Vancomycin-resistant enterococci with vanA gene in treated
municipal wastewater and their association with human hospital
strains

J 2017

Vancomycin-resistant enterococci with vanA gene in treated municipal wastewater and their association with human hospital strains

ORAVCOVA, Veronika; Matúš MIHALČIN; Jana ZAKOVA; Lucie POSPISILOVA; Martina MASARIKOVA et al.

Základní údaje

Originální název

Vancomycin-resistant enterococci with vanA gene in treated municipal wastewater and their association with human hospital strains

Autoři

ORAVCOVA, Veronika; Matúš MIHALČIN ; Jana ZAKOVA; Lucie POSPISILOVA; Martina MASARIKOVA a Ivan LITERAK

Vydání

Science of the Total Environment, Amsterdam, Elsevier Science, 2017, 0048-9697

Další údaje

Jazyk

angličtina

Typ výsledku

Článek v odborném periodiku

Obor

10511 Environmental sciences

Stát vydavatele

Nizozemské království

Utajení

není předmětem státního či obchodního tajemství

Impakt faktor

Impact factor: 4.610

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14110/17:00097302

Organizační jednotka

Lékařská fakulta

Klíčová slova anglicky

Antibiotic resistance; Clostridium difficile; Glycopeptides; Gull; Hospital Wastewater treatment plant

Štítky

EL OK

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 17. 5. 2018 16:26, Soňa Böhmová

Anotace

V originále

Vancomycin-resistant enterococci (VRE) are pathogens of increasing medical importance. In Brno, Czech Republic, we collected 37 samples from the effluent of a wastewater treatment plant (WWTP), 21 surface swabs from hospital settings, and 59 fecal samples from hospitalized patients and staff. Moreover, we collected 284 gull cloacal swabs from the colony situated 35 km downstream the WWTP. Samples were cultured selectively. Enterococci were identified using MALDI-TOF MS, phenotypically tested for susceptibility to antibiotics, and by PCR for occurrence of resistance and virulence genes. Pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST) were used to examine genotypic diversity. VRE carrying the vanA gene were found in 32 (86%, n = 37) wastewater samples, from which we obtained 49 isolates: Enterococcus faecium (44) and Enterococcus gallinarum (2), Enterococcus casseliflavus (2), and Enterococcus raffinosus (1). From 33 (69%) of 48 inpatient stool samples, we obtained 39 vanA-carrying VRE, which belonged to E. faecium (33 isolates), Enterococcus faecalis (4), and Enterococcus raffinosus (2). Nearly one-third of the samples from hospital surfaces contained VRE with the vanA gene. VRE were not detected among gulls. Sixty-seven (84%, n = 80) E. faecium isolates carried virulence genes hyl and/or esp. Virulence of E. faecalis was encoded by gelE, asa1, and cylA genes. A majority of the E. faecium isolates belonged to the clinically important sequence types ST17 (WWTP: 10 isolates; hospital: 4 isolates), ST18 (9;8), and ST78 (5;0). The remaining isolates belonged to ST555 (2;0), ST262 (1;6), ST273 (3;0), ST275 (1;0), ST549 (2;0), ST19 (0;1), ST323 (3;0), and ST884 (7;17). Clinically important enterococci carrying the vanA gene were almost continually detectable in the effluent of the WWTP, indicating insufficient removal of VRE during wastewater treatment and permanent shedding of these antibiotic resistant pathogens into the environment from this source. This represents a risk of their transmission to the environment.

Návaznosti

ED1.1.00/02.0068, projekt VaV

Název: CEITEC - central european institute of technology

LQ1601, projekt VaV

Název: CEITEC 2020 (Akronym: CEITEC2020)

Investor: Ministerstvo školství, mládeže a tělovýchovy ČR, CEITEC 2020

Citovat

ORAVCOVA, Veronika; Matúš MIHALČIN; Jana ZAKOVA; Lucie POSPISILOVA; Martina MASARIKOVA a Ivan LITERAK. Vancomycin-resistant enterococci with vanA gene in treated municipal wastewater and their association with human hospital strains. Science of the Total Environment. Amsterdam: Elsevier Science, 2017, roč. 609, 31 December 2017, s. 633-643. ISSN 0048-9697. Dostupné z: https://doi.org/10.1016/j.scitotenv.2017.07.121.

@article{1386670,
   author = {Oravcova, Veronika and Mihalčin, Matúš and Zakova, Jana and Pospisilova, Lucie and Masarikova, Martina and Literak, Ivan},
   article_location = {Amsterdam},
   article_number = {31 December 2017},
   doi = {https://doi.org/10.1016/j.scitotenv.2017.07.121},
   keywords = {Antibiotic resistance; Clostridium difficile; Glycopeptides; Gull; Hospital Wastewater treatment plant},
   language = {eng},
   issn = {0048-9697},
   journal = {Science of the Total Environment},
   title = {Vancomycin-resistant enterococci with vanA gene in treated municipal wastewater and their association with human hospital strains},
   volume = {609},
   year = {2017}
}

TY  - JOUR
ID  - 1386670
AU  - Oravcova, Veronika - Mihalčin, Matúš - Zakova, Jana - Pospisilova, Lucie - Masarikova, Martina - Literak, Ivan
PY  - 2017
TI  - Vancomycin-resistant enterococci with vanA gene in treated municipal wastewater and their association with human hospital strains
JF  - Science of the Total Environment
VL  - 609
IS  - 31 December 2017
SP  - 633-643
EP  - 633-643
PB  - Elsevier Science
SN  - 00489697
KW  - Antibiotic resistance
KW  - Clostridium difficile
KW  - Glycopeptides
KW  - Gull
KW  - Hospital Wastewater treatment plant
N2  - Vancomycin-resistant enterococci (VRE) are pathogens of increasing medical importance. In Brno, Czech Republic, we collected 37 samples from the effluent of a wastewater treatment plant (WWTP), 21 surface swabs from hospital settings, and 59 fecal samples from hospitalized patients and staff. Moreover, we collected 284 gull cloacal swabs from the colony situated 35 km downstream the WWTP. Samples were cultured selectively. Enterococci were identified using MALDI-TOF MS, phenotypically tested for susceptibility to antibiotics, and by PCR for occurrence of resistance and virulence genes. Pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST) were used to examine genotypic diversity. VRE carrying the vanA gene were found in 32 (86%, n = 37) wastewater samples, from which we obtained 49 isolates: Enterococcus faecium (44) and Enterococcus gallinarum (2), Enterococcus casseliflavus (2), and Enterococcus raffinosus (1). From 33 (69%) of 48 inpatient stool samples, we obtained 39 vanA-carrying VRE, which belonged to E. faecium (33 isolates), Enterococcus faecalis (4), and Enterococcus raffinosus (2). Nearly one-third of the samples from hospital surfaces contained VRE with the vanA gene. VRE were not detected among gulls. Sixty-seven (84%, n = 80) E. faecium isolates carried virulence genes hyl and/or esp. Virulence of E. faecalis was encoded by gelE, asa1, and cylA genes. A majority of the E. faecium isolates belonged to the clinically important sequence types ST17 (WWTP: 10 isolates; hospital: 4 isolates), ST18 (9;8), and ST78 (5;0). The remaining isolates belonged to ST555 (2;0), ST262 (1;6), ST273 (3;0), ST275 (1;0), ST549 (2;0), ST19 (0;1), ST323 (3;0), and ST884 (7;17). Clinically important enterococci carrying the vanA gene were almost continually detectable in the effluent of the WWTP, indicating insufficient removal of VRE during wastewater treatment and permanent shedding of these antibiotic resistant pathogens into the environment from this source. This represents a risk of their transmission to the environment.
ER  -

ORAVCOVA, Veronika; Matúš MIHALČIN; Jana ZAKOVA; Lucie POSPISILOVA; Martina MASARIKOVA a Ivan LITERAK. Vancomycin-resistant enterococci with vanA gene in treated municipal wastewater and their association with human hospital strains. \textit{Science of the Total Environment}. Amsterdam: Elsevier Science, 2017, roč.~609, 31 December 2017, s.~633-643. ISSN~0048-9697. Dostupné z: https://doi.org/10.1016/j.scitotenv.2017.07.121.

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