Expression of genes associated with BMP signaling pathway in
porcine oocytes before and after IVM - a microarray approach

J 2017

Expression of genes associated with BMP signaling pathway in porcine oocytes before and after IVM - a microarray approach

BUDNA, Joanna; Marta RYBSKA; Sylwia CIESIÓŁKA; Artur BRYJA; Sylwia BORYS et. al.

Základní údaje

Originální název

Expression of genes associated with BMP signaling pathway in porcine oocytes before and after IVM - a microarray approach

Autoři

Vydání

Reproductive Biology and Endocrinology, London, BioMed Central, 2017, 1477-7827

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

30202 Endocrinology and metabolism

Stát vydavatele

Velká Británie a Severní Irsko

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 2.852

Kód RIV

RIV/00216224:14110/17:00097069

Organizační jednotka

Lékařská fakulta

DOI

https://doi.org/10.1186/s12958-017-0261-6

UT WoS

000402578900002

EID Scopus

2-s2.0-85019986979

Klíčová slova anglicky

Pig; Oocytes; Microarray; In vitro maturation

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 20. 3. 2018 16:11, Soňa Böhmová

Anotace

V originále

Background: The full maturational capability of mammalian oocytes is accompanied by nuclear and cytoplasmic modifications, which are associated with proliferation and differentiation of surrounding cumulus cells. These events are regulated on molecular level by the expression of target genes involved in signal transduction pathways crucial for folliculogenesis and oogenesis. Transforming growth factor beta signaling includes several molecules that are involved in the regulation of oogenesis and embryo growth, including bone morphogenetic protein (BMP). However, the BMP-related gene expression profile in oocytes at different maturational stages requires further investigation. Methods: Oocytes were isolated from pubertal crossbred Landrace gilts follicles, selected with a use of BCB staining test and analyzed before and after in vitro maturation. Gene expression profiles were examined using an Affymetrix microarray approach and validated by RT-qPCR. Database for Annotation, Visualization, and Integrated Discovery (DAVID) software was used for the extraction of the genes belonging to a BMP-signaling pathway ontology group. Results: The assay revealed 12,258 different transcripts in porcine oocytes, among which 379 genes were down-regulated and 40 were up-regulated. The DAVID database indicated a "BMP signaling pathway" ontology group, which was significantly regulated in both groups of oocytes. We discovered five up-regulated genes in oocytes before versus after in vitro maturation (IVM): chordin-like 1 (CHRDL1), follistatin (FST), transforming growth factor-beta receptor-type III (TGF beta R3), decapentaplegic homolog 4 (SMAD4), and inhibitor of DNA binding 1 (ID1). Conclusions: Increased expression of CHRDL1, FST, TGF beta R3, SMAD4, and ID1 transcripts before IVM suggested a subordinate role of the BMP signaling pathway in porcine oocyte maturational competence. Conversely, it is postulated that these genes are involved in early stages of folliculogenesis and oogenesis regulation in pigs, since in oocytes before IVM increased expression was observed.

Citovat

BUDNA, Joanna; Marta RYBSKA; Sylwia CIESIÓŁKA; Artur BRYJA; Sylwia BORYS; Wiesława KRANC; Katarzyna WOJTANOWICZ-MARKIEWICZ; Michal JEŠETA; Eva SUMELKA; Dorota BUKOWSKA; Paweł ANTOSIK; Klaus P. BRÜSSOW; Małgorzata BRUSKA; Michał NOWICKI; Maciej ZABEL a Bartosz KEMPISTY. Expression of genes associated with BMP signaling pathway in porcine oocytes before and after IVM - a microarray approach. Reproductive Biology and Endocrinology. London: BioMed Central, 2017, roč. 15, č. 1, s. 1-9. ISSN 1477-7827. Dostupné z: https://doi.org/10.1186/s12958-017-0261-6.

@article{1384225,
   author = {Budna, Joanna and Rybska, Marta and Ciesiółka, Sylwia and Bryja, Artur and Borys, Sylwia and Kranc, Wiesława and WojtanowiczandMarkiewicz, Katarzyna and Ješeta, Michal and Sumelka, Eva and Bukowska, Dorota and Antosik, Paweł and Brüssow, Klaus P. and Bruska, Małgorzata and Nowicki, Michał and Zabel, Maciej and Kempisty, Bartosz},
   article_location = {London},
   article_number = {1},
   doi = {https://doi.org/10.1186/s12958-017-0261-6},
   keywords = {Pig; Oocytes; Microarray; In vitro maturation},
   language = {eng},
   issn = {1477-7827},
   journal = {Reproductive Biology and Endocrinology},
   title = {Expression of genes associated with BMP signaling pathway in porcine oocytes before and after IVM - a microarray approach},
   volume = {15},
   year = {2017}
}

TY  - JOUR
ID  - 1384225
AU  - Budna, Joanna - Rybska, Marta - Ciesiółka, Sylwia - Bryja, Artur - Borys, Sylwia - Kranc, Wiesława - Wojtanowicz-Markiewicz, Katarzyna - Ješeta, Michal - Sumelka, Eva - Bukowska, Dorota - Antosik, Paweł - Brüssow, Klaus P. - Bruska, Małgorzata - Nowicki, Michał - Zabel, Maciej - Kempisty, Bartosz
PY  - 2017
TI  - Expression of genes associated with BMP signaling pathway in porcine oocytes before and after IVM - a microarray approach
JF  - Reproductive Biology and Endocrinology
VL  - 15
IS  - 1
SP  - 1-9
EP  - 1-9
PB  - BioMed Central
SN  - 14777827
KW  - Pig
KW  - Oocytes
KW  - Microarray
KW  - In vitro maturation
N2  - Background: The full maturational capability of mammalian oocytes is accompanied by nuclear and cytoplasmic modifications, which are associated with proliferation and differentiation of surrounding cumulus cells. These events are regulated on molecular level by the expression of target genes involved in signal transduction pathways crucial for folliculogenesis and oogenesis. Transforming growth factor beta signaling includes several molecules that are involved in the regulation of oogenesis and embryo growth, including bone morphogenetic protein (BMP). However, the BMP-related gene expression profile in oocytes at different maturational stages requires further investigation. Methods: Oocytes were isolated from pubertal crossbred Landrace gilts follicles, selected with a use of BCB staining test and analyzed before and after in vitro maturation. Gene expression profiles were examined using an Affymetrix microarray approach and validated by RT-qPCR. Database for Annotation, Visualization, and Integrated Discovery (DAVID) software was used for the extraction of the genes belonging to a BMP-signaling pathway ontology group. Results: The assay revealed 12,258 different transcripts in porcine oocytes, among which 379 genes were down-regulated and 40 were up-regulated. The DAVID database indicated a "BMP signaling pathway" ontology group, which was significantly regulated in both groups of oocytes. We discovered five up-regulated genes in oocytes before versus after in vitro maturation (IVM): chordin-like 1 (CHRDL1), follistatin (FST), transforming growth factor-beta receptor-type III (TGF beta R3), decapentaplegic homolog 4 (SMAD4), and inhibitor of DNA binding 1 (ID1). Conclusions: Increased expression of CHRDL1, FST, TGF beta R3, SMAD4, and ID1 transcripts before IVM suggested a subordinate role of the BMP signaling pathway in porcine oocyte maturational competence. Conversely, it is postulated that these genes are involved in early stages of folliculogenesis and oogenesis regulation in pigs, since in oocytes before IVM increased expression was observed.
ER  -

BUDNA, Joanna; Marta RYBSKA; Sylwia CIESIÓŁKA; Artur BRYJA; Sylwia BORYS; Wiesława KRANC; Katarzyna WOJTANOWICZ-MARKIEWICZ; Michal JEŠETA; Eva SUMELKA; Dorota BUKOWSKA; Paweł ANTOSIK; Klaus P. BRÜSSOW; Małgorzata BRUSKA; Michał NOWICKI; Maciej ZABEL a Bartosz KEMPISTY. Expression of genes associated with BMP signaling pathway in porcine oocytes before and after IVM - a microarray approach. \textit{Reproductive Biology and Endocrinology}. London: BioMed Central, 2017, roč.~15, č.~1, s.~1-9. ISSN~1477-7827. Dostupné z: https://doi.org/10.1186/s12958-017-0261-6.

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