Další formáty:
BibTeX
LaTeX
RIS
@proceedings{1384294,
author = {Skrutková Langmajerová, Monika and Mijouin, Lily and Pichon, Chantal and Glatz, Zdeněk and Morin, Philippe and Nehmé, Reine},
booktitle = {HPLC 2017},
keywords = {skin fibroblast elastase, capillary electrophoresis, in capillary reaction},
title = {Activity of skin fibroblast elastase was online monitored by capillary electrophoresis},
url = {http://www.hplc2017-prague.org/},
year = {2017}
}
TY - CONF
ID - 1384294
AU - Skrutková Langmajerová, Monika - Mijouin, Lily - Pichon, Chantal - Glatz, Zdeněk - Morin, Philippe - Nehmé, Reine
PY - 2017
TI - Activity of skin fibroblast elastase was online monitored by capillary electrophoresis
KW - skin fibroblast elastase, capillary electrophoresis, in capillary reaction
UR - http://www.hplc2017-prague.org/
N2 - Skin fibroblast elastase (SFE) is one of the important enzymes involved in skin aging. It participates on the degradation of the elastic fibers which form a network that contributes to the elasticity of skin and their decreasing results in wrinkle formation. We examined activity of commercially available purified enzyme and cells lysate prepared from human normal fibroblasts and keratinocytes by capillary electrophoresis using in-capillary nanoreactor based on mixing by transverse diffusion of laminar flow profiles. In this study the 5-carboxyfluorescein labeled peptides were used as substrates. Their SFE cleavage resulted in fluorescently labeled products, which enabled to employ very specific and also sensitive LIF detection. All incubations in this study were performed by online nanoreactor to minimize reactant consumption.
ER -
SKRUTKOVÁ LANGMAJEROVÁ, Monika, Lily MIJOUIN, Chantal PICHON, Zdeněk GLATZ, Philippe MORIN a Reine NEHMÉ. Activity of skin fibroblast elastase was online monitored by capillary electrophoresis. In \textit{HPLC 2017}. 2017.
|
