xMAP TECHNOLOGY: DETECTION OF PARASITIC AGENTS

k 2017

xMAP TECHNOLOGY: DETECTION OF PARASITIC AGENTS

RESLOVÁ, Nikol; Lucie ŠKORPÍKOVÁ; Karolína SNÍŽKOVÁ; Martin KAŠNÝ; Petr KRÁLÍK et al.

Základní údaje

Originální název

xMAP TECHNOLOGY: DETECTION OF PARASITIC AGENTS

Autoři

RESLOVÁ, Nikol; Lucie ŠKORPÍKOVÁ; Karolína SNÍŽKOVÁ; Martin KAŠNÝ a Petr KRÁLÍK

Vydání

23rd Helminthological Days, 2017

Další údaje

Jazyk

angličtina

Typ výsledku

Prezentace na konferencích

Obor

10600 1.6 Biological sciences

Stát vydavatele

Slovensko

Utajení

není předmětem státního či obchodního tajemství

Odkazy

URL

Označené pro přenos do RIV

Ano

Kód RIV

RIV/00216224:14310/17:00097334

Organizační jednotka

Přírodovědecká fakulta

ISBN

978-80-968473-8-9

Klíčová slova anglicky

xMAP; magnetic microspheres; parasites; molecular detection; diagnosis

Příznaky

Mezinárodní význam, Recenzováno

Změněno: 15. 8. 2017 13:38, Mgr. Nikol Reslová, Ph.D.

Anotace

V originále

Parasitic zoonoses are recorded worldwide and some of them have endemic character. Parasitic agents may pass from animals to humans in several ways, e.g., by direct contact, vector, consumption of raw or undercooked foodstuffs containing the infective stages or by infective stages released into environment. Although in the last decades a number of novel diagnostic methodological approaches have been developed, the current diagnosis of some parasitic diseases is still based only on a combination of clinical signs, anamnesis, and direct visual identification of parasitological objects. The most common conventional diagnostic methods, such as microscopic examination, biochemical assays, ELISA or PCR, are available, but they are laborious, time-consuming and in many cases not reliable. With regard to the fact that parasites might exhibit very strictly confined localization within the host’s body – intracellular/extracellular or tissue/organ, sampling can be very problematic and it often leads to a false negative results. Therefore, there is niche for the development of more sensitive diagnostic assays. Microsphere-based assays utilizing xMAP technology are applicable for high-throughput, multiplex and simultaneous detection of different analytes within a single complex sample. xMAP multiplex assays are currently available in various nucleic acid and immunoassay formats, enabling simultaneous detection and typing of pathogenic agents and also antigen or antibody interception. As an open architecture platform, the xMAP technology is beneficial to end users and therefore it is used in various pharmaceutical, clinical and research laboratories. The aim of our research is to exploit a potential of this approach to develop a multiplex oligonucleotide ligation PCR assay (MOL-PCR) determined for detection of nucleic acids in diagnosis of intestinal parasites in areas where co-infections are common and to upgrade conventional singleplex ELISA into the multiplex level.

Návaznosti

MUNI/A/1362/2016, interní kód MU

Název: Biologická diverzita – analýzy biologických systémů různých úrovní a na různých škálách prostředí

Investor: Masarykova univerzita, Biologická diverzita – analýzy biologických systémů různých úrovní a na různých škálách prostředí, DO R. 2020_Kategorie A - Specifický výzkum - Studentské výzkumné projekty

Citovat

RESLOVÁ, Nikol; Lucie ŠKORPÍKOVÁ; Karolína SNÍŽKOVÁ; Martin KAŠNÝ a Petr KRÁLÍK. xMAP TECHNOLOGY: DETECTION OF PARASITIC AGENTS. In 23rd Helminthological Days. 2017. ISBN 978-80-968473-8-9.

@proceedings{1387327,
   author = {Reslová, Nikol and Škorpíková, Lucie and Snížková, Karolína and Kašný, Martin and Králík, Petr},
   booktitle = {23rd Helminthological Days},
   keywords = {xMAP; magnetic microspheres; parasites; molecular detection; diagnosis},
   language = {eng},
   isbn = {978-80-968473-8-9},
   title = {xMAP TECHNOLOGY: DETECTION OF PARASITIC AGENTS},
   url = {http://pau.saske.sk/helminthdays2017/},
   year = {2017}
}

TY  - CONF
ID  - 1387327
AU  - Reslová, Nikol - Škorpíková, Lucie - Snížková, Karolína - Kašný, Martin - Králík, Petr
PY  - 2017
TI  - xMAP TECHNOLOGY: DETECTION OF PARASITIC AGENTS
SN  - 9788096847389
KW  - xMAP
KW  - magnetic microspheres
KW  - parasites
KW  - molecular detection
KW  - diagnosis
UR  - http://pau.saske.sk/helminthdays2017/
N2  - Parasitic zoonoses are recorded worldwide and some of them have endemic character. Parasitic agents may pass from animals to humans in several ways, e.g., by direct contact, vector, consumption of raw or undercooked foodstuffs containing the infective stages or by infective stages released into environment. Although in the last decades a number of novel diagnostic methodological approaches have been developed, the current diagnosis of some parasitic diseases is still based only on a combination of clinical signs, anamnesis, and direct visual identification of parasitological objects. The most common conventional diagnostic methods, such as microscopic examination, biochemical assays, ELISA or PCR, are available, but they are laborious, time-consuming and in many cases not reliable. With regard to the fact that parasites might exhibit very strictly confined localization within the host’s body – intracellular/extracellular or tissue/organ, sampling can be very problematic and it often leads to a false negative results. Therefore, there is niche for the development of more sensitive diagnostic assays. Microsphere-based assays utilizing xMAP technology are applicable for high-throughput, multiplex and simultaneous detection of different analytes within a single complex sample. xMAP multiplex assays are currently available in various nucleic acid and immunoassay formats, enabling simultaneous detection and typing of pathogenic agents and also antigen or antibody interception. As an open architecture platform, the xMAP technology is beneficial to end users and therefore it is used in various pharmaceutical, clinical and research laboratories. The aim of our research is to exploit a potential of this approach to develop a multiplex oligonucleotide ligation PCR assay (MOL-PCR) determined for detection of nucleic acids in diagnosis of intestinal parasites in areas where co-infections are common and to upgrade conventional singleplex ELISA into the multiplex level.
ER  -

RESLOVÁ, Nikol; Lucie ŠKORPÍKOVÁ; Karolína SNÍŽKOVÁ; Martin KAŠNÝ a Petr KRÁLÍK. xMAP TECHNOLOGY: DETECTION OF PARASITIC AGENTS. In \textit{23rd Helminthological Days}. 2017. ISBN~978-80-968473-8-9.

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