Magnetic Separation Methods for the Detection of Mycobacterium
avium subsp paratuberculosis in Various Types of Matrices: A
Review

J 2017

Magnetic Separation Methods for the Detection of Mycobacterium avium subsp paratuberculosis in Various Types of Matrices: A Review

HUŠÁKOVÁ, Markéta; R DZIEDZINSKA a Iva SLANÁ

Základní údaje

Originální název

Magnetic Separation Methods for the Detection of Mycobacterium avium subsp paratuberculosis in Various Types of Matrices: A Review

Autoři

HUŠÁKOVÁ, Markéta; R DZIEDZINSKA a Iva SLANÁ

Vydání

Biomed Research International, New York, Hindawi Publishing Corporation, 2017, 2314-6133

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Impakt faktor

Impact factor: 2.583

Označené pro přenos do RIV

Změněno: 7. 8. 2017 12:23, Mgr. Markéta Hušáková, Ph.D.

Anotace

V originále

The main reasons to improve the detection of Mycobacterium avium subsp. paratuberculosis (MAP) are animal health and monitoring of MAP entering the food chain via meat, milk, and/or dairy products. Different approaches can be used for the detection of MAP, but the use of magnetic separation especially in conjunction with PCR as an end-point detection method has risen in past years. However, the extraction of DNA which is a crucial step prior to PCR detection can be complicated due to the presence of inhibitory substances. Magnetic separation methods involving either antibodies or peptides represent a powerful tool for selective separation of target bacteria from other nontarget microorganisms and inhibitory sample components. These methods enable the concentration of pathogens present in the initial matrix into smaller volume and facilitate the isolation of sufficient quantities of pure DNA. The purpose of this review was to summarize the methods based on the magnetic separation approach that are currently available for the detection of MAP in a broad range of matrices.

Citovat

HUŠÁKOVÁ, Markéta; R DZIEDZINSKA a Iva SLANÁ. Magnetic Separation Methods for the Detection of Mycobacterium avium subsp paratuberculosis in Various Types of Matrices: A Review. Biomed Research International. New York: Hindawi Publishing Corporation, 2017, 15 s. ISSN 2314-6133. Dostupné z: https://doi.org/10.1155/2017/5869854.

@article{1386606,
   author = {Hušáková, Markéta and Dziedzinska, R and Slaná, Iva},
   article_location = {New York},
   doi = {https://doi.org/10.1155/2017/5869854},
   language = {eng},
   issn = {2314-6133},
   journal = {Biomed Research International},
   title = {Magnetic Separation Methods for the Detection of Mycobacterium avium subsp paratuberculosis in Various Types of Matrices: A Review},
   url = {http://dx.doi.org/10.1155/2017/5869854},
   year = {2017}
}

TY  - JOUR
ID  - 1386606
AU  - Hušáková, Markéta - Dziedzinska, R - Slaná, Iva
PY  - 2017
TI  - Magnetic Separation Methods for the Detection of Mycobacterium avium subsp paratuberculosis in Various Types of Matrices: A Review
JF  - Biomed Research International
PB  - Hindawi Publishing Corporation
SN  - 23146133
UR  - http://dx.doi.org/10.1155/2017/5869854
L2  - http://dx.doi.org/10.1155/2017/5869854
N2  - The main reasons to improve the detection of Mycobacterium avium subsp. paratuberculosis (MAP) are animal health and monitoring of MAP entering the food chain via meat, milk, and/or dairy products. Different approaches can be used for the detection of MAP, but the use of magnetic separation especially in conjunction with PCR as an end-point detection method has risen in past years. However, the extraction of DNA which is a crucial step prior to PCR detection can be complicated due to the presence of inhibitory substances. Magnetic separation methods involving either antibodies or peptides represent a powerful tool for selective separation of target bacteria from other nontarget microorganisms and inhibitory sample components. These methods enable the concentration of pathogens present in the initial matrix into smaller volume and facilitate the isolation of sufficient quantities of pure DNA. The purpose of this review was to summarize the methods based on the magnetic separation approach that are currently available for the detection of MAP in a broad range of matrices.
ER  -

HUŠÁKOVÁ, Markéta; R DZIEDZINSKA a Iva SLANÁ. Magnetic Separation Methods for the Detection of Mycobacterium avium subsp paratuberculosis in Various Types of Matrices: A Review. \textit{Biomed Research International}. New York: Hindawi Publishing Corporation, 2017, 15 s. ISSN~2314-6133. Dostupné z: https://doi.org/10.1155/2017/5869854.

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