: Masarykova univerzita, 2017. s.~50-51. ISBN~978-80-210-8580-0. [citováno 2024-04-23]
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