Stress Response Monitoring of Photoautotrophic Higher Plant
Suspension Cultures by Fluorescence Imaging for High-Throughput
Toxic Compound Screening

J 2017

Stress Response Monitoring of Photoautotrophic Higher Plant Suspension Cultures by Fluorescence Imaging for High-Throughput Toxic Compound Screening

SEGEČOVÁ, Anna; Jan ČERVENÝ a Thomas ROITSCH

Základní údaje

Originální název

Stress Response Monitoring of Photoautotrophic Higher Plant Suspension Cultures by Fluorescence Imaging for High-Throughput Toxic Compound Screening

Autoři

SEGEČOVÁ, Anna; Jan ČERVENÝ a Thomas ROITSCH

Vydání

JOURNAL OF ENVIRONMENTAL PROTECTION, Scientific Research Publishing, 2017, 2152-2219

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10611 Plant sciences, botany

Stát vydavatele

Spojené státy

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/17:00108737

Organizační jednotka

Přírodovědecká fakulta

DOI

https://doi.org/10.4236/jep.2017.86044

Klíčová slova anglicky

Toxins; Toxicants; Ecotoxicology; PAM Chlorophyll Fluorescence

Štítky

rivok

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 9. 4. 2020 14:47, Mgr. Marie Novosadová Šípková, DiS.

Anotace

V originále

Photoautotrophic suspension cultures have been established from various model and crop plants and proved to be valuable and robust experimental system to assess coordinated responses of primary and secondary metabolism to metabolic and stress related signals. The use of suspension cultures combines the ease of handling microalgae in microtiter plates with the advantage of testing physiological responses of higher plants, notably in combination with the assessment of the response of photosynthetic activity by PAM chlorophyll fluorescence imaging as well as monitoring changes in secondary metabolite production and ROS formation by steady state fluorescence of plant fluorophores or introduced fluorescent probes. Photoautotrophic cultures provide various advantages as fast, highly sensitive, robust and high-through-put experimental system for screening and characterization of the impact of toxic compounds on higher plants. This opinion article discusses and critically evaluates the potential of photoautotrophic cultures of higher plants in combination with fluorescence imaging assays in microtiter plates as a complement to existing guidelines for testing the toxicity of chemicals in plants.

Citovat

SEGEČOVÁ, Anna; Jan ČERVENÝ a Thomas ROITSCH. Stress Response Monitoring of Photoautotrophic Higher Plant Suspension Cultures by Fluorescence Imaging for High-Throughput Toxic Compound Screening. JOURNAL OF ENVIRONMENTAL PROTECTION. Scientific Research Publishing, 2017, roč. 8, č. 6, s. 678-692. ISSN 2152-2219. Dostupné z: https://doi.org/10.4236/jep.2017.86044.

@article{1384330,
   author = {Segečová, Anna and Červený, Jan and Roitsch, Thomas},
   article_number = {6},
   doi = {https://doi.org/10.4236/jep.2017.86044},
   keywords = {Toxins; Toxicants; Ecotoxicology; PAM Chlorophyll Fluorescence},
   language = {eng},
   issn = {2152-2219},
   journal = {JOURNAL OF ENVIRONMENTAL PROTECTION},
   title = {Stress Response Monitoring of Photoautotrophic Higher Plant Suspension Cultures by Fluorescence Imaging for High-Throughput Toxic Compound Screening},
   url = {http://file.scirp.org/pdf/JEP_2017062714112100.pdf},
   volume = {8},
   year = {2017}
}

TY  - JOUR
ID  - 1384330
AU  - Segečová, Anna - Červený, Jan - Roitsch, Thomas
PY  - 2017
TI  - Stress Response Monitoring of Photoautotrophic Higher Plant Suspension Cultures by Fluorescence Imaging for High-Throughput Toxic Compound Screening
JF  - JOURNAL OF ENVIRONMENTAL PROTECTION
VL  - 8
IS  - 6
SP  - 678-692
EP  - 678-692
PB  - Scientific Research Publishing
SN  - 21522219
KW  - Toxins
KW  - Toxicants
KW  - Ecotoxicology
KW  - PAM Chlorophyll Fluorescence
UR  - http://file.scirp.org/pdf/JEP_2017062714112100.pdf
L2  - http://file.scirp.org/pdf/JEP_2017062714112100.pdf
N2  - Photoautotrophic suspension cultures have been established from various model and crop plants and proved to be valuable and robust experimental system to assess coordinated responses of primary and secondary metabolism to metabolic and stress related signals. The use of suspension cultures combines the ease of handling microalgae in microtiter plates with the advantage of testing physiological responses of higher plants, notably in combination with the assessment of the response of photosynthetic activity by PAM chlorophyll fluorescence imaging as well as monitoring changes in secondary metabolite production and ROS formation by steady state fluorescence of plant fluorophores or introduced fluorescent probes. Photoautotrophic cultures provide various advantages as fast, highly sensitive, robust and high-through-put experimental system for screening and characterization of the impact of toxic compounds on higher plants. This opinion article discusses and critically evaluates the potential of photoautotrophic cultures of higher plants in combination with fluorescence imaging assays in microtiter plates as a complement to existing guidelines for testing the toxicity of chemicals in plants.
ER  -

SEGEČOVÁ, Anna; Jan ČERVENÝ a Thomas ROITSCH. Stress Response Monitoring of Photoautotrophic Higher Plant Suspension Cultures by Fluorescence Imaging for High-Throughput Toxic Compound Screening. \textit{JOURNAL OF ENVIRONMENTAL PROTECTION}. Scientific Research Publishing, 2017, roč.~8, č.~6, s.~678-692. ISSN~2152-2219. Dostupné z: https://doi.org/10.4236/jep.2017.86044.

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